Atomic force microscopy studies of lateral phase separation in mixed monolayers of dipalmitoylphosphatidylcholine and dilauroylphosphatidylcholine

Sanchez, Jacqueline; Badia, Antonella

doi:10.1016/s0040-6090(03)00584-4

Cited by 49 publications

(46 citation statements)

References 95 publications

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“…53 mN·m -1 , very close to the pure saturated MGDG, therefore this argument can be used to elucidate the expulsion of one of the components in a mixed film. Maintaining temperature and external pressure constant, the number of degrees of freedom F of the monolayer system is given by the equation (6) [28,49,50]:…”

Section: Discussionmentioning

confidence: 99%

Biomimetic monolayer films of monogalactosyldiacylglycerol incorporating ubiquinone

Hoyo

Torrent‐Burgués

Guaus

2012

Journal of Colloid and Interface Science

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Ubiquinone and plastoquinone are two of the main electron and proton shuttle molecules in biological systems, and monogalactosyldiacylglycerol (MGDG) is the most abundant lipid in the thylakoid membrane of chloroplasts. Saturated MGDG, ubiquinone-10 (UQ) and MGDG:UQ mixed monolayers at the air/water interface have been studied using Surface Pressure-Area isotherms and Brewster Angle Microscopy. Moreover, the transferred Langmuir-Blodgett films have been observed by Atomic Force Microscopy. The results show that MGDG:UQ mixtures present more fluid phase than pure MGDG indicating a higher order degree for the later. It is also observed an important influence of UQ on the MGDG matrix before UQ collapse pressure and a low influence after this event, due to UQ expulsion from the MGDG matrix. This expulsion leads to a similar remaining UQ content for all the tested mixtures, indicating a limiting content of this molecule in the MGDG matrix at high surface pressures. The thermodynamic studies confirm the stability of the MGDG:UQ mixtures at low surface pressures, although presenting a non-ideal behaviour. Results point to consider UQ as a good candidate for studies of artificial photosynthesis.

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Section: Discussionmentioning

confidence: 99%

Biomimetic monolayer films of monogalactosyldiacylglycerol incorporating ubiquinone

Hoyo

Torrent‐Burgués

Guaus

2012

Journal of Colloid and Interface Science

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“…For example, DG exc was reported to be approximately À600, À1200, À1900, and 1000 J mol À1 at X additive = 0.3 and p % 30 mN m À1 for DPPC/cholesterol, DPPC/dipalmitoylphosphatic acid (DPPA), DPPC/dihexadecylphosphate (DHPS), and DPPC/dilauroylphosphatidylcholine (DLPC) monolayers, respectively. [24,[35][36][37] The small values of DG exc for the DPPC/C 60 system connote that the attractive interaction between the two components is very weak, but the miscibility increases with increasing surface pressure, probably due to enhanced many-body interactions at high film densities. [11][12][13] Atomic force microscopy images have clearly shown the DPPC monolayers in different phases (LE and LC; see Figure S2 of the Supporting Information) which were transferred to freshly cleaved mica surfaces at defined surface pressures.…”

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confidence: 99%

Effects of Fullerenes on Phospholipid Membranes: A Langmuir Monolayer Study

Wang

Yang

2009

ChemPhysChem

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We investigate two-component Langmuir monolayers of dipalmitoylphosphatidylcholine (DPPC)/C(60) by recording surface pressure/area (pi/A) and surface potential/area (DeltaV/A) isotherms and by direct Brewster angle microscopy (BAM) imaging. Atomic force microscopy (AFM) is employed to study morphologies of the mixed monolayers transferred to a solid substrate by the Langmuir-Blodgett technique. C(60) is shown to have little influence on isotherms of the DPPC/C(60) monolayers even at a molar fraction as high as X(C60)=0.3. The elastic modulus (Cs(-1) versus pi curves of the DPPC/C(60) monolayers almost overlay each other, as well as that of pure DPPC, that is, the elasticities of pure DPPC monolayers and DPPC/C(60) monolayers are remarkably similar. AFM studies reveal that fullerene flocs form at low surface pressures (pi

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“…No horizontal plateau was observed in any of the isotherms but a kink, or a change in slope, could be detected for the (25:75) mixture. The collapse pressures which varied between 50 and 62 mN m −1 were relatively constant, possibly indicating a low miscibility (Sanchez and Badia, 2003).…”

Section: Isothermsmentioning

confidence: 97%

Temperature and pressure dependent growth and morphology of DMPC/DSPC domains studied by Brewster angle microscopy

Arnold

Cloutier

Ritcey

et al. 2005

Chemistry and Physics of Lipids

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Atomic force microscopy studies of lateral phase separation in mixed monolayers of dipalmitoylphosphatidylcholine and dilauroylphosphatidylcholine

Cited by 49 publications

References 95 publications

Biomimetic monolayer films of monogalactosyldiacylglycerol incorporating ubiquinone

Biomimetic monolayer films of monogalactosyldiacylglycerol incorporating ubiquinone

Effects of Fullerenes on Phospholipid Membranes: A Langmuir Monolayer Study

Temperature and pressure dependent growth and morphology of DMPC/DSPC domains studied by Brewster angle microscopy

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