AtGRP3 Is Implicated in Root Size and Aluminum Response Pathways in Arabidopsis

Mangeon, Amanda; Pardal, Renan; Menezes-Salgueiro, Adriana Dias; Duarte, Guilherme Coutinho Kullmann; Seixas, Ricardo de; Cruz, Fernanda Pérez; Cardeal, Vanessa; Magioli, Cláudia; Ricachenevsky, Felipe Klein; Margis, Rogério; Sachetto-Martins, Gilberto

doi:10.1371/journal.pone.0150583

Cited by 34 publications

(34 citation statements)

References 51 publications

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“…Although wak1 exhibited increased Al sensitivity, transgenic lines overexpressing VuNAR1 by which WAK1 expression was greatly induced showed increased Al resistance. A recent study also suggests that the increased Al resistance in Atgrp3 mutant might be related to the increase of WAK1 free of AtGRP3 (Mangeon et al, ). Interestingly, such different expression levels of WAK1 were positively correlated with cell wall pectin content but not with hemicelluloses content (Figures and Figure S11).…”

Section: Discussionmentioning

confidence: 96%

A NAC‐type transcription factor confers aluminium resistance by regulating cell wall‐associated receptor kinase 1 and cell wall pectin

Lou

Fan

Jin

et al. 2019

Plant Cell & Environment

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Transcriptional regulation is important for plants to respond to toxic effects of aluminium (Al). However, our current knowledge to these events is confined to a few transcription factors. Here, we functionally characterized a rice bean (Vigna umbellata) NAC‐type transcription factor, VuNAR1, in terms of Al stress response. We demonstrated that rice bean VuNAR1 is a nuclear‐localized transcriptional activator, whose expression was specifically upregulated by Al in roots but not in shoot. VuNAR1 overexpressing Arabidopsis plants exhibit improved Al resistance via Al exclusion. However, VuNAR1‐mediated Al exclusion is independent of the function of known Al‐resistant genes. Comparative transcriptomic analysis revealed that VuNAR1 specifically regulates the expression of genes associated with protein phosphorylation and cell wall modification in Arabidopsis. Transient expression assay demonstrated the direct transcriptional activation of cell wall‐associated receptor kinase 1 (WAK1) by VuNAR1. Moreover, yeast one‐hybrid assays and MEME motif searches identified a new VuNAR1‐specific binding motif in the promoter of WAK1. Compared with wild‐type Arabidopsis plants, VuNAR1 overexpressing plants have higher WAK1 expression and less pectin content. Taken together, our results suggest that VuNAR1 regulates Al resistance by regulating cell wall pectin metabolism via directly binding to the promoter of WAK1 and induce its expression.

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Section: Discussionmentioning

confidence: 96%

A NAC‐type transcription factor confers aluminium resistance by regulating cell wall‐associated receptor kinase 1 and cell wall pectin

Lou

Fan

Jin

et al. 2019

Plant Cell & Environment

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“…Also, in Arabidopsis , increased expression of WAK1 , a gene encoding a cell wall kinase, significantly enhances Al tolerance [11]. Moreover, it has been reported that protein interacts with AtGRP3, a glycine-rich protein to control root size [12]. In fact, the outcome of AtWAK1/AtGRP3 interaction is usually cell elongation repression, which consequently results in shorter roots [12].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, it has been reported that protein interacts with AtGRP3, a glycine-rich protein to control root size [12]. In fact, the outcome of AtWAK1/AtGRP3 interaction is usually cell elongation repression, which consequently results in shorter roots [12]. It has been proposed that only free AtWAK1 (no AtGRP3 interaction) augments Al tolerance in Arabidopsis [11, 12].…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the outcome of AtWAK1/AtGRP3 interaction is usually cell elongation repression, which consequently results in shorter roots [12]. It has been proposed that only free AtWAK1 (no AtGRP3 interaction) augments Al tolerance in Arabidopsis [11, 12]. As consequence, Arabidopsis grp3-1 knockout mutants have longer roots and a higher tolerance to Al, mimicking the overexpression of AtWAK1 gene [11, 12].…”

Section: Introductionmentioning

confidence: 99%

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Full title: Label free quantitative proteomics ofQualea grandifloraMart. (Vochysiaceae) roots indicates an aluminium requirement for growth and development

