Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking

Burgara-Estrella, Alexel; Acosta-Elías, Mónica; Álvarez‐Bajo, O.; Silva‐Campa, Erika; Angulo‐Molina, Aracely; Rodríguez-Hernández, Iracema del C.; Sarabia-Sainz, Héctor M.; Escalante-Lugo, Víctor M.; Pedroza‐Montero, M.

doi:10.1039/d0ra01384f

Cited by 5 publications

(4 citation statements)

References 97 publications

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“…The reduction in hemoglobin level can be due to a suppressive effect of nicotine on erythropoiesis or the result of hemolysis, which was caused by excessive destruction of erythrocytes. Cigarette smoke, cotinine and nicotine altered erythrocyte membrane stability through the oxidation of proteins membrane-SH groups which is greater than other tissues this is what leads to hemolysis (Burgara-Estrella et al, 2020). Nicotine had striking effects on the white blood cell count and platelets.…”

Section: Haematological Parametersmentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

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We investigate the effects of green tea extract (GTE) on the attenuation of nicotine hematotoxicity, oxidative stress, inflammation and spleen and bone marrow structural lesions. Rats were treated by injecting nicotine (1,5mg/kg b.w. for 7 weeks) intraperitoneally and thereby supplementing GTE 2% orally to them. Haematological profiles, inflammation markers, neutrophil/lymphocyte (NLR), platelet/lymphocyte (PLR) and mean platelet volume (MPV/Plat) ratios-and erythrocyte sedimentation rate (ESR) were evaluated. Splenic levels of malondialdehyde (MDA), nitric oxide (NO), advanced protein oxidation products (AOPP) and catalase activity were measured. Femur bone and spleen were subjected to histological study. Nicotine-induced haematological abnormalities, a rise in the NLR and MPV/Plat ratios and ESR values with a drop in the PLR values compared to other experimental groups and leads to a significant increase in MDA, NO and AOPP levels-with a decrease in catalase activity compared to control groups. The bone marrow and spleen of nicotine exposed rats showed severe degenerative changes. GTE supplementation attenuates hematotoxicity, induce a decrease in the inflammation markers values, improved the levels of MDA, NO, AOPP and catalase activity and attenuate the adverse histological effects. GTE rich on polyphenols and flavonoids revealed by the in vitro study protects against the hazardous effects of nicotine.

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Section: Haematological Parametersmentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

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“…It can follow the evolution of biological events in real time, but sometimes the fluorescence signal from water or related aqueous substances may hinder the Raman signal. This analytical tool has been widely used to study blood and its components, ranging from basic research on hemoglobin oxygenation to forensic investigations (Kang et al, 2008;Huang et al, 2011;Santacruz-Gomez et al, 2014;Buckley et al, 2016;Acosta-Elías et al, 2017;Atkins et al, 2017;Gautam et al, 2018;Burgara-Estrella et al, 2020). In particular, the oxygenated and deoxygenated Raman resonant hemoglobin modes have been used as indicators to describe RBC's integrity (Wood et al, 2001).…”

Section: Storage-induced Alterations In Rbc and Their Characterizationmentioning

confidence: 99%

“…The rapid assessment of membrane nanomodifications in RBCs may be accomplished using AFM (Girasole et al, 2001(Girasole et al, , 2007Asghari-Khiavi et al, 2010;Santacruz-Gomez et al, 2014;Marzec et al, 2015;Acosta-Elías et al, 2017;Kozlova et al, 2018;Spyratou et al, 2019;Burgara-Estrella et al, 2020). This technique allows the tracking in short times with minimal sample preparation, during the blood storage, the progressive impairment of the spectrin tetramers network's dynamical properties connected to actin complexes, and the bilayer's integral proteins (Moroz et al, 2010;Dinarelli et al, 2018;Kozlova et al, 2018Kozlova et al, , 2019.…”

Section: Storage-induced Alterations In Rbc and Their Characterizationmentioning

confidence: 99%

Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review

et al. 2021

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The storage lesions and the irradiation of blood cellular components for medical procedures in blood banks are events that may induce nanochanges in the membrane of red blood cells (RBCs). Alterations, such as the formation of pores and vesicles, reduce flexibility and compromise the overall erythrocyte integrity. This review discusses the alterations on erythrocytic lipid membrane bilayer through their characterization by confocal scanning microscopy, Raman, scanning electron microscopy, and atomic force microscopy techniques. The interrelated experimental results may address and shed light on the correlation of biomechanical and biochemical transformations induced in the membrane and cytoskeleton of stored and gamma-irradiated RBC. To highlight the main advantages of combining these experimental techniques simultaneously or sequentially, we discuss how those outcomes observed at micro- and nanoscale cell levels are useful as biomarkers of cell aging and storage damage.

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“…In blood cell field, there are three important components in blood: White Blood Cells(WBC), Red Blood Cells(RBC), and Platelets [1]. The proportion and number of these blood cells are seriously affect the doctor's judgment of the illness [2,3]. Finding an automated algorithm based on deep convolutional neural network to detect blood cells accurately and efficiently can improve the effectiveness of the medical system [4].…”

Section: Introductionmentioning

confidence: 99%

TE-YOLOF: Tiny and efficient YOLOF for blood cell detection

Xu¹,

Li²,

Yang³

et al. 2021

Preprint

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Blood cell detection in microscopic images is an essential branch of medical image processing research. Since disease detection based on manual checking of blood cells is time-consuming and full of errors, testing of blood cells using object detectors with Deep Convolutional Neural Network can be regarded as a feasible solution. In this work, an object detector based on YOLOF has been proposed to detect blood cell objects such as red blood cells, white blood cells and platelets. This object detector is called TE-YOLOF, Tiny and Efficient YOLOF, and it is a One-Stage detector using dilated encoder to extract information from single-level feature maps. For increasing efficiency and flexibility, the EfficientNet Convolutional Neural Network is utilized as the backbone for the proposed object detector. Furthermore, the Depthwise Separable Convolution is applied to enhance the performance and minimize the parameters of the network. In addition, the Mish activation function is employed to increase the precision. Extensive experiments on the BCCD dataset prove the effectiveness of the proposed model, which is more efficient than other existing studies for blood cell detection.

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Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking

Abstract: Tobacco smoke contains several compounds with oxidant and pro-oxidant properties with the capability of producing structural changes in biomolecules, as well as cell damage.

Cited by 5 publications

References 97 publications

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Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review

TE-YOLOF: Tiny and efficient YOLOF for blood cell detection

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