Atomic force microscopy reveals the mechanical properties of breast cancer bone metastases

Chen, Xinyue; Hughes, Russell; Mullin, Nic; Hawkins, Rhoda J.; Holen, Ingunn; Brown, Nicola J.; Hobbs, Jamie K.

doi:10.1039/d1nr03900h

Cited by 8 publications

(10 citation statements)

References 43 publications

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“…The stiffness of the cellular surface influenced cell adhesion, as stiffer cells were hard to deform and retained more contact area as well as invasion. − We also measured the adhesion force for adherent colorectal cancer cells treated with either CLE or LE drugs. During a 4 h measurement, the adhesion force was 0.161 nN for LE-drug-treated cells compared with a 0.108 nN for CLE-treated cells.…”

Section: Resultsmentioning

confidence: 99%

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Zhou

Meng

et al. 2022

Langmuir

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Combination chemotherapy is recognized as a vital medical treatment for cancer, but it has not achieved clinical ideal effects of combination therapy. Herein, we designed a supramolecular combination chemotherapy strategy based on cucurbit[8]uril (CB[8]), which can be facilely assembled into dual platinum drugs. Interestingly, employing the CB[8] carrier led to a greater than 10-fold intracellular Pt content compared to that of dual drugs at 4 h, and the CB[8] complex (CLE) can enhance the infiltration of platinum drugs in colorectal tumor cells tremendously. The platinum drugs can be released from CLE through consuming more tumor biomarker spermidine. Through analyzing the nanomechanical property of the colorectal tumor cellular surface by bioscope AFM, it was revealed that CLE modified the property by decreasing the adhesion and increasing the stiffness. This study provided a facile and sensitive strategy for improving combination chemotherapy by supramolecular materials.

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Section: Resultsmentioning

confidence: 99%

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Zhou

Meng

et al. 2022

Langmuir

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“…Laboratory-based measurements of local tumor stiffness commonly involve ex vivo indentation techniques such as already mentioned atomic force microscopy or microindentation. 25 , 26 …”

Section: Methods Of Measuring Tumor Mechanical Propertiesmentioning

confidence: 99%

Biomechanical Aspects in Bone Tumor Engineering

Menshikh,

Banicevic,

Obradovic

et al. 2024

Tissue Engineering Part B: Reviews

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In the past decades, anticancer drug development brought the field of tumor engineering to a new level by the need of robust test systems. Simulating tumor microenvironment in vitro remains a challenge, and osteosarcoma—the most common primary bone cancer—is no exception. The growing evidence points to the inevitable connection between biomechanical stimuli and tumor chemosensitivity and aggressiveness, thus making this component of the microenvironment a mandatory requirement to the developed models. In this review, we addressed the question: is the “ in vivo – in vitro ” gap in osteosarcoma engineering bridged from the perspective of biomechanical stimuli? The most notable biomechanical cues in the tumor cell microenvironment are observed and compared in the contexts of in vivo conditions and engineered three-dimensional in vitro models. Impact statement The importance of biomechanical stimuli in three-dimensional in vitro models for drug testing is becoming more pronounced nowadays. This review might assist in understanding the key players of the biophysical environment of primary bone cancer and the current state of bone tumor engineering from this perspective.

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“…Indentation depth should not exceed 10% of the sample thickness. Arduino et al, 2022;Chen et al, 2021;Holuigue et al, 2022;Jamal et al, 2022;Liu et al, 2019;Tang et al, 2019 Parabolic Deptula et al, 2021;Han et al, 2022;Nardini et al, 2022;Nguyen & Liu, 2022;Offroy et al, 2020;Sun et al, 2021;Zhu et al, 2020;Zhu et al, 2021;Zhu et al, 2022;Zhu, Tian, et al, 2023 Pyramidal Any axisymmetric shape…”

Section: Model Indenter Shapementioning

confidence: 99%

“…Creep model, cannot describe the relaxation behavior well. Banks et al, 2011;Chen et al, 2021 Boltzmann (Zener)…”

Section: Banks Et Al 2011mentioning

confidence: 99%

“…There is also lung cancer, glioma, colon cancer, breast cancer, melanoma, etc. (Chen et al, 2021; Deptula et al, 2020; Panzetta et al, 2017; Park et al, 2023; Tsitlakidis et al, 2020). Based on these mechanical differences, many studies have been conducted to determine whether cells or tissues are cancerous by mechanical parameters such as stiffness, adhesion, Young's modulus, etc.…”

Section: Cancermentioning

confidence: 99%

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Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Liu,

Han,

Kong

et al. 2023

Microscopy Res & Technique

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Despite significant progress in human medicine, certain diseases remain challenging to promptly diagnose and treat. Hence, the imperative lies in the development of more exhaustive criteria and tools. Tissue and cellular mechanics exhibit distinctive traits in both normal and pathological states, suggesting that “force” represents a promising and distinctive target for disease diagnosis and treatment. Atomic force microscopy (AFM) holds great promise as a prospective clinical medical device due to its capability to concurrently assess surface morphology and mechanical characteristics of biological specimens within a physiological setting. This review presents a comprehensive examination of the operational principles of AFM and diverse mechanical models, focusing on its applications in investigating tissue and cellular mechanics associated with prevalent diseases. The findings from these studies lay a solid groundwork for potential clinical implementations of AFM.Research HighlightsBy examining the surface morphology and assessing tissue and cellular mechanics of biological specimens in a physiological setting, AFM shows promise as a clinical device to diagnose and treat challenging diseases.

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Atomic force microscopy reveals the mechanical properties of breast cancer bone metastases

Abstract: Mechanically dependent processes are essential in cancer metastases. However, reliable mechanical characterization of metastatic cancer remains challenging whilst maintaining the tissue complexity and an intact sample. Using atomic force microscopy,...

Cited by 8 publications

References 43 publications

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Biomechanical Aspects in Bone Tumor Engineering

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

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