Atomic Absorption Spectrometry and Scanning Electron Microscopy Evaluation of Concentration of Calcium Ions and Smear Layer Removal With Root Canal Chelators

Spanó, Júlio César Emboava; Silva, Ricardo Gariba; Guedes, Débora Fernandes Costa; Sousa‐Neto, Manoel Damião; Estrela, Carlos; Pécora, Jesus Djalma

doi:10.1016/j.joen.2009.02.008

Cited by 90 publications

(123 citation statements)

References 32 publications

(63 reference statements)

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“…For the effective removal of organic and inorganic components from the smear layer, irrigation with EDTA combined with different concentrations of sodium hypochlorite (NaOCl) has been recommended (4). However, the search for solutions more biocompatible than EDTA, in an attempt to minimize damage to the periapical tissues, has appeared with increasing frequency in the literature (5).…”

Section: Introductionmentioning

confidence: 99%

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Time-dependent effects of chitosan on dentin structures

et al. 2012

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Complete debridement with smear layer removal are essential measures for achieving a successful outcome of root canal treatment. The aim of this study was to evaluate the effects of chitosan at different concentrations on the removal of the smear layer and on dentin structure after 3 and 5 min of application. Twelve recently extracted maxillary canine teeth were instrumented using the crown-down technique and irrigated with 1% sodium hypochlorite. The specimens were distributed according to the time and concentration of the final irrigating solution: G1: 0.1% chitosan for 3 min; G2: 0.2% chitosan for 3 min; G3: 0.37% chitosan for 3 min; G4: 0.1% chitosan for 5 min; G5: 0.2% chitosan for 5 min; G6: 0.37% chitosan for 5 min. All samples were prepared for SEM analysis. G1 exhibited removal of the smear layer, but not the smear plugs. G2 showed visible and open tubules with slight erosion of the peritubular dentin. Cleaning in G3 was similar to that in G2, however, the erosive effect was greater. There was expansion of the diameter of the tubules in G4; and in G5 and G6, there was severe erosion with deterioration of dentin surface. In conclusion, 0.2% chitosan for 3 min appeared to be efficient for removing the smear layer, causing little erosion of dentin.

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Section: Introductionmentioning

confidence: 99%

“…Sen et al (7) observed erosion of the root dentin after using EDTA in concentrations of 1, 5, 10 and 15% for 1 min. Another disadvantage is that EDTA is considered a pollutant, since this substance is not found originally in nature (4).…”

Section: Introductionmentioning

confidence: 99%

Time-dependent effects of chitosan on dentin structures

et al. 2012

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“…Spano et al [17] showed using atomic absorption spectrophotometry that apple vinegar removed amounts of calcium ions from the root canal similar to 5% malic acid, and 5% acetic acid. These solutions were less effective than 10% citric acid and the EDTA resulted in the greatest concentration of calcium ions.…”

Section: Resultsmentioning

confidence: 99%

“…The efficiency of the chelating agents in the removal of the smear layer, demineralization and reduction of microhardness can be analyzed through various methods such as Atomic absorption spectrometry, scanning electron microscopy [17], digital optical microscopy [18] and microhardness tests [8][9][10]. The microhardness test is widely used to classify materials and compare changes in their properties, being a simple, effective and well defined The efficacy of a chelating agent is dependent on the time for which it remains in the root canal, since the solution does not act immediately when placed in contact with the dentin, requiring some minutes to obtain a chelating effect [24].…”

Section: Discussionmentioning

confidence: 99%

Avaliação in vitro do efeito de soluções de vinagre sobre a microdureza da dentina radicular

Falcão¹,

Pereira²,

Rebelo³

et al. 2014

Rev. Odonto Ciênc. (Online)

