AtGRDP1 Gene Encoding a Glycine-Rich Domain Protein Is Involved in Germination and Responds to ABA Signalling

Rodríguez-Hernández, Aida Araceli; Ortega-Amaro, María Azucena; Delgado‐Sánchez, Pablo; Salinas, Julio; Jiménez-Bremont, Juan Francisco

doi:10.1007/s11105-014-0714-4

Cited by 27 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the A. thaliana genome, in addition to AtGRDP2, we have identified three additional genes encoding proteins that also contain the DUF1399 domain, named AtGRDP1 (Rodríguez-Hernández et al, 2014 ), At1g56230, and At4g37682. The AtGRDP1 protein also contains the RNP-1 and glycine rich-region domains, while the At1g56230 and At4g37682 encoded proteins lack these domains (Figure S1C ).…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the canonical GRPs, Bocca et al ( 2005 ) reported the presence of glycine-rich domain proteins (GRDPs) transcripts in Eucalyptus , which encode proteins with a short glycine-rich domain. Recently, we reported the AtGRDP1 gene, encoding a short glycine-rich domain protein, which plays a regulatory role in ABA signaling and abiotic stress tolerance (Rodríguez-Hernández et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

et al. 2015

View full text Add to dashboard Cite

Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT, while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Three orthogroups have expanded in all resurrection plants compared with DS species: OG0212, OG1697, and OG2512. The Arabidopsis (Arabidopsis thaliana) orthologs in OG2512 include the Gly-rich domain protein AtGRDP1, which has a role in seed germination and ABA signaling (Rodríguez-Hernández et al, 2014). The AtGRDP1 null mutant has higher sensitivity to salt and osmotic stress, and overexpression increases stress tolerance (Rodríguez-Hernández et al, 2014).…”

Section: Patterns Of Gene Family Dynamics In Resurrection Plant Genomesmentioning

confidence: 99%

“…The Arabidopsis (Arabidopsis thaliana) orthologs in OG2512 include the Gly-rich domain protein AtGRDP1, which has a role in seed germination and ABA signaling (Rodríguez-Hernández et al, 2014). The AtGRDP1 null mutant has higher sensitivity to salt and osmotic stress, and overexpression increases stress tolerance (Rodríguez-Hernández et al, 2014). OG1697 contains orthologs related to the rice (Oryza sativa) domestication gene SH4, which is involved in seed shattering and development of the seed abscission layer (Li et al, 2006).…”

Section: Patterns Of Gene Family Dynamics In Resurrection Plant Genomesmentioning

confidence: 99%

Massive Tandem Proliferation of ELIPs Supports Convergent Evolution of Desiccation Tolerance across Land Plants

et al. 2019

View full text Add to dashboard Cite

Desiccation tolerance was a critical adaptation for the colonization of land by early nonvascular plants. Resurrection plants have maintained or rewired these ancestral protective mechanisms, and desiccation-tolerant species are dispersed across the land plant phylogeny. Although common physiological, biochemical, and molecular signatures are observed across resurrection plant lineages, features underlying the recurrent evolution of desiccation tolerance are unknown. Here we used a comparative approach to identify patterns of genome evolution and gene duplication associated with desiccation tolerance. We identified a single gene family with dramatic expansion in all sequenced resurrection plant genomes and no expansion in desiccationsensitive species. This gene family of early light-induced proteins (ELIPs) expanded in resurrection plants convergent through repeated tandem gene duplication. ELIPs are universally highly expressed during desiccation in all surveyed resurrection plants and may play a role in protecting against photooxidative damage of the photosynthetic apparatus during prolonged dehydration. Photosynthesis is particularly sensitive to dehydration, and the increased abundance of ELIPs may help facilitate the rapid recovery observed for most resurrection plants. Together, these observations support convergent evolution of desiccation tolerance in land plants through tandem gene duplication.

show abstract

“…These proteins domains are conserved in higher plant. GRPs domains play role during abiotic factors and ABA signaling, while the latter function is yet unknown completely (Yang et al, 2014;Rodríguez-Hernández et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)

Jamal¹,

Shahid²,

Aftab³

et al. 2019

PAK.J.BOT.

View full text Add to dashboard Cite

Cotton is major fiber crop among agricultural commodities grown worldwide. Prolonged osmotic stress and seasonal water shortage effect plant growth and yields. To explore the drought responsive molecular mechanism in cotton roots, we constructed cDNA library for the identification, functional annotation and expression analysis of potential drought responsive transcripts. mRNA extracted from cotton roots under drought stress was used to construct cDNA library. A total of 711 expressed sequence tags were generated from cDNA library. NCBI BLAST analysis of reported expressed sequence tags showed homology of 27% sequences with Gossypium species while 24% sequences showed no sequence similarity in non-redundant nucleotide database. Remaining 49% sequences revealed sequence similarity results with other plant species. Functional annotation was performed for sequenced EST's using Arabidopsis Gene ontology database (TAIR). We examined the expression pattern of 08 differentially expressed ESTs using RT-qPCR and validated that all these were upregulated in roots under osmotic stress, but to different folds. The candidate EST sequences identified in this study will be valuable resource to elucidate stress responsive molecular mechanism and genetic breeding of cotton in future.

show abstract

AtGRDP1 Gene Encoding a Glycine-Rich Domain Protein Is Involved in Germination and Responds to ABA Signalling

Cited by 27 publications

References 51 publications

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance

Massive Tandem Proliferation of ELIPs Supports Convergent Evolution of Desiccation Tolerance across Land Plants

Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)

Contact Info

Product

Resources

About