Atalantums H-K from the peels of <i>Atalantia monophylla</i> and their cytotoxicity

Sombatsri, Aonnicha; Thummanant, Yutthapong; Sribuhom, Thurdpong; Wongphakham, Paweena; Senawong, Thanaset; Yenjai, Chavi

doi:10.1080/14786419.2019.1576042

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…CE and L1 cytotoxic activity were evaluated against Vero and MCF-7 cell lines. Vero cells are used worldwide as a normal cell line control to assess in vitro cytotoxic effects [26,27], due to its susceptibility to various types of microbes, toxins, and chemical compounds. This cell line is often used in natural product screening assays [28,29,30] and in cancer studies as a normal cell line control group [31].…”

Section: Resultsmentioning

confidence: 99%

Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants

et al. 2019

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Macroalgae represent an important source of bioactive compounds with a wide range of biotechnological applications. Overall, the discovery of effective cytotoxic compounds with pharmaceutical potential is a significant challenge, mostly because they are scarce in nature or their total synthesis is not efficient, while the bioprospecting models currently used do not predict clinical responses. Given this context, we used three-dimensional (3D) cultures of human breast cancer explants to evaluate the antitumoral effect of laurinterol, the major compound of an ethanolic extract of Laurencia johnstonii. To this end, we evaluated the metabolic and histopathological effects of the crude extract of L. johnstonii and laurinterol on Vero and MCF-7 cells, in addition to breast cancer explants. We observed a dose-dependent inhibition of the metabolic activity, as well as morphologic and nuclear changes characteristic of apoptosis. On the other hand, a reduced metabolic viability and marked necrosis areas were observed in breast cancer explants incubated with the crude extract, while explants treated with laurinterol exhibited a heterogeneous response which was associated with the individual response of each human tumor sample. This study supports the cytotoxic and antitumoral effects of laurinterol in in vitro cell cultures and in ex vivo organotypic cultures of human breast cancer explants.

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Section: Resultsmentioning

confidence: 99%

Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants

et al. 2019

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“…The MCE-7 (ATCC® HTB-22T M ) and Vero cells (ATCC® CCL-81™) were obtained from the Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Terengganu, Malaysia, while the 4T1 cells were from ATCC (ATCC® CRL-2539™). The Vero cells are the most common mammalian continuous cell line used in research and suitable for the screening assays of natural products, in vitro studies [ 25 , 26 , 27 , 28 , 29 , 30 ], and are usually used in cancer studies as normal cell lines [ 27 ]. Vero cells have been tested extensively in the production of the vaccine and found to be free from the symptomatic agents which could have reduced the sensitivity of the tests.…”

Section: Methodsmentioning

confidence: 99%

Microalgal metabolites as anti-cancer/anti-oxidant agents reduce cytotoxicity of elevated silver nanoparticle levels against non-cancerous vero cells

Hussein

Maulidiani

Abdullah

2020

Heliyon

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Heavy metal pollution has become a major concern globally as it contaminates eco-system, water networks and as finely suspended particles in air. In this study, the effects of elevated silver nanoparticle (AgNPs) levels as a model system of heavy metals, in the presence of microalgal crude extracts (MCEs) at different ratios, were evaluated against the non-cancerous Vero cells, and the cancerous MCF-7 and 4T1 cells. The MCEs were developed from water (W) and ethanol (ETH) as green solvents. The AgNPs-MCEs-W at the 4:1 and 5:1 ratios (v/v) after 48 and 72 h treatment, respectively, showed the IC 50 values of 83.17–95.49 and 70.79–91.20 μg/ml on Vero cells, 13.18–28.18 and 12.58–25.7 μg/ml on MCF-7; and 16.21–33.88 and 14.79–26.91 μg/ml on 4T1 cells. In comparison, the AgNPs-MCEs-ETH formulation achieved the IC 50 values of 56.23–89.12 and 63.09–91.2 μg/ml on Vero cells, 10.47–19.95 and 13.48–26.61 μg/ml on MCF-7; 14.12–50.11 and 15.13–58.88 μg/ml on 4T1 cells, respectively. After 48 and 72 h treatment, the AgNPs-MCE-CHL at the 4:1 and 5:1 ratios exhibited the IC 50 of 51.28–75.85 and 48.97–69.18 μg/ml on Vero cells, and higher cytotoxicity at 10.47–16.98 and 6.19–14.45 μg/ml against MCF-7 cells, and 15.84–31.62 and 12.58–24.54 μg/ml on 4T1 cells, respectively. The AgNPs-MCEs-W and ETH resulted in low apoptotic events in the Vero cells after 24 h, but very high early and late apoptotic events in the cancerous cells. The Liquid Chromatography-Mass Spectrometry-Electrospray Ionization (LC-MS-ESI) metabolite profiling of the MCEs exhibited 64 metabolites in negative ion and 56 metabolites in positive ion mode, belonging to different classes. The microalgal metabolites, principally the anti-oxidative components, could have reduced the toxicity of the AgNPs against Vero cells, whilst retaining the cytotoxicity against the cancerous cells.

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“…Vero cells are widely used in research as established mammalian cell lines and have been used as a control normal cell line in the evaluation of cytotoxic effects ( Fernández Freire et al., 2009 ; Sombatsri et al., 2019 ). This cell line is usually used in cancer studies ( Siddiqui et al., 2019 ) and natural product screening tests ( Mashjoor et al., 2015 ; Sit et al., 2018 ; Kumarihamy et al., 2019 ), because of their sensitivity to different types of microbes, toxins, and chemical complexes.…”

Section: Resultsmentioning

confidence: 99%