Aterogênese em artéria ilíaca comum de suínos submetidos à homocisteinemia induzida pela ingestão de metionina

França, Luís Henrique Gil; Pereira, Adamastor Humberto; Perini, Silvana; Aveline, Celso Curcio; Argenta, Rodrigo; Mollerke, Roseli de Oliveira; Soares, Marcos Eugênio; Nóbrega, Fernanda Silveira; Ferreira, Márcio Poletto

doi:10.1590/s1677-54492006000100003

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…To avoid such problems and promote early atherosclerosis in pigs, we used a 30-day methionine–rich diet to promote arterial lesions. These lesions were similar to those previously described by Rolland et al,13 and we have previously shown that a 30-day methionine–rich diet consistently promotes homocysteine arterial lesions in pig iliac arteries21 and rabbit aortas 20…”

Section: Methodssupporting

confidence: 91%

Self-Expandable Nitinol Stent Placement in Homocysteinemic Porcine Aorta

França

Pereira

Perini

2008

Clinics

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França LHG, Pereira AH, Perini SC. Self-expandable nitinol stent placement in homocysteinemic porcine aorta. Clinics. 2008;63:229-36. PURPOSE:To compare aortic intimal thickening of normal and hyperhomocysteinemic pigs (induced with a methionine-rich diet) following placement of a self-expanding nitinol stent. METHODS: Eighteen Macau pigs were used. They were older than eight weeks in age and had an average weight of 30 kg. Pigs were randomly divided into two groups. The first, Group C (control), was fed a regular diet, and the second group, Group M, was fed a methionine-rich diet for 30 days to induce hyperhomocysteinemia. The self-expandable nitinol stents were 25mm in length and 8 mm in diameter after expansion. Blood samples were collected to measure total cholesterol, triglycerides, HDL and homocysteine concentrations. All animals were subjected to angiography. Thirty days after the procedure, the animals were sacrificed, and the abdominal aorta was removed for histological and digital morphometry analysis. RESULTS: Under microscopic evaluation, the intima was significantly thicker in Group C than in Group M. When groups were compared by digital morphometric analysis, intimal thickening of the vessel wall was higher in Group C than in Group M. There was no significant change in total cholesterol, triglycerides or HDL concentrations in either group. In group C the levels of plasma homocysteine ranged from 14,40 to 16,73µmol/l; in Group M, plasma homocysteine levels ranged from 17.47 to 59.80 µmol/l after 30 days of a methionine-rich diet. CONCLUSION: Compared to normal pigs, less intimal hyperplasia was observed in the abdominal aortas of hyperhomocysteinemic pigs thirty days after the insertion of a self-expandable nitinol stent.

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Section: Methodssupporting

confidence: 91%

Self-Expandable Nitinol Stent Placement in Homocysteinemic Porcine Aorta

França

Pereira

Perini

2008

Clinics

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“…Pesquisas experimentais nacionais e recentes reforçaram o papel da hiper-homocisteinemia induzida pela sobrecarga de metionina na formação de placa ateroscleróti-ca de aorta em suínos 42 e artéria ilíaca em coelhos 43 . Após período de 30 ou 60 dias de dieta rica em metionina, houve aumento significante nas concentrações de homocisteína e na formação de placas ateroscleróticas por células espumosas, mas não foram observadas células musculares lisas, cristais de colesterol ou células inflamatórias.…”

