Atomic force microscopy probing of cell elasticity

Кузнецова, Т. Г.; Стародубцева, М. Н.; Yegorenkov, N.I.; Чижик, С. А.; Жданов, Р. И.

doi:10.1016/j.micron.2007.06.011

Cited by 676 publications

(533 citation statements)

References 59 publications

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“…To date, a challenge to this type of research has been posed by the occasional difficulty of exactly discerning one specific type of cell from others when only cellular Young's modulus is used (Li et al, 2015). When researchers have used AFM to quantify the Young's modulus of diverse types of cells, the overlap in Young's modulus among them (Kuznetsova et al, 2007;Yu et al, 2011;Li et al, 2014a;Hayashi and Iwata, 2015) has proved to be an important issue. Our results, as seen in Figure 5, show that cellular relaxation time can be a useful complement to discern different types of cells when they display considerable overlap in cellular Young's modulus.…”

Section: Rusults and Discussionmentioning

confidence: 99%

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young's modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young's modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.

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Section: Rusults and Discussionmentioning

confidence: 99%

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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“…Cette classification concorde avec des résultats de recherche récents [23]. La technique de spectroscopie de force est à la base de la méthodologie utilisée par les biomécanicien de la cellule pour estimer le module de Young des cellules [24], cette partie de TP constitue donc une initiation à cette approche expérimentale plus complexe (une sonde colloïdale et un logiciel de traitement des données spécifique sont alors requis).…”

Section: B)unclassified

Formation en Nanosciences et Nanotechnologies : Un pas vers une «vraie » interdisciplinarité

Sellier

Planus

Dubois

et al. 2014

J3eA

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Résumé : En 2011, l'Université Joseph Fourier (UJF), s'appuyant sur la force de la recherche interdisciplinaire des laboratoires grenoblois dans les Nanosciences et Nanotechnologies, a ouvert deux majeures : un M1 « Physique fondamentale et Nanosciences » et un M1 « Chimie et Nanosciences ». Dans ce cadre, des Travaux Pratiques interdisciplinaires et interplateformes ont été mis en place afin d'illustrer de manière concrète la convergence de la physique, de la chimie et de la biologie à l'échelle micro/nanométrique qui s'appuie sur les micro et nanotechnologies. Cet article décrit en détail ces TP et expose leur intérêt pédagogique pour la formation des étudiants ainsi que pour des enseignants-chercheurs.Mots clés : Nanosciences, Nano(bio)technologies, Nanoparticules, Microscopie à Force Atomique, Microscopie Electronique, Microscopie à épi-fluorescence, Culture cellulaire, Cytosquelette, Micropattern, Nanomanipulation, retour d'expérience IntroductionDepuis plusieurs années l'Université Joseph Fourier (UJF) propose une mention « Nanosciences et nanotechnologies » de Master en 2 ème année [1] en filières Recherche (Nanophysique, Nanochimie et Nanobiotechnologie) ou Professionnelle (Nanoingéniérie). En s'appuyant sur la force de la recherche interdisciplinaire des laboratoires grenoblois dans les Nanosciences et Nanotechnologies, l'UJF a depuis 2011, date de début du nouveau contrat quinquennal, renforcé sa formation dans ce domaine. Ont donc été ouvertes deux majeures «Nano» : un M1 «Physique fondamentale et Nanosciences» destiné aux étudiants physiciens et un M1 «Chimie et Nanosciences» destiné aux étudiants physico-chimistes, chimistes et biochimistes. L'ensemble des effectifs des deux parcours s'élève à 24 étudiants à ce jour. Dans le cadre de ces deux nouveaux Master 1, des Travaux Pratiques interdisciplinaires et inter-plateformes ont été mis en place afin d'illustrer de manière concrète la convergence de la physique, de la chimie et de la biologie à l'échelle micro/nanométrique qui s'appuie sur les micro et nanotechnologies. Ces séries de TP (une vingtaine d'heures au total) s'intègrent dans une UE spécifique de 50h ou 72h (6ECTS ou 9ECTS) intitulée « Nanosciences » dont la partie cours est déclinée en deux versions, l'une pour les physiciens, l'autre pour les (bio)chimistes. Elles s'articulent autour des thématiques de Nanochimie, Nano-caractérisation et Nanobiophysique ; Ces séries de TP ainsi que les cours associés, sont assurés par des enseignants-chercheurs de chimie, biologie et physique spécialistes en nanosciences et nanotechnologies. Une première session de 8h ou 12h (deux ou trois séances de 4h de TP) porte sur la «synthèse de nanoparticules métalliques et leur nano-caractérisation par MEB et AFM». La partie Article publié par EDP Sciences et disponible sur le site http://www.j3ea.org ou http://dx

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“…Several new concepts, describing mechanical responses in cells, have emerged since the development of AFM-thanks to its ability to apply or record minute forces on individual cells. The force-distance curves obtained by the direct indentation of the cell surface with an AFM tip have been used to quantify the mechanical properties of cells in contexts relevant to physiological processes such as cell migration, contraction and division [37,39,40,45]. The overall mechanical characteristics of a cell are largely determined by its plasma membrane, as well as by the different molecular components of the internal cytoskeleton and external glycocalix.…”

Section: Mechanical Sensing Of Cellular Processesmentioning

confidence: 99%

“…Indeed, to preserve its integrity and adapt to its external environment, a cell modulates its cytoskeleton architecture, which is composed of actin filaments, microtubules and intermediate filaments. In addition, the mechanical characteristics of a cell are also sensitive to the lipid and protein content of the membrane, as well as their interactions with diverse components of the extracellular environment [27,35,38,40,[47][48][49][50]. The possibility of characterising the mechanical properties of cells in real-time opens up new avenues for a more detailed understanding of physiological processes.…”

Section: Mechanical Sensing Of Cellular Processesmentioning

confidence: 99%

“…AFM force experiments on living cells have also opened up new vistas in the investigation of the mechanical properties of individual cells and have demonstrated the importance of these mechanical properties to various physiological and physiopathological processes [17,19,[35][36][37][38][39][40]. This review will discuss some recent contributions of the AFM to cell physiology with a focus on the probing and nanomanipulation of structures in living cell.…”

Section: Introductionmentioning

confidence: 99%

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AFM as a tool to probe and manipulate cellular processes

Lamontagne

Cuerrier

Grandbois

2007

Pflugers Arch - Eur J Physiol

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The exploration of molecular processes governing physiological functions has significantly benefited from the emergence of novel nanoscaled techniques. Atomic force microscopy in force measurement mode can be used to investigate a multitude of cellular events in individual living cells with great sensitivity. Precise regions of the plasma membrane can be examined in relation to particular signalling pathways activated by a mechanical stimulus. Similarly, subtle cellular movements induced by biochemical activation of specific membrane receptors can be detected in real time with excellent temporal and spatial resolution. The possibility to challenge locally and mechanically cell surface receptors also provides information on the control exerted by a cell over individual adhesion sites. Overall, this information is vital for an in-depth understanding of mechanisms related to cellular movement and morphological regulation. Lastly, atomic force microscope-based nanomanipulations on living cells have recently been proposed as a tool to influence and monitor cellular homeostasis by introducing specific molecular entities into or extracting them from the cytoplasm of individual cells. This review provides detailed examples on how such atomic force microscopy experiments can be conducted to investigate processes relevant to cell physiology.

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Atomic force microscopy probing of cell elasticity

Cited by 676 publications

References 59 publications

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Formation en Nanosciences et Nanotechnologies : Un pas vers une «vraie » interdisciplinarité

AFM as a tool to probe and manipulate cellular processes

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