Rcc

Melo

et al. 2018

Preprint

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Aluminium in acid soils is a hindrance to crop growth. Nonetheless, Brazilian Cerrado possesses many species such asQualea grandiflora, which are adapted to acid soils with large amounts of Al and accumulate this metal in its tissues and organs. Nonetheless, the mechanisms involved in these processes are poorly understood, mainly at molecular level. Thus, a root proteomic analysis was accomplished to identify Al-responsive proteins inQ. grandifloraplants. Concomitantly, a root growth analysis of plants grown with and without Al supplementation was conducted to determine the effects of Al on the whole plant. Subsequently, proteins from both treatments were identified and quantified by LC-MS/MS in a label-free fashion. From the 2,520 identified proteins, 410 were differentially abundant between the two treatments, which were associated with carbohydrate metabolism, redox activity, stress response and catabolism of organic compounds. Furthermore, Al was crucial for the growth and development ofQ. grandiflora. In fact, this species may have an Al-dependent metabolism. Moreover, it was possible to correlate plant growth to Al-upregulated proteins that were directly involved in cell wall synthesis, oxidative phosphorylation, genetic information processing, and amino acid metabolism. Additionally, this work provides an extensive dataset of Al-regulated protein inQ. grandiflora, which will be crucial to understanding the functions of Al in this species.

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“…Diverse plant species have different strategies for Al resistance; these have been described for various plants, including wheat (Delhaize et al, 1993; Garcia-Oliveira et al, 2013; Moustaka et al, 2016), barley (Furukawa et al, 2007; Ma et al, 2016), sorghum (Magalhaes et al, 2007; Caniato et al, 2014), rice (Ma et al, 2002; Yokosho et al, 2011; Xia et al, 2013; Arenhart et al, 2014), maize (Piñeros et al, 2002; Maron et al, 2013), Arabidopsis (Liu et al, 2009; Mangeon et al, 2016), snap bean (Miyasaka et al, 1991), buckwheat (Zhu et al, 2015), eucalyptus (Tahara et al, 2014), and hydrangea (Negishi et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Glutathione S-transferases and UDP-glycosyltransferases Are Involved in Response to Aluminum Stress in Flax

et al. 2016

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About 30% of the world's ice-free land area is occupied by acid soils. In soils with pH below 5, aluminum (Al) releases to the soil solution, and becomes highly toxic for plants. Therefore, breeding of varieties that are resistant to Al is needed. Flax (Linum usitatissimum L.) is grown worldwide for fiber and seed production. Al toxicity in acid soils is a serious problem for flax cultivation. However, very little is known about mechanisms of flax resistance to Al and the genetics of this resistance. In the present work, we sequenced 16 transcriptomes of flax cultivars resistant (Hermes and TMP1919) and sensitive (Lira and Orshanskiy) to Al, which were exposed to control conditions and aluminum treatment for 4, 12, and 24 h. In total, 44.9–63.3 million paired-end 100-nucleotide reads were generated for each sequencing library. Based on the obtained high-throughput sequencing data, genes with differential expression under aluminum exposure were revealed in flax. The majority of the top 50 up-regulated genes were involved in transmembrane transport and transporter activity in both the Al-resistant and Al-sensitive cultivars. However, genes encoding proteins with glutathione transferase and UDP-glycosyltransferase activity were in the top 50 up-regulated genes only in the flax cultivars resistant to aluminum. For qPCR analysis in extended sampling, two UDP-glycosyltransferases (UGTs), and three glutathione S-transferases (GSTs) were selected. The general trend of alterations in the expression of the examined genes was the up-regulation under Al stress, especially after 4 h of Al exposure. Moreover, in the flax cultivars resistant to aluminum, the increase in expression was more pronounced than that in the sensitive cultivars. We speculate that the defense against the Al toxicity via GST antioxidant activity is the probable mechanism of the response of flax plants to aluminum stress. We also suggest that UGTs could be involved in cell wall modification and protection from reactive oxygen species (ROS) in response to Al stress in L. usitatissimum. Thus, GSTs and UGTs, probably, play an important role in the response of flax to Al via detoxification of ROS and cell wall modification.

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AtGRP3 Is Implicated in Root Size and Aluminum Response Pathways in Arabidopsis

Cited by 34 publications

References 51 publications

A NAC‐type transcription factor confers aluminium resistance by regulating cell wall‐associated receptor kinase 1 and cell wall pectin

A NAC‐type transcription factor confers aluminium resistance by regulating cell wall‐associated receptor kinase 1 and cell wall pectin

Full title: Label free quantitative proteomics ofQualea grandifloraMart. (Vochysiaceae) roots indicates an aluminium requirement for growth and development

Glutathione S-transferases and UDP-glycosyltransferases Are Involved in Response to Aluminum Stress in Flax

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