Atomic force microscopy (AFM) application to diatom study: review and perspectives

Luís, Ana Teresa; Hlúbiková, Daša; Vaché, Véronique; Choquet, Patrick; Hoffmann, Lucien; Éctor, Luc

doi:10.1007/s10811-017-1177-4

Cited by 23 publications

(14 citation statements)

References 60 publications

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“…Together with previous studies, these results demonstrate that AFM, performed in physiological conditions, is required to assess true topographical characterization of diatoms with limited artefacts. 27,29,[31][32][33][34] N. palea cells adhere preferentially on hydrophobic surfaces. As diatoms are known to colonize surfaces of various physico-chemical properties, 16,18 we compared the adhesive properties of N. palea cells towards hydrophobic and hydrophilic surfaces.…”

Section: Resultsmentioning

confidence: 99%

Probing the Adhesion of the Common Freshwater Diatom Nitzschia palea at Nanoscale

Laviale

Beaussart

Allen

et al. 2019

ACS Appl. Mater. Interfaces

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Freshwater biofilms play an essential ecological role, but they also adversely affect human activities through undesirable biofouling of artificial submerged structures. They form complex aggregates of microorganisms that colonize any type of substratum. In phototrophic biofilms, diatoms dominate in biomass and produce copious amount of extracellular polymeric substances (EPSs), making them efficient early colonizers. Therefore, a better understanding of diatoms adhesive properties is essential to develop new anti-biofouling strategies. In this context, we used atomic force microscopy (AFM) to decipher the topography and adhesive mechanisms of the common freshwater diatom Nitzschia palea. Images taken in physiological conditions revealed typical ultrastructural features with a few nanometers resolution. Using single-cell force spectroscopy, we showed that N. palea strongly adheres to hydrophobic surfaces as compared to hydrophilic ones. Chemical force spectroscopy with hydrophobic tips further confirmed that the adhesion is governed by surface-associated hydrophobic EPS distributed in clusters at the frustule surface, and mostly composed of (glyco)-lipids as revealed by Raman spectroscopy. Collectively, our results demonstrate that AFM-based nanoscopy, combined with Raman spectroscopy, is a powerful tool to provide new insights into the adhesion mechanisms of diatoms.

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Section: Resultsmentioning

confidence: 99%

Probing the Adhesion of the Common Freshwater Diatom Nitzschia palea at Nanoscale

Laviale

Beaussart

Allen

et al. 2019

ACS Appl. Mater. Interfaces

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show abstract

“…The described method helps to study porosity of diatoms using SEM images where areolae and cribral pores are well visible. It is a good alternative to the studying of valve topography using AFM [Luis et al 2017], because presented method does not require the cultivation of diatoms and gives the comparable results. Moreover presented method allows fulfilling retrospective analysis of species porosity from published images.…”

Section: Discussionmentioning

confidence: 99%

Estimation the porosity of the valves of centric diatom Minidiscus vodyanitskiyi Lyakh & Bedoshvili using SEM images

2019

Preprint

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The diatoms interact with the outer environment through the siliceous walls of a frustule. Because of that the surface area of the frustule determines the ability of diatoms to absorb and excrete material resources. Such as unicellular organisms exchange matter only through the pores in their cell wall, to find relationships between characteristics of material fluxes and surface area of microorganism cover that is penetrable for substance, it is necessary to estimate the total surface area of pores or a porosityrelative area of pores perforated frustule. In the paper we describe a method of estimating the porosity of a diatom valve using SEM images. The method is tested on SEM images of the valves of centric diatom Minidiscus vodyanitskiyi recently described from the Sea of Azov. The results show that the valves porosity is less than 5 % of the total valves area. This value is consistent with the relative perforation of land plants leaves, which is less than 3%. We hypothesize that such value of diatom valves porosity is usual for many other diatom species. To verify this hypothesis additional researches are necessary.

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“…The described method helps to study the relative area of perforations of diatoms using SEM images where areola foramens and cribral pores are well visible. It is a good alternative to the studying of valve topography using AFM (Luis et al 2017), because presented method does not require the cultivation of diatoms and gives the comparable results. Moreover presented method allows fulfilling retrospective analysis of the relative area of perforations from published images.…”

Section: Discussionmentioning

confidence: 99%

New Method of Estimation of the Relative Area of Perforations on Valves of Centric Diatoms Using Sem Images on the Example of Minidisus Vodyanitskiyi Lyakh Et Bedoshvili

Lyakh¹,

Bedoshvili

Shikhat³

2019

Ecol. Mont

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The diatoms interact with the environment through the siliceous frustule. The total area of frustule perforations determines the ability of diatom to exchange nutrients, gases and other matters. The aim of the present study was to estimate the area of perforations on the valve surface of a centric diatom. In the paper we describe a method for the estimation of the area of perforations on a diatom valve using SEM images. The method is tested on valves of centric diatom Minidiscus vodyanitskiyi Lyakh et Bedoshvili. The results show that the total area of cribral pores is less than 5% of the total valve area. This value is consistent with the relative perforation of land plants leaves, which is less than 3%. We hypothesize that such small valve area occupied by perforations is usual for many other centric diatom species. To verify this hypothesis additional researches are necessary.

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Atomic force microscopy (AFM) application to diatom study: review and perspectives

Cited by 23 publications

References 60 publications

Probing the Adhesion of the Common Freshwater Diatom Nitzschia palea at Nanoscale

Probing the Adhesion of the Common Freshwater Diatom Nitzschia palea at Nanoscale

Estimation the porosity of the valves of centric diatom Minidiscus vodyanitskiyi Lyakh & Bedoshvili using SEM images

New Method of Estimation of the Relative Area of Perforations on Valves of Centric Diatoms Using Sem Images on the Example of Minidisus Vodyanitskiyi Lyakh Et Bedoshvili

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