Atlantic salmon ISG15: Expression and conjugation to cellular proteins in response to interferon, double-stranded RNA and virus infections

Røkenes, Torunn P.; Larsen, Rannveig; Robertsen, Børre

doi:10.1016/j.molimm.2006.03.016

Cited by 80 publications

(61 citation statements)

References 40 publications

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“…As expected, many cellular genes homologous to mammalian ISGs were retrieved from this cell system by suppressive subtractive hybridization, including TLR3, RIG-I, mda5, IFN, STAT1, IRF7, Mx1, Mx2, IFI56, IFI58, ISG15, PKR-like, Gig1 and Gig2 [20,21]. In addition, ISG15 homologues have also been discovered in goldfish [22], Atlantic salmon [23], and more recently in Atlantic cod [24], but only one copy of ISG15 gene was found in these species. In this study, we further determined the full-length nucleotide and amino acid sequences of two crucian carp ISG15 genes, and their differential kinetics in CAB cells under distinct stimuli, including virus infection, IFN containing supernatant (ICS), Poly I:C and LPS treatment.…”

Section: Introductionsupporting

confidence: 69%

“…However, in all species in which ISG15 has been identified, only one copy has been found. In fish, one copy of ISG15 gene was cloned in goldfish [22] and Atlantic salmon [23], which was further evidenced by Northern blot assay of Atlantic salmon kidney and cultured kidney cells where a single transcript of ISG15 gene was detected [23]. In the current study, two crucian carp ISG15 genes displayed a comparative divergence in nucleotide and amino acid sequences, so it is plausible that they are not generated as a result of different splicing from one gene.…”

Section: Discussionmentioning

confidence: 61%

“…One is lack of the Cys residues in the linker sequences of fish proteins, which is conserved in mammalian proteins and is essential for the stabilization of ISG15 [6]; the other is that fish proteins, together with ruminant ISG15 proteins, have not any additional C-terminal amino acids following the highly conserved ISG15 C-terminal sequence ''LRLRGG'' [2,23]. Like ubiquitin, ISG15 can be conjugated to target proteins via its C-terminal end [7].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the existence of structural differences, fish ISG15 were able to conjunct cellular proteins and a 65 kDa ISAV protein [23]. In fact, proteomic analysis has recently identified more than 150 mammalian protein targets of ISGylation including some IFN induced proteins, STAT1, Mx, IFI56, PKR and RIG-I [11,12].…”

Section: Discussionmentioning

confidence: 99%

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Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp

Zhang

Wang

Gui

2007

Fish & Shellfish Immunology

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Section: Introductionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp

Zhang

Wang

Gui

2007

Fish & Shellfish Immunology

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“…A number of genes related to the IFN system have been recently identified from different fish species, including the TLRs, the RIG-Ilike receptors, the TRIF and MAVS adapters, the IRF members, the JAK/STAT family members, and several IFN-stimulated genes (ISGs), such as protein kinase containing Z-DNA binding domains (PKZ) (35,36), ISG15 (37,38), CD40 (39), GTPase Mx protein (40), and viperin (cig1) (41). Overall, these are homologous to genes seen in other vertebrate species.…”

mentioning

confidence: 99%

Characterization of a PIAS4 Homologue from Zebrafish: Insights into Its Conserved Negative Regulatory Mechanism in the TRIF, MAVS, and IFN Signaling Pathways during Vertebrate Evolution

Xiong

Nie

Xiang

et al. 2012

The Journal of Immunology

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Members of the protein inhibitor of activated STAT (PIAS) family are key regulators of various human and mammalian signaling pathways, but data on their occurrence and functions in ancient vertebrates are limited. This study characterizes for the first time to our knowledge a PIAS4 homologue (PIAS4a) from zebrafish. Structurally, this zebrafish PIAS4a (zfPIAS4a) shares a number of conserved functional domains with mammalian PIAS4 proteins, including the scaffold attachment factor A/B/acinus/PIAS box, PINIT, and RING-finger–like zinc-binding domains and a highly acidic domain in the C-terminal region. Subcellular localization analysis shows that zfPIAS4a is a nuclear-localized protein and that the C terminus of the molecule harbors strict nuclear localization signals. Functionally, zfPIAS4a expression can be dramatically induced by the stimulation of polyinosinic-polycytidylic acid and zebrafish IFNϕ1. It acts as a critical negative regulator of the TIR domain-containing adapter inducing IFN-β, mitochondrial antiviral signaling (MAVS), and IFN signaling pathways, and it is the first PIAS protein that plays a role in the MAVS-mediated pathway to be identified. The structure and functionality of PIAS4 seem highly conserved from zebrafish to mammals, making zebrafish an attractive model for screens designed to uncover genes involved in IFN- and inflammatory cytokine-induced signaling pathways. This study provides preliminary evidence that the PIAS regulatory mechanism already existed in fish during vertebrate evolution. It presents valuable clues for improving the understanding of not only the negative regulation of cytokine signaling in fish but also the evolutionary history of the PIAS family from fish to mammals as a whole.

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Phenotype gene expression differences between resistant and susceptible salmon families to IPNV

Cofre

González

Moya³

et al. 2013

Fish Physiol Biochem

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Infectious pancreatic necrosis virus (IPNV) is an economically important pathogen of the salmonid aquaculture industry. Selective breeding has been employed to improve resistance to this infectious disease, and it is of importance to investigate the expression profile of immune genes of Atlantic salmon with different genetic background in response to this virus. This study examined the immune modulation response of eight candidate genes in head kidney tissue in two families of Atlantic salmon with high and low mortalities, after challenge with IPNV. The results showed that the expression pattern of target genes differed in the two families. Generally, higher expression of antiviral, pro-inflammatory genes and transcription factors such as tripartite motif, NF-κB, IFNI, STAT1, protein kinase R, and Vig-2 in the resistant family were observed at the same time point. One may speculate the functional importance of these putative candidate genes in the characterization of the IPNV-resistant (low mortalities) immune phenotype. Therefore, on our findings, we suggest that future salmonids studies aiming to identify candidate genes/pathway or vaccines evaluation should consider validating detected genes/pathway across different genetic backgrounds or immune phenotype.

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Atlantic salmon ISG15: Expression and conjugation to cellular proteins in response to interferon, double-stranded RNA and virus infections

Cited by 80 publications

References 40 publications

Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp

Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp

Characterization of a PIAS4 Homologue from Zebrafish: Insights into Its Conserved Negative Regulatory Mechanism in the TRIF, MAVS, and IFN Signaling Pathways during Vertebrate Evolution

Phenotype gene expression differences between resistant and susceptible salmon families to IPNV

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