Atividade Toxica Do Óleo Essencial De Piper Duckei (Piperaceae) Sobre Microcrustáceo Artemia Salina / Toxic Activity of Essential Oil of Piper Duckei (Piperaceae) on Microcrustacean Artemia Salina

Góes, Darlen Farias; Araújo, Jéssica Moreira; Oliveira, Ney Ricardo de Souza; Lima, Rodrigo Queiroz de

doi:10.34117/bjdv6n12-207

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“…Ten larvae of A. salina in the meta-nauplius stage were placed in each tube. After 24 h, the mortality rate of the larvae was quantified, and the mean lethal concentration (LC 50 ) was calculated using the Probitos statistical method, according to the methodology adapted from Góes et al [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

“…The radical inhibition activity (AIR) of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS •+ ) was analyzed according to the initial principles proposed by Miller et al [ 42 ] with the reaction conditions modified by Re et al [ 43 ]. The method is based on the ability of substances to eliminate the cationic ABTS •+ radical, a blue-green chromophore with maximum absorption at 734 nm, resulting in the formation of the stable, colorless ABTS product.…”

Section: Methodsmentioning

confidence: 99%

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Phytochemical Profile, Preliminary Toxicity, and Antioxidant Capacity of the Essential Oils of Myrciaria floribunda (H. West ex Willd.) O. Berg. and Myrcia sylvatica (G. Mey) DC. (Myrtaceae)

et al. 2022

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The essential oils (EOs) of Myrciaria floribunda (Mflo) and Myrcia sylvatica (Msyl) (Myrtaceae) were obtained by hydrodistillation. The analysis of volatile constituents was performed by GC/MS. Preliminary toxicity was assessed on Artemia salina Leach. The antioxidant capacity was measured by the ABTS•+ and DPPH• radical inhibitory activities. The results indicate that the Mflo EO had the highest yield (1.02%), and its chemical profile was characterized by high levels of hydrocarbon (65.83%) and oxygenated (25.74%) monoterpenes, especially 1,8-cineole (23.30%), terpinolene (22.23%) and α-phellandrene (22.19%). Regarding the Msyl EO, only hydrocarbon (51.60%) and oxygenated (46.52%) sesquiterpenes were identified in the sample, with (Z)-α-trans-bergamotene (24.57%), α-sinensal (13.44%), and (Z)-α-bisabolene (8.33%) at higher levels. The EO of Mflo exhibited moderate toxicity against A. salina (LC50 = 82.96 ± 5.20 µg.mL−1), while the EO of Msyl was classified as highly toxic (LC50 = 2.74 ± 0.50 µg.mL−1). In addition, relative to Trolox, the EOs of Mflo and Msyl showed significant inhibitory effects (p < 0.0001) against the DPPH• radical. This study contributes to the expansion of chemical and biological knowledge on the EOs of Myrtaceae species from the Amazon region.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%