AtA6PR1 and AtA6PR2 encode putative aldose 6-phosphate reductases that are cytosolically localized and respond differentially to cold and salt stress in Arabidopsis thaliana

Rojas, Bárbara; Wurman, Joel; Zamudio, María Sofía; Donoso, Amanda; Cabedo, Pamela; Díaz, Francisca; Stange, Claudia; Handford, Michael

doi:10.1007/s13562-018-0459-5

Cited by 7 publications

(9 citation statements)

References 39 publications

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“…The study of M. truncatula AKRs can provide a foundation for the study of legume forage AKR genes. The MtAKRs have an average of 336 amino acids and an average protein mass of 37 kDa in MtAKRs, which is consistent with the previous published that most AKRs contain approximately 320 amino acids, with proteins 33-37 kDa in size [8,39,40]. The differences in transcriptional induction can be attributed to the quality and numbers of the cis-elements present in the promoters [41].…”

Section: Characterization and Analysis Of The Mtakr Gene Familysupporting

confidence: 90%

“…These suggest the similar function of MtAKR5, MtAKR8, and MtAKR17. The expression pattern of MtAKR5 was detected in this study ( Figure 6) had a similar response to that of AtA6PR1 under NaCl treatment [40].…”

Section: The Phylogenetic Analysis Of Close Relationship Between a Tsupporting

confidence: 73%

“…Results showed that the highest expression levels of MtAKR22 and MtAKR29 were present at 8 or 24 h after salt, drought, and ABA stresses, both in roots and leaves ( Figure 6). AT1G59950 (AtA6PR1) and AT1G59960 (AtA6PR2) in subgroup I-1 were previously proposed to be related to the aldose 6-phosphate reductase pathway, and they both exhibited response to cold and salt stresses [40]. The sequences of MtAKR5, MtAKR8, and MtAKR17 are in the same cluster with these genes ( Figure 5).…”

Section: The Phylogenetic Analysis Of Close Relationship Between a Tmentioning

confidence: 82%

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Analysis of Aldo–Keto Reductase Gene Family and Their Responses to Salt, Drought, and Abscisic Acid Stresses in Medicago truncatula

Sun

Zhang

et al. 2020

IJMS

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Salt and drought stresses are two primary abiotic stresses that inhibit growth and reduce the activity of photosynthetic apparatus in plants. Abscisic acid (ABA) plays a key role in abiotic stress regulation in plants. Some aldo–keto reductases (AKRs) can enhance various abiotic stresses resistance by scavenging cytotoxic aldehydes in some plants. However, there are few comprehensive reports of plant AKR genes and their expression patterns in response to abiotic stresses. In this study, we identified 30 putative AKR genes from Medicago truncatula. The gene characteristics, coding protein motifs, and expression patterns of these MtAKRs were analyzed to explore and identify candidate genes in regulation of salt, drought, and ABA stresses. The phylogenetic analysis result indicated that the 52 AKRs in Medicago truncatula and Arabidopsis thaliana can be divided into three groups and six subgroups. Fifteen AKR genes in M. truncatula were randomly selected from each group or subgroup, to investigate their response to salt (200 mM of NaCl), drought (50 g·L−1 of PEG 6000), and ABA (100 µM) stresses in both leaves and roots. The results suggest that MtAKR1, MtAKR5, MtAKR11, MtAKR14, MtAKR20, and MtAKR29 may play important roles in response to these stresses.

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Section: Characterization and Analysis Of The Mtakr Gene Familysupporting

confidence: 90%

Section: The Phylogenetic Analysis Of Close Relationship Between a Tsupporting

confidence: 73%

Section: The Phylogenetic Analysis Of Close Relationship Between a Tmentioning

confidence: 82%

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Analysis of Aldo–Keto Reductase Gene Family and Their Responses to Salt, Drought, and Abscisic Acid Stresses in Medicago truncatula

Sun

Zhang

et al. 2020

IJMS

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“…Direct phosphorylation of recombinant aldose reductase by protein kinase C was demonstrated in vitro , although no experiments were performed to evaluate neither the effect of this PTM on the kinetic properties of the enzyme nor the identity of the phosphorylated residue explored (Varma et al, 2003). The PhosPhat database (Durek et al, 2009; Heazlewood et al, 2008; Zulawski et al, 2013) indicates that the Arabidopsis At2G21260 protein, an ortholog of peach Ald6PRase with demonstrated Ald6PRase activity (Rojas et al, 2019), is phosphorylated at Thr 256 . Our mass spectrometry analysis failed to identify whether or not the corresponding residue in peach Ald6PRase (Thr 257 ) was phosphorylated.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of aldose-6-phosphate reductase from Prunus persica leaves

