Atomic absorption spectrophotometry of chromium in serum and urine with a modified Perkin-Elmer 603 atomic absorption spectrophotometer.

Kayne, Frederick J.; Komar, G.; Laboda, Henry M.; Vanderlinde, Raymond E.

doi:10.1093/clinchem/24.12.2151

Cited by 63 publications

(12 citation statements)

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“…Earlier it was believed that at least 10% of all Cr3+ ingested was absorbed (373,375,380), which, therefore, indicated that much Cr3+ in food existed as firm complexes. This conclusion was based on comparison of the above stated normal intake of Cr3+ and the normal urinary excretion, which was reported to be 5 to 10 ,ug/day (381,382). Recent determinations have, however, shown that the latter value is probably about 10 times in excess of the true value (383-391).…”

Section: Metabolism and Effects Of Similar Ionsmentioning

confidence: 96%

“…The remainder is ultraffiltrable and very likely bound to various complexforming small molecules. Information reFarding the normal concentration in serum, as with Al +, is contradictory (366,395-397), but recent values of about 0.15 ,ug/ L are most plausible (381,395). In that case, the concentration corresponds to 2 to 3% of the supposed molar concentration of A13+.…”

Section: Metabolism and Effects Of Similar Ionsmentioning

confidence: 99%

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Metabolism and possible health effects of aluminum.

Ganrot¹

1986

Environ Health Perspect

203

150

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Literature regarding the biochemistry of aluminum and eight similar ions is reviewed. Close and hitherto unknown similarities were found. A hypothetical model is presented for the metabolism, based on documented direct observations of Al3+ and analogies from other ions. Main characteristics are low intestinal absorption, rapid urinary excretion, and slow tissue uptake, mostly in skeleton and reticuloendothelial cells. Intracellular Al3+ is probably first confined in the lysosomes but then slowly accumulates in the cell nucleus and chromatin. Large, long-lived cells, e.g., neurons, may be the most liable to this accumulation. In heterochromatin, Al3+ levels can be found comparable to those used in leather tannage. It is proposed that an accumulation may take place at a subcellular level without any significant increase in the corresponding tissue concentration. The possible effects of this accumulation are discussed. As Al3+ is neurotoxic, the brain metabolism is most interesting. The normal and the lethally toxic brain levels of Al3+ are well documented and differ only by a factor of 3-10. The normal brain uptake of Al3+ is estimated from data on intestinal uptake of Al3+ and brain uptake of radionuclides of similar ions administered intravenously. The uptake is very slow, 1 mg in 36 years, and is consistent with an assumption that Al3+ taken up by the brain cannot be eliminated and is therefore accumulated. The possibility that Al3+ may cause or contribute to some specific diseases, most of them related to aging, is discussed with the proposed metabolic picture in mind.

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Section: Metabolism and Effects Of Similar Ionsmentioning

confidence: 96%

Section: Metabolism and Effects Of Similar Ionsmentioning

confidence: 99%

Metabolism and possible health effects of aluminum.

Ganrot¹

1986

Environ Health Perspect

203

150

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“…Lead, Arsenic, Mercury, and Selenium were estimated using atomic absorption spectrometer (AAS) with samples digestion done according to the method described by Kayne et al [22]. More so, the concentration of testosterone and glutathione peroxidase enzyme in the seminal plasma was determined using ELISA technique as described in the work done by Adele et al [23].…”

Section: Evaluation Of Biochemical Parameters In Seminal Plasmamentioning

confidence: 99%

Some Heavy Metals Correlated Negatively with Total Antioxidant Capacity, Glutathione Peroxidase, Fructose, and Testosterone in Seminal Plasma of Oligospermic and Azoospermic Males

