Atomic absorption spectroscopy for the determination of elements in medical biological samples

Christian, Gary D.

doi:10.1007/bfb0051600

Cited by 6 publications

(2 citation statements)

References 156 publications

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“…The nitric acid-based microwave digestion was carried out to extract the metals from the serum. Flame atomic absorption spectrophotometer (AAS) (Tokiya and Toda, 1977) was used to determine the serum zinc (Zn) and magnesium (Mg) concentration. For the calibration, NIST SRM 3100 series singleelement standard solution was used at various concentrations to have a standard graph.…”

Section: Serum Metal Analysismentioning

confidence: 99%

RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease

et al. 2021

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Parkinson’s disease (PD) is a progressive neurodegenerative disease with no definite molecular markers for diagnosis. Metal exposure may alter cellular proteins that contribute to PD. Exploring the cross-talk between metal and its binding proteins in PD could reveal a new strategy for PD diagnosis. We performed a meta-analysis from different PD tissue microarray datasets to identify differentially expressed genes (DEGs) common to the blood and brain. Among common DEGs, we extracted 280 metalloprotein-encoding genes to construct protein networks describing the regulation of metalloproteins in the PD blood and brain. From the metalloprotein network, we identified three important functional hubs. Further analysis shows 60S ribosomal protein L6 (RPL6), a novel intermediary molecule connecting the three hubs of the metalloproteins network. Quantitative real-time PCR analysis showed that RPL6 was downregulated in PD peripheral blood mononuclear cell (PBMC) samples. Simultaneously, trace element analysis revealed altered serum zinc and magnesium concentrations in PD samples. The Pearson’s correlation analysis shows that serum zinc and magnesium regulate the RPL6 gene expression in PBMC. Thus, metal-regulating RPL6 acts as an intermediary molecule connecting the three hubs that are functionally associated with PD. Overall our study explores the understanding of metal-mediated pathogenesis in PD, which provides a serum metal environment regulating the cellular gene expression that may light toward metal and gene expression-based biomarkers for PD diagnosis.

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Section: Serum Metal Analysismentioning

confidence: 99%

RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease

et al. 2021

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“…nopal (Opuntia ficus indica), Rodriguez-Garcia et al, (2007) reported that the major mineral components in this cactus was as follows: Calcium, Magnesium, Sodium, Manganese, Iron, Zinc, Potassium, and minor mineral components were: Lithium, Vanadium, Cobalt, Arsenic, Selenium, Cadmium, Thallium . AAS has been used also to analyze muscle tissue, blood, urine, hair, bones, among others (Martinez-Flores et al, 2002;Christian, 1972). In most cases complex nature of biological materials requires dry ashing followed by wet digestion with oxidizing acids, i.e.…”

Section: Introductionmentioning

confidence: 99%

Mineral Content and Physicochemical Properties in Female Rats Bone During Growing Stage

Hernández-Urbiola¹,

Giraldo-Betancur²,

Jiménez-Mendoza³

et al. 2012

Atomic Absorption Spectroscopy

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Atomic absorption spectrometry of trace metals in clinical pathology

Sunderman¹

1973

Human Pathology

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Atomic absorption spectroscopy for the determination of elements in medical biological samples

Cited by 6 publications

References 156 publications

RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease

RPL6: A Key Molecule Regulating Zinc- and Magnesium-Bound Metalloproteins of Parkinson’s Disease

Mineral Content and Physicochemical Properties in Female Rats Bone During Growing Stage

Atomic absorption spectrometry of trace metals in clinical pathology

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