Hartman

Rojas

Ferrero

et al. 2022

Preprint

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Sugar-alcohols are major photosynthates in plants from the Rosaceae family. Expression of the gene encoding aldose-6-phosphate reductase (Ald6PRase), the critical enzyme for glucitol synthesis in rosaceous species, is regulated by physiological and environmental cues. Additionally, Ald6PRase is inhibited by small molecules (hexose-phosphates and inorganic orthophosphate) and oxidizing compounds. This work demonstrates that Ald6PRase from peach leaves is phosphorylated in planta at the N-terminus. We also show in vitro phosphorylation of recombinant Ald6PRase by a partially purified kinase extract from peach leaves containing Ca2+-dependent protein kinases (CDPKs). Moreover, phosphorylation of recombinant Ald6PRase was inhibited by hexose-phosphates, phosphoenolpyruvate and pyrophosphate. We further show that phosphorylation of recombinant Ald6PRase was maximal using recombinant CDPKs. Overall, our results suggest that phosphorylation could fine-tune the activity of Ald6PRase.

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“…S6PDH, namely aldose 6-phosphate reductase, is a vital enzyme in sorbitol biosynthesis [29]. Its expression is closely related to photosynthesis, source-sink intensity and distribution of photosynthetic products, and has an obvious relationship with the plant's resistance to abiotic and biotic stresses [30]. The consistent upregulation of RFS and S6PDH genes in noni fruit are likely to increase the defense resistance and oxidation resistance of the fruit after harvesting, which consumes energy.…”

Section: Disordered Energy Metabolism Enrichment Of Degs In Galactose Metabolism and Downregulation Of D-tagatosementioning

confidence: 99%

Noni fruit’s water spot appearance on the second day of harvest: a trade-off between resistance and energy

Wang

2021

Chem. Biol. Technol. Agric.

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Background Noni (Morinda citrifolia Linn.) is a tropical tree that bears climacteric fruit. Previous observations and research have shown that the second day (2 d) after harvest is the most important demarcation point when the fruit has the same appearance as the freshly picked fruit (0 d); however, they are beginning to become water spot appearance. We performed a conjoint analysis of metabolome and transcriptome data for noni fruit of 0 d and 2 d to reveal what happened to the fruit at the molecular level. Genes and metabolites were annotated to KEGG pathways and the co-annotated KEGG pathways were used as a statistical analysis. Results We found 25 pathways that were significantly altered at both metabolic and transcriptional levels, including a total of 285 differentially expressed genes (DEGs) and 11 differential metabolites through an integrative analysis of transcriptomics and metabolomics. The energy metabolism and pathways originating from phenylalanine were disturbed the most. The upregulated resistance metabolites and genes implied the increase of resistance and energy consumption in the postharvest noni fruit. Most genes involved in glycolysis were downregulated, further limiting the available energy. This lack of energy led noni fruit to water spot appearance, a prelude to softening. The metabolites and genes related to the resistance and energy interacted and restricted each other to keep noni fruit seemingly hard within two days after harvest, but actually the softening was already unstoppable. Conclusions This study provides a new insight into the relationship between the metabolites and genes of noni fruit, as well as a foundation for further clarification of the post-ripening mechanism in noni fruit.

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AtA6PR1 and AtA6PR2 encode putative aldose 6-phosphate reductases that are cytosolically localized and respond differentially to cold and salt stress in Arabidopsis thaliana

Cited by 7 publications

References 39 publications

Analysis of Aldo–Keto Reductase Gene Family and Their Responses to Salt, Drought, and Abscisic Acid Stresses in Medicago truncatula

Analysis of Aldo–Keto Reductase Gene Family and Their Responses to Salt, Drought, and Abscisic Acid Stresses in Medicago truncatula

Phosphorylation of aldose-6-phosphate reductase from Prunus persica leaves

Noni fruit’s water spot appearance on the second day of harvest: a trade-off between resistance and energy

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