ATIVIDADE ANTIBIOFILME DE DITERPENO ISOLADO DE Croton antisyphiliticus FRENTE Staphylococcus aureus.

Abstract

RESUMOO presente estudo teve como principal objetivo avaliar o potencial de atividade antimicrobiana de diterpeno isolado de Croton antisyphiliticus, espécie nativa do Cerrado, frente biofilme de Staphylococcus aureus, isolados do leite de animais com mastite. O diterpeno ácido ent-kaur-16-en-18-óico foi avaliado quanto à capacidade de erradicação da biomassa e também à redução da população bacteriana sob o biofilme. O antibiótico sulfato de gentamicina foi utilizado como tratamento comparativo. A atividade antibiofilme foi determinada por meio dos métodos do Cristal Violeta e da Contagem de Unidades Formadoras de Colônia. Os resultados obtidos demonstraram que o ácido ent-kaur-16-en-18-óico, na concentração de 250 µg/mL, reduziu 56% da biomassa formada, enquanto a gentamicina, na concentração de 30 mg/mL, apenas 13%. Quanto à atividade nas células bacterianas sob o biofilme, não houve diferença estatística significativa entre os tratamentos, que minimizaram cerca de 3 logs da população de S. aureus. Portanto, nas condições ensaiadas, o diterpeno ent-kaur-16-en-18-óico destacou-se por apresentar atividade antibiofilme significativa contra S. aureus, em concentração 120 vezes menor quando comparado ao antibiótico gentamicina. PALAVRAS-CHAVE:Ácido ent-kaurenoic. Atividade antimicrobiana. Biofilme. Mastite. Croton SUMMARYThe present study aimed to evaluate the potential antimicrobial activity of diterpene isolated from Croton antisyphiliticus, native species of the Cerrado, against biofilm of Staphylococcus aureus isolated from milk from animals with mastitis. The diterpene ent-Kaur-16-on-18-oic acid were evaluated for their ability to eradicate biomass and also reducing the bacterial population in the biofilm. The antibiotic gentamicin was used as a comparative treatment. Antibiofilm activity was determined by the methods of Crystal Violet and Count of Colony Forming Units. The results demonstrated that the ent-Kaur-16-on-18-oic acid, at a concentration of 250 μg/mL, reduced 56% of the biomass formed, while gentamicin, at a concentration of 30 mg/ml, only 13%. Regarding the activity in bacterial cells in the biofilm, there was no statistically significant difference between treatments, that minimized about 3 logs of the population of S.aureus. Therefore, in the conditions tested, the diterpene ent-kaur-16-on-18-oic highlighted for presenting antibiofilm significant activity against S. aureus, at a concentration 120 times lower when compared to the gentamicin antibiotic.

Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

“…(2013) reported that tannins from medicinal plant may prevent the formation of biofilms. The diterpene isolated from the Croton antisyphiliticus root chloroform extract was not able to completely reduce biofilms, but efficient inhibitory activity was found, capable of promoting significant antibiofilm activity by eliminating 56% S. aureus at 250 µg/ml (Nader et al., 2014).…”

“…The disinfectants and antibiotics ineffectiveness have been related to bacterial biofilm formation in food industry (Mafu et al., 2011; Simões et al., 2010), conducting pathogens to consumers and causing outbreaks of foodborne illnesses (Chmielewski and Frank, 2003). Bacterial cells in biofilms can be up to 1000 times more resistant to antibiotics and sanitizing agents (Nader et al., 2014). Due to the difficulties to reduce cells adhered to surfaces, new strategies for the biofilm control have been studied (Donlan, 2002), such as plants containing flavonoids that may break outer membrane and inhibit bacteria enzymatic activity (Nascimento et al., 2000).…”

Effect of Mauritia flexuosa L. leaf extract on Staphylococcus aureus and Staphylococcus haemolyticus biofilms adhered to stainless steel surface

“…The ineffectiveness of disinfectants and antibiotics has been frequently related to bacterial biofilms (Mafu et al, 2011), because the biofilms cells are up to 1,000 times more resistant to these antimicrobial agents (Nader et al, 2014). Due to the difficulties in reducing bacteria in biofilms, new control strategies have been evaluated (Donlan, 2002).…”