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Objective: This in vitro study evaluated the action of vinegar solutions on microhardness of root dentin. Methods: Ten extracted human maxillary central incisors were sectioned transversely at the cementenamel junction. The roots were fixed on cutting machine device and were cut on cervical region transversely in 2-mm sections. The second cervical section was divided into four parts. Each one quarter of section was fixed with wax in the center of a pre-fabricated acrylic disc. Four treatment groups were tested: group 1 -alcohol vinegar; group 2 -white wine vinegar; group 3 -EDTA (standard group); group 4 -distilled water (control group). The specimens were submitted to solutions application for 5 min. The measurements of microhardness (initial and post-treatment) were carried out using a Knoop microhardness tester. Data were analyzed by using Wilcoxon and Kruskal Wallis tests. Results: The analysis of initial and final microhardness showed statically significant differences before and after application of the tested solutions alcohol vinegar, white wine vinegar and EDTA. Groups 1,2 and 3 presented a decrease in the microhardness values. Conclusion: The solutions of alcohol vinegar and white wine vinegar, as well as the EDTA, reduced the microhardness of root dentin.

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“…In order to compensate for possible limitations in the root canal treatment resulting from the obstacles faced during preparation, a 2.5% sodium hypochlorite solution was used, with a greater concentration of active chlorine when compared with the concentration of 1% (2). Calcium hydroxide dressing was used to enhance disinfection of dentinal tubules (16,17). During root canal filling, it was necessary to customize a longer-than-usual master gutta-percha point.…”

Section: Discussionmentioning

confidence: 99%

Endodontic treatment of a 36-mm long upper cuspid: clinical case report

Barletta¹,

Grecca²,

Wagner³

et al. 2010

Rev. odonto ciênc.

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Purpose: To report a clinical case of a 36 mm long upper cuspid submitted to root canal treatment, with emphasis on the alternative technique employed.Case description: A 32 years old, male patient had suffered subluxation of teeth 12 and 13 and coronal fracture involving enamel and dentin, as well as pulp exposition, of tooth 14 because of a motorcycle accident. The man was given dental assistance and a semi-rigid splinting of teeth numbers 12 and 13 was carried out followed by the root canal treatment in a single visit on tooth no. 14. At 30-day follow-up pulp necrosis was detected in teeth 12 and 13 and the root canal treatment was implemented. The tooth 13 was 36 mm long and since the longest possible file (31 mm) was already in use, the cervical limit of the access cavity was considered as the reference point.Conclusion: This technique not only provided adequate disinfection, preparation and filling of the root canal, but it also allowed preservation of the remaining tooth structure.Key words: Anatomy; cuspid; dental pulp cavity; endodontics ResumoObjetivo: Descrever o tratamento endodôntico de um canino superior medindo 36 mm de comprimento, mostrando a técnica empregada no preparo do canal radicular e obturação.Descrição do Caso: Paciente do sexo masculino, com 32 anos de idade, sofreu subluxação dos dentes 12 e 13 e fratura coronária envolvendo esmalte e dentina, com exposição pulpar do dente 14, devido a um acidente de moto. No atendimento de urgência, foi realizada a contenção semirrígida dos dentes 12 e 13, seguida da endodontia em sessão única do dente 14. Trinta dias após, foi diagnosticada necrose pulpar dos dentes 12 e 13 e sua endodontia foi, então, iniciada. O comprimento aparente do dente 13 foi estabelecido em 36 mm e como o instrumento mais longo que dipomos no mercado possui 31 mm, o limite cervical da cavidade de acesso foi usado como bordo de referência.Conclusão: Além de garantir uma correta sanificação, modelagem e obturação do canal, esta técnica também permitiu a preservação da estrutura dental remanescente.

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Atomic Absorption Spectrometry and Scanning Electron Microscopy Evaluation of Concentration of Calcium Ions and Smear Layer Removal With Root Canal Chelators

Cited by 90 publications

References 32 publications

Time-dependent effects of chitosan on dentin structures

Time-dependent effects of chitosan on dentin structures

Avaliação in vitro do efeito de soluções de vinagre sobre a microdureza da dentina radicular

Endodontic treatment of a 36-mm long upper cuspid: clinical case report

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