Atividade antiaderente dos óleos essenciais de Eucalyptus globulus e Eucalyptus citriodora contra cepas de Klebsiella pneumoniae

Abstract

The present work aims to evaluate the Minimum Inhibitory Concentration of Adherence (MICA) of the essential oils of Eucalyptus globulus and Eucalyptus citriodora against the bacterium Klebsiella pneumoniae. It was performed the determination of the Minimum Inhibitory Concentration of Adherence (MICA) using the inclined tube technique, in the presence of 5% sucrose. After 24 hours, the reading was performed through visual observation of the bacterium's adherence to the tube walls. It was observed that the essential oil of Eucalyptus globulus showed a good antiadherent efficiency to the strain of Klebsiella pneumoniae, showing biofilm inhibition at a concentration of 1:8 similar to the 0.12% chlorhexidine digluconate control. Eucalyptus citriodora oil did not inhibit biofilm formation in any of the tested concentrations. It was concluded that only the essential oil belonging to the Eucalyptus globulus species, showed antiadherent action against the strain of Klebsiella pneumoniae, which can be used as an alternative method to combat biofilms produced by K. pneumoniae.

Evaluation of the antimicrobial activity of Eucalyptus radiata essential oil against Escherichia coli strains isolated from meat products

“…However, in the present research, the product being tested in this study was effective in inhibiting the adherence of Escherichia coli. This corroborates the findings of Ramalho et al (2020), who observed that the essential oil of Eucalyptus globulus had an anti-adherent activity of 1:64, showing a positive result at a lower concentration of the oil compared to the result obtained by chlorhexidine digluconate 0.12%, which showed inhibition at a higher concentration of 1:8, confirming that this plant species is also effective in inhibiting biofilm formation against clinical strains of E. coli of human origin.…”

Evaluation of the antimicrobial effect of the Origanum vulgare L essential oil on strains of Klebsiella pneumoniae

“…Moreover, taking into account the bacterial strain, Ramalho et al (2020), used strains of Klebsiella pneumoniae (kp 102), grown in Mueller Hinton broth in test tubes in order to evaluate the Minimum Inhibitory Concentration of Adherence (MIAC) of the essential oils of Eucalyptus globulus and Eucalyptus citriodora, and as a positive control was employed chlorhexidine digluconate at 0.12%. Thus, when analyzing the results of the anti-adherent activity of the EOs, it was obtained that the EO of Eucalyptus globulus showed good efficiency against biofilm formation by Klebsiella pneumoniae showing results equivalent to the control chlorhexidine digluconate 0.12%, and inhibiting biofilm formation at the same concentration 1:8.…”

Gestão: O Remédio Que a Saúde Necessita

“…O método da CIMA foi utilizado na presente pesquisa por permitir avaliar o potencial antiaderente dos produtos naturais utilizados no estudo de forma rápida e eficaz. Além disso, outra vantagem desse método se caracteriza pela análise experimental de forma observacional direta, possibilitando a seleção de produtos com potencial antiaderente (RAMALHO et al 2020). Fonte: Autoria própria.…”