Umoinyang

Elekima

Onwuli

2021

JAMPS

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Aim: The study aimed to investigate the relationship between some heavy metals and total antioxidant capacities, glutathione levels, fructose, and testosterone in seminal plasma of infertile azoospermic and oligospermic males in Akwa Ibom State of Nigeria. Study Design: A cross-sectional design with a total of 124 males included in the study of which 32 were azoospermic, 38, oligospermic, and 54, normospermic. Place and Duration of Study: Semen samples were collected from the urology or fertility clinic of UUTH, St. Luke’s Hospital, Anua, and Ibom specialist hospital of Akwa Ibom State. However, laboratory assays were performed at the Department of Medical Laboratory Science, Rivers State University, Port Harcourt between May, 2018 and January, 2021. Methodology: Semen specimens were collected after 3-5 days abstinence according to WHO criteria while seminal plasma were obtained from semen by spinning at 4500 rpm for 10 minutes and stored at -700C prior to laboratory analysis. Atomic absorption spectrometer (AAS) was used to determine the levels of heavy metals while ELISA methods were used to determine testosterone and GSH concentrations. TAC and fructose assays were carried out using spectrophotometric methods. Results: Results showed that non-essential heavy metals such as lead, mercury, and arsenic correlated negatively with testosterone, fructose, and antioxidant activities of the seminal plasma in azoospermic subjects. In addition, lead and mercury correlated positively in the azoospermic subjects. Meanwhile, selenium, an essential heavy metal, correlated positively with testosterone and antioxidant activities in oligospermic subjects with 11-19 x106cells/ml. Conclusion: The levels of non-essential heavy metals in azoospermic subjects precipitated poor anti-oxidant and testosterone activities inducing oxidative stress while in oligospermic subjects, selenium and antioxidant parameters and testosterone were in good association indicating improved antioxidant activities and testicular function.

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“…1 mg/ ml) at 37°C. For incubations of isolated fat cells the medium described in Gliemann (1965) was used containing 50 nCi D-[1-14 C]glucose, final concentration 0.11 mg/ml. Reaction was stopped by addition of 0.5 ml H 2 SO 4 4 mol/l, and C0 2 was trapped according to Gliemann (1965) and counted in a Packard Tri-Carb liquid scintillation spectrometer.…”

Section: Isolation Of Fat Tissuesmentioning

confidence: 99%

“…Chromium was determined on a 240 mark 2 atomic absorption spectrophotometer (Evans electroselenium LTP, Halsted, England) with a hollow cathode lamp with an absorption optimum at 357.8 nm. Digestion was performed in a 1:1 mixture of nitric acid and hydrogen peroxide (Kayne, Komar, Laboda and Vauderlinde 1978) for 16 hours at 96°C. K,Cr,0, was used as a standard.…”

Section: Determination Of Chromiummentioning

confidence: 99%

On the Nature of the Glucose Tolerance Factor from Yeast

Urumow¹,

Wieland²

2008

Horm Metab Res

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The aim of this study was to purify the glucose tolerance factor (GFT) from yeast, and to gain further knowledge on its chemical structure. Activity of GTF was determined by its potency to stimulate 1-14C glucose oxidation of rat adipose tissue, in vitro, in the presence of maximally effective insulin concentrations. While described procedures Toepfer, Merth, Polansky, Roginski and Wolf (1977) resulted in some 3-fold purification, specific activity was no more increased by further fractionation on HPLC and/or TLC. Instead separation on TLC yielded several fractions with GTF activity, two of them enriched in aspartate and cystine/cystein, respectively, being the most important. L-aspartate, when added at comparable concentrations in vitro displayed similar GTF-activity as did adenosine, another component present in yeast extracts. It is concluded that GTF-activity of yeasts is not attributable to a single molecular species but rather to the combined action of amino acids and nucleosides especially aspartate, and adenosine.

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Atomic absorption spectrophotometry of chromium in serum and urine with a modified Perkin-Elmer 603 atomic absorption spectrophotometer.

Cited by 63 publications

References 0 publications

Metabolism and possible health effects of aluminum.

Metabolism and possible health effects of aluminum.

Some Heavy Metals Correlated Negatively with Total Antioxidant Capacity, Glutathione Peroxidase, Fructose, and Testosterone in Seminal Plasma of Oligospermic and Azoospermic Males

On the Nature of the Glucose Tolerance Factor from Yeast

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