Atomic force microscopy study on chlorpromazine‐induced morphological changes of living HeLa cells In Vitro

Abstract

Chlorpromazine (CPZ)-induced morphological changes of living human cervical carcinoma cells were investigated by atomic force microscopy in near physiological condition. The results showed that the cell morphology changed visibly with time in the presence of CPZ (>21 microM). The cell membrane shrank gradually and detached finally from the substrate. After being treated with CPZ for 50 min, the cell volume increased by about 27.6% while its projective area (cell adhered to the substrate) decreased by about 12%. The mechanism was also discussed.

Atomic force microscopy imaging of live mammalian cells

“…In fact, we can see that in Figure 1 cell locomotion was not obvious 30 min after the retraction. Generally, cells were still alive 3 h after the AFM experiments [24,25]. Here we performed acquired AFM images within 3 h to insure that the cells were still alive.…”

基于afm的哺乳动物活细胞成像

“…时延 AFM 直接对 活体状态的样本进行纳米精度的观测, 因此为研究 细胞迁移过程中的动态过程提供了有用的工具. [24,25] , 因此在 3 h 内进行 AFM 实验以确保细胞仍然是活体状态. 然而, 造成 MCF-7 目前利用 AFM 对活体状态的哺乳动物细胞成像的分 辨率限制在 50 nm [6] , 这是由于细胞膜的动态柔软特 性使得扫描针尖很容易导致细胞膜的形变 [29] , 影响 到成像分辨率.…”

Surface plasmon-based infrared spectroscopy for cell biosensing

“…33 To track dynamics of this process through SP resonance we exposed a confluent human melanoma (MEL 1106) monolayer to 10 μM of CPZ. Figure 8(a) (upper panel) shows that λ sp is blue-shifted after CPZ introduction.…”

“…This result is consistent with other studies. 32,33 Explicitly, the λ sp measures penetration of extracellular medium between the cells and the substrate. Figure 8(b) schematically shows the cell morphological change induced by CPZ.…”