“…Atg8 can be conjugated to lipid phosphatidylethanolamine (PE) through a ubiquitin-like conjugative reaction to form an Atg8–PE complex anchored to the membrane, and the lipidation of Atg8 is necessary for autophagy (Ichimura et al, 2000). Atg8’s autophagy-independent functions, including those in vesicular transport, resistance to oxidative stress, vacuolar fusion, and the formation of lipid bodies, have happened not to require the lipidation of Atg8 (Legesse-Miller et al, 2000; Maeda et al, 2017; Mikawa et al, 2010; Tamura et al, 2010). Since the Atg8’s suppressing function of vacuolar invagination after heat stress does not need its lipidation, it was proposed to be one of autophagy-independent functions (Ishii et al, 2019).…”