Atlas of the Neonatal Rat Brain

Ramachandra, Renuka; Subramanian, Thyagarajan

doi:10.1201/b10500

Cited by 43 publications

(38 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regional boundaries in neonate hippocampi were determined by cross-referencing with the atlases of neonatal rat brain (Paxinos and Watson, 1998;Ramachandra and Subramanian, 2016). Regional boundaries in neonate hippocampi were determined by cross-referencing with the atlases of neonatal rat brain (Paxinos and Watson, 1998;Ramachandra and Subramanian, 2016).…”

Section: Quantification Analysismentioning

confidence: 99%

WITHDRAWN: Developmental regulation and lateralization of GABA receptors in the rat hippocampus

Khoshdel‐Sarkarizi¹,

Hami²,

Mohammadipour³

et al. 2019

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Section: Quantification Analysismentioning

confidence: 99%

WITHDRAWN: Developmental regulation and lateralization of GABA receptors in the rat hippocampus

Khoshdel‐Sarkarizi¹,

Hami²,

Mohammadipour³

et al. 2019

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

“…For the sectioning of neonatal rat brains whole heads were used and the sectioning was guided by the neonatal rat brain atlas (Ramachandra and Subramanian, 2011). Slides were stored at -80 C until use.…”

Section: In Situ Hybridization Histochemistry (Ishh)mentioning

confidence: 99%

5-HT2A and 5-HT2C receptors as hypothalamic targets of developmental programming in male rats

et al. 2016

View full text Add to dashboard Cite

List of abbreviations5As the prevalence of obesity increases across the globe major efforts are directed at studying the mechanisms involved. Growing evidence suggests that susceptibility to obesity can be programmed by maternal and neonatal nutrition. In particular, individuals with low birth weight that undergo rapid postnatal growth are at higher risk of developing increased adiposity in later life. The molecular mechanisms mediating the interaction between early nutrition and obesity risk are, however, still largely unknown. Currently, there are no pharmacological therapies to prevent the increase in adiposity in individuals that underwent nutritional challenge in early life. Serotonin (5-HT) system is widely recognized as one of the key regulators of food intake and body weight. During fetal life serotonin also acts as a trophic factor that regulates brain development. As compounds targeting endogenous serotonin bioavailability have been among the most clinically effective treatments for obesity (e.g. d-fenfluramine), treatment targeting serotonin system may prevent the development of obesity in at risk individuals. Results:The study has shown that maternal protein restriction increased levels of serotonin in the blood of pregnant rat mums and in the placenta and the fetal brain. In adulthood the offspring had a reduced ability to sense serotonin, which was associated with a reduced hypothalamic expression of 5-HT2Creceptors -the primary serotonin receptor influencing appetite. As expected, reduced 5-HT2Creceptor expression was associated with impaired sensitivity to serotonin-mediated appetite suppression and hence could lead to increases in food intake. On the other hand offspring of low protein dams had a programmed increase in 5-HT2A receptors, which are positioned, just like 5-HT2C receptors, in the arcuate nucleus of the hypothalamus -a key area of the brain, involved in the control of food intake. Moreover, these animals were more sensitive to 5-HT2A receptor agonistinduced appetite suppression. Implications and future directions:This study identified a molecular mechanism that is involved in mediating the effects of suboptimal maternal diet on the regulation of food intake in the offspring. In addition, the authors showed that a treatment with 5-HT2A receptor agonist may potentially prevent the development of obesity in individuals that were exposed in utero to suboptimal nutrition and then underwent rapid postnatal growth. Non-hallucinogenic 5-HT2A receptor agonist should be used for such treatment. Combining the 5-HT2A receptor agonist with 5-HT2CR and 5-HT1BR agonists could potentially further increase the anorectic therapeutic effect. Disease Models & Mechanisms • DMM • Advance article AbstractThough obesity is a global epidemic, the physiological mechanisms involved are little understood.Recent advances reveal that susceptibility to obesity can be programmed by maternal and neonatal nutrition. Specifically, a maternal low protein diet during pregnancy causes decreased intrauterine growth, rapid...

show abstract

“…Initially, for each animal, an average of 3–4 representative coronal sections containing the VTA area [46,47] were photographed bilaterally under 10x magnification at 400 ms exposure (Figure 1). Complementary sets of brain tissue per age were simultaneously processed for immunofluorescent labeling and were subsequently photographed with the same exposure time to minimize inter-assay variability.…”

Section: Methodsmentioning

confidence: 99%

“…The adult panel (A) corresponds to a plate 44 of the adult rat brain atlas [46], whereas neonatal panel (B) corresponds to a coronal plate 17 (viz. Figure 61) of the neonatal rat brain atlas [47]. The number in the upper right corner of the adult rat coronal section indicates the distance from Bregma.…”

Section: Figurementioning

confidence: 99%

Astrocytic hypertrophy in the rat ventral tegmental area following chronic morphine differs with age

Goins¹,

Bajic²

2018

neurology-neurorehabilitation

View full text Add to dashboard Cite

The ventral tegmental area (VTA) is the origin of the mesolimbic dopaminergic system known to play an integral role in mediating reward and development of drug addiction. Although the differences in neuronal plasticity of VTA at various ages remain to be understood, age is known to influence the effects of chronic opioids. In addition, adaptations associated with exposure to opioids within glial populations located in the VTA are poorly understood. The objective of the study was to determine if there are changes in astrocytic immunofluorescent labeling in the VTA following chronic morphine administration in a rat model at different ages: newborn at postnatal day (PD)7 and adult (estimated PD57). We hypothesized that increased immunohistochemical labeling of an astrocytic marker, glial fibrillary acidic protein (GFAP) in the VTA following chronic administration of morphine will not differ with age. Two groups of rats were analyzed: chronic morphine and saline control treatment groups. Either morphine (10 mg/kg) or equal volume of saline was given subcutaneously twice daily for 6½ days. On the 7th day of treatment, animals were anesthetized and perfused at one hour after the final drug injection. Coronal sections of the midbrain were processed for immunofluorescent identification of GFAP that was noted at both ages. We report an increase in both (1) GFAP labeling intensity, as well as (2) the percent area occupied by astrocytes that are immunoreactive for GFAP following chronic morphine when compared to saline treatment in the VTA only for the adults (n=6/group) but not infant rats at PD7 (n=5/group). Our findings suggest that adaptations in the mesolimbic dopaminergic system produced by repeated exposure to opioids may be associated with changes in glial function that differ with age.

show abstract

Atlas of the Neonatal Rat Brain

Cited by 43 publications

References 0 publications

WITHDRAWN: Developmental regulation and lateralization of GABA receptors in the rat hippocampus

WITHDRAWN: Developmental regulation and lateralization of GABA receptors in the rat hippocampus

5-HT2A and 5-HT2C receptors as hypothalamic targets of developmental programming in male rats

Astrocytic hypertrophy in the rat ventral tegmental area following chronic morphine differs with age

Contact Info

Product

Resources

About