Section: Mecanismos Patogênicos Da Hiper-homocisteinemiaunclassified

Tratamento dietético da hiper-homocisteinemia na doença arterial periférica

Venâncio

Burini²,

Yoshida

2010

J. vasc. bras.

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IntroduçãoA doença arterial periférica (DAP) é definida como uma doença arterial das extremidades que reduz o fluxo sanguíneo durante o exercício ou, em estágios avançados, mesmo em repouso 1 . Estudos epidemiológicos mostram que a prevalência de DAP varia de 1,6 a 12% 2,3 .As doenças do aparelho circulatório foram as principais causas de morte entre os brasileiros em 2007 (28,2%) 4 e na população mundial (18 milhões) 5 ; dentre as várias etiologias, a aterosclerose foi a causa mais comum. Entre os pacientes com doença aterosclerótica sintomática, 15,9% têm doença polivascular sintomáti-ca (DAP, doença cardiovascular e cerebrovascular) 6 .Além disso, na fase de claudicação intermitente, cerca de 65% dos indivíduos apresentam comprometimento simultâneo e importante dos setores coronariano e cerebrovascular, e 30% dos pacientes com doença vascular coronariana ou cerebral apresentam DAP 7 . Desse modo, a DAP está associada a um aumento da morbimortalidade por doenças cardiovasculares (aproximadamente 30%) e cerebrovasculares 8 , sendo um importante preditor das mesmas. ARTIGO DE REVISÃO ResumoA homocisteína está envolvida na gênese da aterosclerose e, assim, é considerada um importante e prevalente fator de risco na doença arterial periférica. O estado nutricional vitamínico deficiente, em especial do folato, é a principal causa de hiper-homocisteinemia nesses casos. Embora ainda não haja consenso sobre a dose exata e a forma de utilização do folato em suplementos e sobre adequação alimentar ou fortificação de cereais para o tratamento da hiper-homocisteinemia, diversos estudos realizados em pacientes com doença vascular periférica mostraram que o folato, isoladamente, pode reduzir as concentrações de homocisteína, bem como a concentração de alguns marcadores biológicos do processo de aterosclerose. No entanto, estudos recentes não comprovaram esse benefício sobre o processo inflamatório associado à hiper-homocisteinemia. Desta forma, embora a utilização isolada do folato seja uma terapêutica custo-efetiva no controle da hiper-homocisteinemia, seu impacto na evolução das doenças arteriais ainda persiste inconclusivo. Esta revisão abordará os efeitos obtidos com as diversas formas de utilização do folato no tratamento da hiper-homocisteinemia.Palavras-chave: Homocisteína, aterosclerose, vitamina, ácido fó-lico. AbstractHomocysteine plays a role in the genesis of atherosclerosis and, thus, it is considered an important and prevalent risk factor for peripheral arterial disease. Impaired vitamin nutritional status, especially regarding folate, may be mainly attributed to hyperhomocysteinemia. Although there is still no consensus as to the exact dose and method of use of folate in supplements, dietary adjustment or cereal fortification for the treatment of hyperhomocysteinemia, several studies conducted in patients with peripheral vascular disease have shown that isolated folate may reduce homocysteine levels, as well as the levels of some biological markers in the atherosclerotic process. However, recent studies ha...

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“…The depressing effect of Met on cholesterol in the mammalian body may be related to Met's ability to generates the lipid peroxidation of cell membrane components (cholesterol, gangliosides, glycosphingolipids glycerophospholipids and unsaturated), as high rates of lipid peroxidation (TBARS) were observed in the cerebral cortex of rats subjected to Met infusion (Stefanello et al, 2005), but this response needs to be further investigated. By the other side, França et al (2006) evaluating two diets for starting pigs (4.6 and 34.6 g/kg of SID Met + Cys) reported that serum cholesterol was not affected by treatments, but the authors observed higher homocysteine levels when adding synthetic Met in the diet.…”

Section: Discussionmentioning

confidence: 99%

Maintaining the same ratio between standardized ileal digestible methionine and cysteine do not affect its requirements for starting pigs

et al. 2021

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This study aimed to determine the requirements of standardized ileal digestible (SID) methionine (Met) + cysteine (Cys) of 15–30 kg barrows, maintaining or increasing the 1:1 ratio between SID Met and SID Cys. Seventy crossbred barrows averaging 15.2 ± 0.54 kg of live weight were allotted in a randomized block design 2 × 3 + 1 factorial scheme, with five replicates and two animals per pen. Treatments consisted of maintaining or increasing the 1:1 ratio between SID Met and SID Cys, three levels of SID Met + Cys (5.8, 6.4 and 7.0 g/kg) and a basal diet containing the lowest SID Met + Cys level (5.2 g/kg), formulated to provide a 1:1 ratio of SID Met (2.6 g/kg) and SID Cys (2.6 g/kg). Performance, blood parameters, longissimus dorsi muscle depth, backfat thickness and S–S linkages in the bristles were evaluated. The best average daily gain was estimated at 6.61 g/kg of SID Met + Cys, without maintaining the 1:1 ratio between sulphur amino acids (SAA). The daily intake of SID Met + Cys increased due to dietary SID Met + Cys levels, maintaining or increasing the same ratio between SAA. Plasma glucose increased and total cholesterol decreased according to SID Met + Cys levels, without maintaining the same ratio between the SAA. The requirement of SID Met + Cys for 15–30 kg barrows was 6.61 g/kg (7.88 g/day) for an optimum average daily gain, with no need to maintain the same ratio between the SAA.

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Aterogênese em artéria ilíaca comum de suínos submetidos à homocisteinemia induzida pela ingestão de metionina

Cited by 8 publications

References 14 publications

Self-Expandable Nitinol Stent Placement in Homocysteinemic Porcine Aorta

Self-Expandable Nitinol Stent Placement in Homocysteinemic Porcine Aorta

Tratamento dietético da hiper-homocisteinemia na doença arterial periférica

Maintaining the same ratio between standardized ileal digestible methionine and cysteine do not affect its requirements for starting pigs

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