AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem

Lin, Zhiqiang; Yin, Kangquan; Zhu, Danling; Chen, Zhangliang; Gu, Hongya; Liu, Qu

doi:10.1038/cr.2007.71

Cited by 64 publications

(65 citation statements)

References 51 publications

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“…causes both immunity and pleiotropic development defects, which agrees with the crucial roles of miRNAs in regulating multiple biological processes (31,32). However, it is possible that the regulation of genes other than small RNAs by CDC5 also contributes to the observed phenotypes in cdc5-1.…”

Section: Discussionmentioning

confidence: 63%

“…In cdc5-1, a T-DNA insertion disrupts the expression of CDC5, resulting in multiple developmental defects such as smaller plant size, altered leaf shape, late flowering, and sterility (31,32). We reasoned that cdc5-1 might impair miRNA accumulation, as the alteration in miRNA levels often causes pleiotropic developmental defects (33,34).…”

Section: Cdc5 Ismentioning

confidence: 99%

“…In human and yeast, CDC5 has been shown to act as a component of spliceosome to participate in mRNA splicing (29,30). In Arabidopsis, CDC5 binds DNA and is required for development and immunity to bacteria infection (31,32). However, how CDC5 functions in Arabidopsis is unclear.…”

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confidence: 99%

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CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

Zhang

Xie

Ren

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

134

160

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CDC5 is a MYB-related protein that exists in plants, animals, and fungi. In Arabidopsis, CDC5 regulates both growth and immunity through unknown mechanisms. Here, we show that CDC5 from Arabidopsis positively regulates the accumulation of microRNAs (miRNAs), which control many biological processes including development and adaptations to environments in plants. CDC5 interacts with both the promoters of genes encoding miRNAs (MIR) and the DNA-dependent RNA polymerase II. As a consequence, lack of CDC5 reduces the occupancy of polymerase II at MIR promoters, as well as MIR promoter activities. In addition, CDC5 is associated with the DICER-LIKE1 complex, which generates miRNAs from their primary transcripts and is required for efficient miRNA production. These results suggest that CDC5 may have dual roles in miRNA biogenesis: functioning as a positive transcription factor of MIR and/or acting as a component of the DICER-LIKE1 complex to enhance primary miRNA processing.M icroRNAs (miRNAs) and small interfering RNAs (siRNAs) are ∼22-nucleotide (nt) noncoding RNAs that regulate various biological processes including development, metabolism, and immunity in plants and animals (1-3). miRNAs are generated from primary miRNA transcripts (pri-miRNAs) containing stem-loop structure, whereas siRNAs are derived from long, perfect, doublestranded RNAs (dsRNAs) (1-3). They are associated with members of the Argonaute protein family to repress gene expression at posttranscriptional and/or transcriptional levels (1-3). In addition to miRNAs, plants encode two major classes of siRNAs: siRNAs derived from repeated DNAs (ra-siRNAs) and transacting siRNAs (ta-siRNAs) (4-6).Studies in Arabidopsis have established the framework of miRNA biogenesis in plants (1-3). In Arabidopsis, pri-miRNAs are primarily transcribed by DNA-dependent RNA polymerase II (Pol II), with assistance from the mediator complex and the transcription factor Negative on TATA less2 (NOT2) (7,8). After transcription, pri-miRNAs are processed by an RNase III enzyme called DICER-LIKE1 (DCL1) to miRNA precursors and then to mature miRNAs (9, 10). The efficient processing of pri-miRNA requires SERRATE (SE; a zinc finger protein), TOUGH (an RNA-binding protein), and a dephosphorylated HYPONASTIC LEAVES1 (HYL1; a double-stranded RNA binding protein) that form a complex with DCL1 (11-18). SE and HYL1 also promote the processing accuracy of pri-miRNAs (19). Four other proteins, DAWDLE (DDL; an RNA binding protein), Cap-binding protein 20, Cap-binding protein 80, and NOT2, which are associated with the DCL1 complex (8,(20)(21)(22), also function in miRNA biogenesis. Recent studies also reveal that the correct localization of DCL1 requires NOT2 and MODIFIER OF SNC1, 2 (an RNA binding protein) (8,23). In addition, the accumulation of a subset of miRNAs requires a proline-rich protein named SICKLE (24).The cell division cycle 5 (CDC5) protein is a conserved protein in animals, plants, and fungi (25). It was first isolated from Schizosaccharomyces pombe as a cell cycle regulat...

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Section: Discussionmentioning

confidence: 63%

Section: Cdc5 Ismentioning

confidence: 99%

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CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

Zhang

Xie

Ren

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

134

160

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“…Detailed characterization of these genes should determine their roles in cell cycle progression. AtCDC5, an Arabidopsis homolog of yeast cdc5, plays roles in the cell cycle that are similar to those of yeast cdc5 [15,30]. The AtCDC5 MYB domain has two repeats that are only distantly related to those of the R2R3-type MYB domain [5,15].…”

Section: Discussionmentioning

confidence: 99%

An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis

et al. 2009

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“…Also the MYB-related CDC5-like proteins, characterized in different species (yeast, animal and AtCDC5 in Arabidopsis), harboring a MYB domain with two repeats only distantly related to those of the R2R3-type MYB proteins, control the G2/M transition of the cell cycle [6].…”

mentioning

confidence: 99%

A new role for plant R2R3-MYB transcription factors in cell cycle regulation

Cominelli

Tonelli

2009

Cell Res

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AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem

Cited by 64 publications

References 51 publications

CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis

A new role for plant R2R3-MYB transcription factors in cell cycle regulation

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