AtMSI4 and RbAp48 WD‐40 repeat proteins bind metal ions<sup>1</sup>

Kenzior, Alexander; Folk, William R.

doi:10.1016/s0014-5793(98)01500-2

Cited by 31 publications

(27 citation statements)

References 35 publications

(33 reference statements)

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“…The Arabidopsis genome encodes five MSI1-like proteins Four MSI1-like genes, AtMSI1-4, were previously reported from Arabidopsis (Ach et al, 1997;Kenzior and Folk, 1998). After analysis of the complete Arabidopsis genome (The Arabidopsis Genome Initiative, 2000), we identified a fifth gene (At4g29730) encoding a protein similar to AtMSI1 that we termed AtMSI5.…”

Section: Resultsmentioning

confidence: 99%

“…Four MSI1-like proteins have been identified in Arabidopsis (AtMSI1-4), but only AtMSI1 is most similar to RbAp48 (Ach et al, 1997;Kenzior and Folk, 1998). Similar to MSI1-like proteins from mammals, Drosophila and yeast, AtMSI1 and its tomato homologue LeMSI1 are predominantly localised to the nucleus (Quian et al, 1993;Tyler et al,1996;Ach et al, 1997;Zhu et al, 2000;Bouché et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

et al. 2003

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WD40 repeat proteins similar to yeast MSI1 are conserved in animals and plants, in which they participate in complexes involved in chromatin metabolism. Although MSI1-like proteins are well characterised biochemically,their function in the development of multicellular eukaryotes is not well understood. We constructed Arabidopsis plants in which the AtMSI1 protein level was altered. Strong ectopic expression of AtMSI1 produced no visible altered phenotype, but reduction of AtMSI1 dramatically affected development. The primary shoot apical meristem was unable to develop organs after the transition to flowering. Flowers that developed on floral shoots from axillary meristems experienced a progressive loss of floral morphology,including a reduction in size of the petals and stamens and the development of carpel-like sepals. Ovule development was disrupted in all flowers, resulting in complete female sterility. Molecular analysis of the mutant plants revealed that AtMSI1 is required to maintain the correct temporal and organ-specific expression of homeotic genes, including AGAMOUS and APETALA2. In contrast, FAS1 and FAS2, which together with AtMSI1 form the chromatin assembly complex CAF-1, are not required for repression of these genes. Therefore, AtMSI1 has specific functions in addition to CAF-1-mediated chromatin assembly. Efficient formation of heterochromatin, but not methylation of centromeric DNA repeats, depends on AtMSI1 presence demonstrating a key role of AtMSI1 in maintenance of chromatin structure.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

et al. 2003

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“…One exception was a third E(z) homologue, GenBank accession At4g02020, that had not been characterised genetically. In addition, there are four genes with weak similarity to MSI1, MSI2-5, with poorly defined functions (Ach et al, 1997;Hennig et al, 2003;Kenzior and Folk, 1998). Thus, unlike Drosophila, in which the PRC2 members are single copy genes, in Arabidopsis the different members are mostly small gene families.…”

Section: Fertilisation-independent Endosperm (Fie) and Multicopy Suppmentioning

confidence: 99%

Interaction of Polycomb-group proteins controlling flowering inArabidopsis

et al. 2004

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In Arabidopsis, the EMBYRONIC FLOWER2 (EMF2), VERNALISATION2 (VRN2) and FERTILISATION INDEPENDENT ENDOSPERM2 (FIS2) genes encode related Polycomb-group (Pc-G)proteins. Their homologues in animals act together with other Pc-G proteins as part of a multimeric complex, Polycomb Repressive Complex 2 (PRC2), which functions as a histone methyltransferase. Despite similarities between the fis2 mutant phenotype and those of some other plant Pc-G members, it has remained unclear how the FIS2/EMF2/VRN2 class Pc-G genes interact with the others. We have identified a weak emf2 allele that reveals a novel phenotype with striking similarity to that of severe mutations in another Pc-G gene, CURLY LEAF (CLF), suggesting that the two genes may act in a common pathway. Consistent with this, we demonstrate that EMF2 and CLF interact genetically and that this reflects interaction of their protein products through two conserved motifs, the VEFS domain and the C5 domain. We show that the full function of CLF is masked by partial redundancy with a closely related gene, SWINGER(SWN), so that null clf mutants have a much less severe phenotype than emf2 mutants. Analysis in yeast further indicates a potential for the CLF and SWN proteins to interact with the other VEFS domain proteins VRN2 and FIS2. The functions of individual Pc-G members may therefore be broader than single mutant phenotypes reveal. We suggest that plants have Pc-G protein complexes similar to the Polycomb Repressive Complex2 (PRC2) of animals, but the duplication and subsequent diversification of components has given rise to different complexes with partially discrete functions.

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“…Arabidopsis has five MSI1-like genes, MSI1-MSI5 (Ach et al, 1997;Kenzior and Folk, 1998;Hennig et al, 2003). Arabidopsis MSI1 is essential for gametophyte and seed development, and is a member of the Fertilisation independent seed (FIS) complex, which is similar to the Drosophila Polycomb repressive complex PRC2 (Köhler et al, 2003a;Guitton et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Regulation of flowering time byArabidopsis MSI1

et al. 2006

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The transition to flowering is tightly controlled by endogenous programs and environmental signals. We found that MSI1 is a novel flowering-time gene in Arabidopsis. Both partially complemented msi1 mutants and MSI1 antisense plants were late flowering, whereas ectopic expression of MSI1 accelerated flowering. Physiological experiments revealed that MSI1 is similar to genes from the autonomous promotion of flowering pathway. Expression of most known flowering-time genes did not depend on MSI1, but the induction of SOC1 was delayed in partially complemented msi1 mutants. Delayed activation of SOC1 is often caused by increased expression of the floral repressor FLC. However, MSI1 function is independent of FLC. MSI1 is needed to establish epigenetic H3K4 di-methylation and H3K9 acetylation marks in SOC1 chromatin. The presence of these modifications correlates with the high levels of SOC1 expression that induce flowering in Arabidopsis. Together, the control of flowering time depends on epigenetic mechanisms for the correct expression of not only the floral repressor FLC, but also the floral activator SOC1.

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AtMSI4 and RbAp48 WD‐40 repeat proteins bind metal ions¹

Cited by 31 publications

References 35 publications

ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

Interaction of Polycomb-group proteins controlling flowering inArabidopsis

Regulation of flowering time byArabidopsis MSI1

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AtMSI4 and RbAp48 WD‐40 repeat proteins bind metal ions1

Cited by 31 publications

References 35 publications

ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

ArabidopsisMSI1 is required for epigenetic maintenance of reproductive development

Interaction of Polycomb-group proteins controlling flowering inArabidopsis

Regulation of flowering time byArabidopsis MSI1

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AtMSI4 and RbAp48 WD‐40 repeat proteins bind metal ions¹