Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Oliveira, Leonardo Bruno Sampaio de; Batista, A.H.M.; Fernandes, F.C.; Sales, Gleilton Weyne Passos; Nogueira, Nádia Accioly Pinto

doi:10.1590/1983-084x/15_222

Cited by 25 publications

(16 citation statements)

References 20 publications

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“…The microplate was incubated for 24 to 48 h in an incubator at 37 °C and resazurin was added to determine microbial growth 53 . The MIC for yeast was determined by a modified broth microdilution method 54 .…”

Section: Methodsmentioning

confidence: 99%

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Figueiredo

Filho

Sodré

et al. 2021

Sci Rep

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In the present study, novel, 1,3-diaryltriazene-derived triazene compounds were synthesized and tested. Triazenes are versatile and belong to a group of alkylating agents with interesting physicochemical properties and proven biological activities. This study describes the synthesis, molecular and crystalline structure, biological activity evaluation, and antifungal and antimicrobial potentials of 1,3-bis(X-methoxy-Y-nitrophenyl)triazenes [X = 2 and 5; Y = 4 and 5]. The antimicrobial and antifungal activities of the compounds were tested by evaluating the sensitivity of bacteria (American Type Culture Collection, ATCC) and clinical isolates to their solutions using standardized microbiological assays, cytotoxicity evaluation, and ecotoxicity tests. The antimicrobial potentials of triazenes were determined according to their minimum inhibitory concentrations (MICs); these compounds were active against gram-positive and gram-negative bacteria, with low MIC values. The most surprising result was obtained for T3 having the effective MIC of 9.937 µg/mL and antifungal activity against Candida albicans ATCC 90028, C. parapsilosis ATCC 22019, and C. tropicallis IC. To the best of our knowledge, this study is the first to report promising activities of triazene compounds against yeast and filamentous fungi. The results showed the potential utility of triazenes as agents affecting selected resistant bacterial and fungal strains.

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Section: Methodsmentioning

confidence: 99%

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Figueiredo

Filho

Sodré

et al. 2021

Sci Rep

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“…Brito et al (2015), showed that the C. krusei and C. tropicalis strains cultivated in culture medium in the presence of the Lippia sidoides (Verbenaceae) essential oil did not present pseudohyphae and hyphae at the 256 μg/mL concentration. Meanwhile, Oliveira, Batista, Fernandes, Sales, and Nogueira (2016) showed a reduction in C. albicans hyphae at the 1.25 mg/mL and 2.5 mg/mL concentrations, as well as C. albicans (0.625 mg/mL and 2.5 mg/mL) and C. parapsilosis (1.25 mg/mL and 2.5 mg/mL) treated with the Ocimum gratissimum (Lamiaceae) essential oil.…”

Section: Antifungal Activitymentioning

confidence: 99%

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

et al. 2018

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The development of fungal resistance to antifungal drugs has been worsening over the years and as a result research on new antifungal agents derived from plants has intensified. Eugenia uniflora L. (pitanga) has been studied for its various biological actions. In this study the chemical composition and antifungal effects of the E. uniflora essential oil (EULEO) were investigated against Candida albicans (CA), Candida krusei (CK) and Candida tropicalis (CT) standard strains. The essential oil obtained through hydro-distillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). To determine the IC of the oil, the cellular viability curve and the inhibitory effects were measured by means of the oil's association with Fluconazole in a broth microdilution assay with spectrophotometric readings. The Minimum Fungicidal Concentration (MFC) was determined by solid medium subculture with the aid of a guide plate while the assays used to verify morphological changes emerging from the action of the fractions were performed in microculture chambers at concentrations based on the microdilution. Two major oil constituents stand out from the chemical analysis: selina-1,3,7(11)-trien-8-one (36.37%) and selina-1,3,7(11)-trien-8-one epoxide (27.32%). The concentration that reduced microorganismal growth was ≥8,192 μg/mL while the IC varied, this being between 1892.47 and 12491.80 μg/mL (oil), 10.07 - 80.78 μg/mL (fluconazole) and 18.53 - 295.60 μg/mL (fluconazole + oil). The combined activity (fluconazole + oil) resulted in indifference and antagonism. A MFC of the oil in association with fluconazole was recorded at the concentration of 8,192 μg/mL against CA and CK. The oil caused the inhibition of CA and CT morphological transition. In view of the results obtained, additional research is needed to elucidate the activity of the E. uniflora oil over genetic and biochemical processes regarding its effect on Candida spp. virulence.

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“…L. monocytogenes is known to cause the disease listeriosis, which affects principally immunocompromised patients, thereby having a mortality rate of 30%. Furthermore, the bacteria bear the ability for biofilm formation, and by this way developing resistance to sanitizers and antimicrobial agents, thus provoking serious problems to food industries (Oliveira et al, 2016b;Rogalla and Bomar, 2018;Marini et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

et al. 2021

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The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.

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Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Cited by 25 publications

References 20 publications

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

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