Atelocollagen-Based Gene Transfer in Cells Allows High-Throughput Screening of Gene Functions

Honma, Kimi; Ochiya, Takahiro; Nagahara, Shunji; Sano, Akihiko; Yamamoto, Hiroyasu; Hirai, Kotaro; Aso, Yu; Terada, Masaaki

doi:10.1006/bbrc.2001.6133

Cited by 79 publications

(55 citation statements)

References 24 publications

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“…5 Although the precise mechanisms by which ATCOL achieves these effects have not been elucidated to date, ATCOL complexed with DNA molecules was demonstrated to be efficiently transduced into mammalian cells. 19 Thus, similarly siRNA/ATCOL complexes may be transduced into cells probably by the same mechanisms as observed for DNA molecules. As a simple administration of myostatin-siRNA/ATCOL complex has a muscle growth effect, this novel method for fighting against muscle atrophy would be of considerable value for clinical applications.…”

mentioning

confidence: 88%

Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass

et al. 2008

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mentioning

confidence: 88%

Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass

et al. 2008

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“…As an alternative to manipulating cell adhesion, the deposition of the vector can be controlled. The spatially controlled deposition of gene therapy vectors can be achieved by several methods, such as spotting, printing, microfluidics, or pinning (i.e., the aqueous vector solution wets the hydrophilic but not hydrophobic region) [151][152][153][154][155][156]. The deposition procedure must retain vector activity, while the vector-material interactions must maintain the vector locally.…”

Section: Spatially Patterned Gene Deliverymentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

240

208

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Regenerative medicine aims to create functional tissue replacements, typically through creating a controlled environment that promotes and directs the differentiation of stem or progenitor cells, either endogenous or transplanted. Scaffolds serve a central role in many strategies by providing the means to control the local environment. Gene delivery from the scaffold represents a versatile approach to manipulating the local environment for directing cell function. Research at the interface of biomaterials, gene therapy, and drug delivery has identified several design parameters for the vector and the biomaterial scaffold that must be satisfied. Progress has been made towards achieving gene delivery within a tissue engineering scaffold, though the design principles for the materials and vectors that produce efficient delivery require further development. Nevertheless, these advances in obtaining transgene expression with the scaffold have created opportunities to develop greater control of either delivery or expression and to identify the best practices for promoting tissue formation. Strategies to achieve controlled localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.

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“…Transfected cell arrays have been formed using a substrate-mediated approach in which plasmids or adenoviruses were mixed with collagen and spotted onto glass slides or into wells [46][47][48]. Plated cells were transfected and could be analyzed for cellular responses using a variety of imaging or biochemical techniques.…”

Section: Patterned Transfectionmentioning

confidence: 99%

“…Also, DNA complexes have been mixed with fibronectin and spotted onto glass slides for arrayed transfection of human mesenchymal stem cells [51]. While transfected cell arrays hold great potential [46][47][48][49][50][51], further development of a substratemediated delivery system that efficiently transfects a wide variety of primary cells and cell lines, while allowing for spatially-controlled delivery within the different domains is required [46,52,53]. Using SAMs on gold to form transfected cell arrays can allow precise control over surface chemistries, interactions between the substrate and DNA complexes, transfection efficiencies, and pattern sizes to create a well-characterized and efficient array delivery system.…”

Section: Patterned Transfectionmentioning

confidence: 99%

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, selfassembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.

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Atelocollagen-Based Gene Transfer in Cells Allows High-Throughput Screening of Gene Functions

Cited by 79 publications

References 24 publications

Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass

Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass

Matrices and scaffolds for DNA delivery in tissue engineering

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

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