At the edge of translation – materials to program cells for directed differentiation

Arany, Praveen R.; Mooney, David J.

doi:10.1111/j.1601-0825.2010.01735.x

Cited by 16 publications

(16 citation statements)

References 98 publications

(111 reference statements)

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“…Tailoring the degradation properties of constructs based on the kinetics of cell proliferation, inflammation, and angiogenesis could provide a means for efficient integration. Overall, the development of new biomaterials platforms that can collectively modulate host cell recruitment and material-resident cell function, as well as engraftment and release of implanted cell types (197), is a major focus and should advance liver tissue engineering approaches.…”

Section: Translating Existing Technology Into Pre-clinical and Therapmentioning

confidence: 99%

Cell and tissue engineering for liver disease

et al. 2014

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Despite the tremendous hurdles presented by the complexity of the liver’s structure and function, advances in liver physiology, stem cell biology and reprogramming, and the engineering of tissues and devices are accelerating the development of cell-based therapies for treating liver disease and liver failure. This State of the Art Review discusses both the near and long-term prospects for such cell-based therapies and the unique challenges for clinical translation.

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Section: Translating Existing Technology Into Pre-clinical and Therapmentioning

confidence: 99%

Cell and tissue engineering for liver disease

et al. 2014

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“…While investigating the mechanisms of biophysical interventions, care should be taken to focus on identifying the critical pivotal factor(s) (causal events) that will aid in gaining mechanistic insights. 4 A useful yardstick often employed in the distinction of causal versus effector mechanisms is the temporal (timedependent) separation following an intervention. However, this is largely restrictive due to current technical limitations of assessing rapid biological events, both at temporal and spatial length scales.…”

Section: Introductionmentioning

confidence: 99%

Biophysical Approaches for Oral Wound Healing: Emphasis on Photobiomodulation

Khan

Arany

2015

Advances in Wound Care

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“…Here we describe a new approach for biomaterials-based morphogen presentation that was inspired by processes of embryonic development, where a uniform mass of undifferentiated cells develops into morphologically distinct, multi-lineage tissues in an organism under the direction of tightly regulated morphogen signaling (Arany and Mooney, 2011). In particular, developmental processes often depend on the activity of not a single, positive, signal, but instead often utilize two cues, one positive and one inhibitory, that are released from distinct spatial locations.…”

mentioning

confidence: 99%

Multi-lineage MSC Differentiation via Engineered Morphogen Fields

Arany

Huang²,

Gadish³

et al. 2014

J Dent Res

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Tissue loss due to oral diseases requires the healing and regeneration of tissues of multiple lineages. While stem cells are native to oral tissues, a current major limitation to regeneration is the ability to direct their lineage-specific differentiation. This work utilizes polymeric scaffold systems with spatiotemporally controlled morphogen cues to develop precise morphogen fields to direct mesenchymal stem cell differentiation. First, a simple three-layer scaffold design was developed that presented two spatially segregated, lineage-specific cues (Dentinogenic TGF-β1 and Osteogenic BMP4). However, this system resulted in diffuse morphogen fields, as assessed by the in vitro imaging of cell-signaling pathways triggered by the morphogens. Mathematical modeling was then exploited, in combination with incorporation of specific inhibitors (neutralizing antibodies or a small molecule kinase inhibitor) into each morphogen in an opposing spatial pattern as the respective morphogen, to design a five-layer scaffold that was predicted to yield distinct, spatially segregated zones of morphogen signaling. To validate this system, undifferentiated MSCs were uniformly seeded in these scaffold systems, and distinct mineralized tissue differentiation were noted within these morphogen zones. Finally, to demonstrate temporal control over morphogen signaling, latent TGF-β1 was incorporated into one region of a concentric scaffold design, and laser treatment was used to activate the morphogen on-demand and to induce dentin differentiation solely within that specific spatial zone. This study demonstrates a significant advance in scaffold design to generate precise morphogen fields that can be used to develop in situ models to explore tissue differentiation and may ultimately be useful in engineering multi-lineage tissues in clinical dentistry.

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At the edge of translation – materials to program cells for directed differentiation

Cited by 16 publications

References 98 publications

Cell and tissue engineering for liver disease

Cell and tissue engineering for liver disease

Biophysical Approaches for Oral Wound Healing: Emphasis on Photobiomodulation

Multi-lineage MSC Differentiation via Engineered Morphogen Fields

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