Atherosclerosis: perspectives of anti-inflammatory therapy

Насонов, Е Л; Попкова, Т. В.

doi:10.26442/terarkh20189054-12

Cited by 17 publications

(10 citation statements)

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“…The inflammatory response of the vascular endothelium plays a major role in the occurrence and development of AS. The rolling, adhesion, migration, and accumulation of white blood cells are the features of the inflammatory response in the vascular endothelium [ 32 ]. Under the stimulation of external environmental factors, endothelial cells can be stimulated to produce a variety of inflammatory factors and adhesion molecules, accelerate the adhesion of white blood cells to endothelial cells, and promote the occurrence of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway

Liu

Yan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to explore the effects of shikonin (SKN) on the damage of human venous endothelial cells (HUVECs) induced by ox-LDL and the underlying molecular mechanism. The HUVECs were randomly divided into six groups: control, ox-LDL, SKN + ox-LDL, SKN + ox-LDL + compound C, SKN + ox-LDL + si-Nrf2, and SKN + ox-LDL + si-HO-1. The MTT method was used to detect cell viability, flow cytometry was used to detect cell apoptosis and reactive oxygen species (ROS) levels, and Western blot was used to detect protein levels. Compared to the control group, the cell viability of the ox-LDL group decreased, the apoptosis rate increased, the level of cleaved caspase-3 was upregulated, and the level of Bcl-2 protein was downregulated. The level of TNF-α, IL-1β, IL-6, vascular cell adhesion molecule-1 (VCAM1), intercellular adhesion molecule-1 (ICAM1), and E-selectin (E-sel) was increased, ROS levels increased, and superoxide dismutase (SOD) level decreased. Moreover, the protein levels of p-AMPK, Nrf2, and HO-1 were decreased. Compared to the ox-LDL group, SKN treatment improves cell viability, alleviates cell apoptosis and oxidative stress injury, and upregulates the protein levels of p-AMPK, Nrf2, and HO-1. Compound C, si-Nrf2, and si-HO-1 administration inhibits the AMPK/Nrf2/HO-1 signaling pathway, increases ROS generation, and inhibits the antagonistic effect of SKN on ox-LDL-induced HUVECs damage. In summary, SKN suppressed ox-LDL-induced ROS production and improved cell viability and cell apoptosis via the AMPK/Nrf2/HO-1 pathway.

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Section: Discussionmentioning

confidence: 99%

Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway

Liu

Yan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The inflammasome has been identified as a multi-protein complex which plays a key role in innate immune responses against pathogens and toxic metabolites (Nakanishi et al, 2018). Among those types of inflammasomes, NOD-like receptor pyrin domain containing 3 (NLRP3) possesses a critical role in inflammatory response (Li et al, 2018), and associated with many diseases, including AD, Parkinson's disease, diabetes, atherosclerosis, and cerebral ischemia/reperfusion injuries (Guo et al, 2016;Feng et al, 2018;Li et al, 2019;Nasonov and Popkova, 2018;von Herrmann et al, 2018). NLRP3 activation induces aggregation of ASC, leading to caspase-1 activation, promoting the release of inflammatory factors (Stancu et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Yang

Huang

et al. 2020

Front. Pharmacol.

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This present study was designed to investigate the different effects of ginsenosides Rb1 and Rg1 on improving cognitive deficits in 4-month-old SAMP8 mice. Mice were divided into six groups, including the SAMP8 group, the SAMP8 + Donepezil (1.6 mg/kg) group, the SAMP8 + Rb1 (30 and 60 µmol/kg), and SAMP8 + Rg1 (30 and 60 µmol/kg) groups. SAMR1 mice of the same age were used as the control group. Ginsenosides and donepezil were administrated orally to animals for 8 weeks, then the learning and memory ability of mice were measured by using Morris water maze (MWM) test, object recognition test and passive avoidance experiments. The possible mechanisms were studied including the anti-glial inflammation of Rb1 and Rg1 using HE staining, immunohistochemistry and western blot experiments. Results revealed that Rb1 and Rg1 treatment significantly improved the discrimination index of SAMP8 mice in the object recognition test. Rb1 (60 µmol/kg) and Rg1 (30, 60 µmol/kg) could significantly shorten the escape latency in the acquisition test of the MWM test in SAMP8 mice. Furthermore, Rb1 and Rg1 treatments effectively reduced the number of errors in the passive avoidance task in SAMP8 mice. Western blot experiments revealed that Rb1 showed higher effect than Rg1 in decreasing protein expression levels of ASC, caspase-1 and Ab in the hippocampus of SAMP8 mice, while Rg1 was more effective than Rb1 in decreasing the protein levels of iNOS. In addition, although Rb1 and Rg1 treatments showed significant protective effects in repairing neuronal cells loss and inhibiting the activation of astrocyte and microglia in hippocampus of SAMP8 mice, Rb1 was more effective than Rg1. These results suggest that Rb1 and Rg1 could improve the cognitive impairment in SAMP8 mice, and they have different mechanisms for the treatment of Alzheimer's disease.

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“… 11 Moreover, CLGI played a crucial role in all the stages of AS formation, implying that anti-inflammatory therapy was of great significance for the treatment of atherosclerosis-related cardiovascular diseases. 12 Similarly, NAFLD was also associated with CLGI, and closely related to macrophage-mediated inflammation. 13 Studies have demonstrated that adiponectin levels in MIS patients tend to be lower compared to those in non-MIS patients, indicating that adiponectin could be used as an indicator to predict the occurrence of MIS.…”

Section: Discussionmentioning

confidence: 98%

Serum Heparanase: A New Clinical Biomarker Involved in Senile Metabolic Inflammatory Syndrome

Wang¹,

Zhou²,

Mei³

et al. 2021

DMSO

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Aim: Metabolic inflammation syndrome (MIS) can lead to a series of complications, but its exact inflammatory mechanism is still unclear. The aim of this study was to explore the correlation between heparanase (HPA) and MIS, and the close relationship between HPA and other chronic low-grade inflammation index, such as C-reactive protein (CRP) and interleukin-6 (IL-6). Methods: A total of 105 patients with MIS in the physical examination population of Huashan Hospital affiliated to Fudan University from May to June 2018 were selected as the MIS group, and 52 patients who were relatively healthy during the same period were used as the control group. The basic clinical data of the selected candidates were collected, the levels of serum HPA, CRP and IL-6 were measured by ELISA, and the levels of blood glucose and blood lipids were also detected. Results: Compared with the control group, the levels of HPA, CRP, IL-6, FBG, HbA 1C , and TG of MIS group were all significantly elevated (all P<0.05), and HDL-C levels were considerably reduced (P<0.05). Correlation analysis showed that there was a noticeably positive correlation between serum HPA level and CRP, IL-6 levels (P<0.05). Conclusion:Higher HPA levels might play a certain role in the occurrence and development of MIS. There was a certain close correlation between serum HPA level and CRP and IL-6 levels, and which indicated that HPA was involved in the chronic low-grade inflammatory reaction process of MIS.

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Atherosclerosis: perspectives of anti-inflammatory therapy

Cited by 17 publications

References 0 publications

Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway

Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Serum Heparanase: A New Clinical Biomarker Involved in Senile Metabolic Inflammatory Syndrome

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