Atomic Absorption Determination of Serum Copper: Collaborative Study

Abstract

Preliminary to conducting a collaborative study on a method for copper in serum, methods used by a selected group of laboratories were surveyed. The responding laboratories were supplied with a Youden pair of bovine serum samples and requested to use their current method for serum copper. Results of the analyses and the methods used were evaluated; hypotheses were developed in our laboratory to explain some of the interlaboratory variation. For the AAS method chosen, each of 12 collaborating laboratories analyzed one blind duplicate and 2 Youden pair of serum samples. A commercially available external control serum with a certified level of copper and a 1000 mg copper/L standard were also submitted. The method requires the serum to be diluted 1 + 1 with distilled water and the standards to be diluted with 10% glycerin to approximate the viscosity of the diluted serum. The intralaboratory coefficients of variation (CV) ranged from 2.24 to 4.40% and the interlaboratory CV ranged from 2.56% to 6.05%. The method has been adopted official first action.

Antioxidant status of Adult Nigerian Asthmatics: Implications for Prognosis

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Short Communication: Influence of Staphylococcus aureus Intramammary Infection on Serum Copper, Zinc, and Iron Concentrations

“…Milk SCC determinations were performed by the regional Dairy Herd Improvement Association laboratory (Mid-South Dairy Records) using an electronic counter. Serum Cu, Zn, and Fe concentrations were determined at the Veterinary Medical Diagnostic Laboratory (University of Missouri, Columbia) by atomic absorption spectrophotometry according to established guidelines (Osheim, 1983;Osheim and Ross, 1985).…”