Atomic force microscopy studies of the influence of convex and concave nanostructures on the adsorption of fibronectin

Elter, Patrick; Lange, Regina; Beck, Ulrich

doi:10.1016/j.colsurfb.2011.09.021

Cited by 30 publications

(21 citation statements)

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“…Giamblanco et al 8 fabricated chemically homogeneous nanostructured surfaces of variable local curvature, concluding that the local curvature was strongly correlated with the kinetic adsorption phase and rate of laminin adsorption on the nanostructured surfaces. The dependence of protein adsorption on nano-scale patterned surface was also confirmed in the AFM-based force spectroscopy experiments 9 and the radiolabeling experiments. 10 The fibronection tended to adsorb preferentially in the concave grooves on the surface of boron-doped silicon wafers.…”

Section: Introductionsupporting

confidence: 52%

“…10 The fibronection tended to adsorb preferentially in the concave grooves on the surface of boron-doped silicon wafers. 9 Similarly, the adsorbed fibrinogen was shown to be regularly distributed most on the flanks and valleys of the dot-like protrusions, but sprinkled throughout the flat surface of poly(dimethylsiloxane). 10 The current researches suggest that proteins are sensitive to the nanoscale environment of anchoring surface, probably even showing special affinity for certain type of nanostructures.…”

Section: Introductionmentioning

confidence: 94%

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Molecular modeling of fibronectin adsorption on topographically nanostructured rutile (110) surfaces

Guo

Chen

et al. 2016

Applied Surface Science

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To investigate the topographical dependency of protein adsorption, molecular dynamics simulations were employed to describe the adsorption behavior of the tenth type-III module of fibronectin (FN-III 10) on nanostructured rutile (110) surfaces. The results indicated that the residence time of adsorbed FN-III 10 largely relied on its binding mode (direct or indirect) with the substrate and the region for protein migration on the periphery (protrusion) or in the interior (cavity or groove) of nanostructures. In the direct binding mode, FN-III 10 molecules were found to be 'trapped' at the anchoring sites of rutile surface, or even penetrate deep into the interior of nanostructures, regardless of the presented geometrical features. In the indirect binding mode, FN-III 10 molecules were indirectly connected to the substrate via a hydrogen-bond network (linking FN-III 10 and interfacial hydrations). The facets created by nanostructures, which exerted restraints on protein migration, were suggested to play an important role in the stability of indirect FN-III 10-rutile binding. However, a doubly unfavorable situation-indirect FN-III 10-rutile connections bridged by a handful of mediating waters and few constraints on movement of protein provided by nanostructures-would result in an early desorption of protein.

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Section: Introductionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 94%

Molecular modeling of fibronectin adsorption on topographically nanostructured rutile (110) surfaces

Guo

Chen

et al. 2016

Applied Surface Science

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“…Serum provide cell adhesion proteins, such as fibronectin, which enhance cell spreading [32]. Elter et al [33] reported an increased adsorption of the cell adhesion protein fibronectin on nanostructured surfaces compared to smooth surfaces. The nanostructured topography of zein surfaces, observed by AFM (Figure 2a), may have enhanced adsorption of cell adhesion proteins.…”

Section: Effect Of Zein Coatings On Cell Spreading and Viabilitymentioning

confidence: 99%

Cell spreading and viability on zein films may be facilitated by transglutaminase

Cui

Liu

Padua

2016

Colloids and Surfaces B: Biointerfaces

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Zein is a biocompatible corn protein potentially useful in the development of biomaterials. In this study, the deposition of zein on oxygen plasma treated glass cover slips significantly enhanced cell spreading and viability. The mechanism for cellular response to zein coated surfaces was thought to involve the polyglutamine peptides on the zein structure. We hypothesized that zein was a substrate for tissue transglutaminase (tTG), an extracellular enzyme involved in cell-surface interactions. SDS-PAGE results suggested an interaction between zein and tTG, where zein was the glutamine donor. Cross-linking between zein and tTG may be the first step in successful cell adhesion and spreading.

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“…The locally varying electric potential induces varyingly strong ionic shielding, and differences are compensated in approximately 2 nm distance from the solid surface. Recently, a number of studies concerning the adsorption behavior of biomolecules on both stochastically and regularly topographically nanostructured biomaterial surfaces have reported a significantly increased amount of adsorbed proteins in comparison to planar surfaces [3][4][5][6]. Moreover, spatially resolved measurements by means of scanning forcemicroscopic methods facilitated the identification of preferential adsorption sites at concave regions of the nanotopographies [3,6].…”

Section: The Titanium-tio 2 -Electrolytementioning

confidence: 99%

“…In recent years, several studies have suggested that, besides various chemical functionalization methods, topographically nanostructured biomaterial surfaces are a promising approach for the regulation of protein adsorption. It was demonstrated that topographical nanostructures significantly affect the amount [3][4][5][6] and the biological function of adsorbed proteins [6][7][8][9]. Increased electric field strengths at sharp edges and spikes of the surface, geometric effects, and locally varying dispersion interactions are often discussed as possible explanations for this effect [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Simulation of the Electric Field Distribution Near a Topographically Nanostructured Titanium‐Electrolyte Interface: Influence of the Passivation Layer

Körtge

Elter

Lange

et al. 2013

Journal of Nanomaterials

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A major challenge in biomaterials research is the regulation of protein adsorption at metallic implant surfaces. Recently, a number of studies have shown that protein adsorption can be influenced by metallic nanotopographies, which are discussed to increase electric field strengths near sharp edges and spikes. Since many metallic biomaterials form a native passivation layer with semiconducting properties, we have analyzed the influence of this layer on the near-surface electric field distribution of a nanostructure using finite element simulations. The Poisson-Boltzmann equation was solved for a titanium nanostructure covered by a TiO2passivation layer in contact with a physiological NaCl solution (bulk concentration 0.137 mol/L). In contrast to a purely metallic nanostructure, the electric field strengths near sharp edges and spikes can be lower than in planar regions if a passivation layer is considered. Our results demonstrate that the passivation layer has a significant influence on the near-surface electric field distribution and must be considered for theoretical treatments of protein adsorption on passivated metals like titanium.

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Atomic force microscopy studies of the influence of convex and concave nanostructures on the adsorption of fibronectin

Cited by 30 publications

References 50 publications

Molecular modeling of fibronectin adsorption on topographically nanostructured rutile (110) surfaces

Molecular modeling of fibronectin adsorption on topographically nanostructured rutile (110) surfaces

Cell spreading and viability on zein films may be facilitated by transglutaminase

Simulation of the Electric Field Distribution Near a Topographically Nanostructured Titanium‐Electrolyte Interface: Influence of the Passivation Layer

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