Atmospheric Pressure Plasma Treatment with Nitrogen Induces Osteoblast Differentiation and Reduces iNOS and COX-2 Expressions

Satō, Ryōichi; Namura, Yasuhiro; Tanabe, Natsuko; Sakai, Michihiko; Utsu, Akihisa; Tomita, Keiko; Suzuki, Naoto; Motoyoshi, Mitsuru

doi:10.2485/jhtb.30.131

Cited by 1 publication

(1 citation statement)

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“…To determine whether NTAPP treatment (3 times, 20 s each) could promote the proliferation of hDDPFs without affecting pluripotency, we examined the expression of Oct4, Sox2, and Nanog. We also studied the expression of Sox9, a marker of pluripotency of undifferentiated neural crest cells, and ALP, a marker of pluripotency of undifferentiated osteoblasts since hDDPFs are known to differentiate into chondrocytes and osteoblasts [27][28][29]. Our results showed that mRNA expression of Oct3/4, Sox2 and Nanog was upregulated in NTAPPtreated cells.…”

Section: Discussionmentioning

confidence: 95%

Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells

et al. 2021

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Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state; however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., Oct4, Sox2, and Nanog), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications.

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Section: Discussionmentioning

confidence: 95%