Atomic Force Microscopy and Its Spectroscopic Combinations for Studying Self‐Assembled Nanostructures

Optical, structural, and biological properties of silver nanoclusters formed within the loop of a C-12 hairpin sequence

“…Briey, 5 mL of the AgNC@DNA solution were deposited on amino-propyl-silatrane (APS) modied mica for a total of 2 min. 19,20,36 Excess sample was washed with DI water and gently dried under a ow of high purity argon gas and under vacuum overnight. AFM images in air were then recorded with a 1.5 Hz scanning rate using TESPA-300 probes from Bruker Nano, Inc.…”

“…AFM imaging could be challenging method for unexperienced researchers due to specic surface chemistry required for reliable deposition of AgNC@DNA. 36 Other alternative methods to assess heterogeneity of the samples might be considered, such as reversedphase high-performance liquid chromatography (HPLC), 37 gel electrophoresis, 38 or Transmission Electron Microscopy (TEM). 39 2.6 Energy-dispersive X-ray spectroscopy (EDS) analysis AgNC@hpC 12 solutions prepared as described above were diluted to 50 mM aer ltration.…”