Asynchronous cytoplast and karyoplast transplantation reveals that the cytoplasm determines the developmental fate of the nucleus in mouse oocytes

Cheng, Yong; Fan, Heng Yu; Wen, Duancheng; Tong, Chao; Zhu, Zi Yu; Lei, Lei; Sun, Qing; Chen, Da Yuan

doi:10.1002/mrd.10285

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…Immunoblotting showed that mitogenactivated protein kinase (MAPK) activity was high in MI and MII cytoplasts but not detected in GV 4 cytoplast. The results demonstrated that the cytoplsmic environment determines the behavior of asynchronous donors [1].…”

Section: Introductionmentioning

confidence: 90%

Supplementation of c-type natriuretic peptide during the whole in vitro growth period benefits the development of murine secondary follicles

Cao²,

Ruijiao³

et al. 2020

Preprint

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Background Supplementation of c-type natriuretic peptide (CNP) in the culture medium shortly before in vitro maturation (IVM) has been reported to be effective in delaying meiotic resumption of murine oocyte. The present study investigated the effect of CNP supplementation during the whole period of in vitro growth (IVG) on the development of murine secondary ovarian follicles.Methods Late secondary ovarian follicles isolated from ovaries of Kunming mice were cultured in vitro with and without supplementation of CNP. In experiment 1, CNP was supplemented at the early stage and the follicle development was evaluated. In experiment 2 and 3, CNP was supplemented during the whole period of IVG. In experiment 2, follicle development and oocyte maturity were evaluated. In group 3, follicle development and rate of cleaved embryos after in vitro fertilization (IVF) was assessed.Results In control group in all 3 experiments, granulosa cells migrated from within follicle and adhered to the plate at different degrees. The follicles flattened and could not reach antral stage. About 39.8% (39/98) of the oocytes ovulated nakedly. As no antral follicle was obtained, IVF was not performed in control group in experiment 3. In experiment group in all 3 experiments, no migration of guanulosa cells was observed and the follicles grew three-dimensionally. Ovulation of naked oocyte decreased substantially. The rate of antral stage follicle were 45% (18/40) in experiment 1. This parameter was 75.9% (44/58) in experiment 2 and 3 combined. In experiment 2, in preovulatory follicles without ovulation induction, oocytes at germinal vesicle (GV) stage and germinal vesicle breakdown (GVBD) stage were 87.5% (14/16) and 12.5% (2/16), respectively. In preovulatory follicles with ovulation induction, no GV stage oocyte was retrieved, oocytes at GVBD and metaphase II (MII) stage were 50% (8/16), respectively. In experiment 3, among 18 follicles cultured, 12 cumulus-oocyte complexes (COC) ovulated automatically after ovulation induction. Eleven oocytes were fertilized and cleaved. Compared with control groups, the follicle development assessed by naked oocyte ovulation and follicle stage (preantral follicle and antral follicle) in experiment groups were significantly superior (p<0.0001). CNP effectively maintained oocytes’ meiotic arrest and enhanced fertilization competency.Conclusions The supplementation of CNP in culture system of murine late secondary follicle during the whole period of IVG could sustain the 3-dimensional structure of follicle, increase the antral formation rate. As a result, the oocyte’s competency to be fertilized was greatly improved.

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Section: Introductionmentioning

confidence: 90%

Supplementation of c-type natriuretic peptide during the whole in vitro growth period benefits the development of murine secondary follicles

Cao²,

Ruijiao³

et al. 2020

Preprint

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“…ERK) and securin (a.k.a., pituitary tumor transforming gene 1, PTTG1) proteins was performed by western blotting as described (18). Briefly, oocytes were lysed in Laemmli lysis buffer (BioRad Laboratories, Hercules, CA).…”

Section: Methodsmentioning

confidence: 99%

Strain-specific spontaneous activation during mouse oocyte maturation

Cheng

Zhong

Latham

2012

Fertility and Sterility

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Objective To examine whether spontaneous oocyte activation is determined by genetic differences and interacted with culture environment. Design Experimental Study. Setting Temple University School of Medicine. Animals C57BL/6, DBA/2, C3H/HeJ, and A/J strains, along with reciprocal F1 hybrid female mice (5–6 weeks). Intervention(s) Immature oocytes from different mouse strains were collected and cultured in different maturation conditions including different serum, serum replacement, bovine serum albumin (BSA) and follicle stimulation hormone (FSH). Main Outcome Measure(s) The emission of first polar body, pronucleus formation, meiotic arrest, spontaneous activation, and expression of maturation regulators. Result(s) Oocytes from C57BL/6 mice display a high rate of delayed first meiotic division and spontaneous activation after the first meiotic division with in vitro maturation (IVM), and the second meiosis with in vivo maturation (VVM) following superovulation. Spontaneous activation with IVM is sensitive to culture environment. Oocytes spontaneously activated during the first meiotic division with IVM have unusual paired tetrad chromosomes with slight connections at centromeres, whereas oocytes activated in vivo display haploidization from the second meiosis. Spontaneous activation is also seen in F1 hybrid oocytes, indicating a dominant trait from C57BL/6. Delayed meiosis was associated with reduced cylcin B and securin expression. Conclusion(s) Both mouse strain and culture environment have a significant effect on the incidence of meiotic defects and spontaneous activation. Reduced expression of meiotic regulators may underlie this effect.

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“…Both oocytes, donor and recipient, must be in the same stage of maturation (Cheng et al, 2003a). This transfer can be basically performed at two stages of oocyte maturation, either between two metaphase I or between two metaphase II oocytes.…”

Section: Exchange Of Whole Chromosome Groupsmentioning

confidence: 99%

Mammalian Oocyte Therapies

Fulka

Mrazek

Fulka

et al. 2005

Cloning and Stem Cells

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In assisted human reproduction, the cytoplasm of oocytes recovered from follicles is often abnormal. Its lower quality, especially in older patients, may be responsible for certain chromosomal abnormalities or developmental arrest. Thus, the deficiency of some vital molecules, which are necessary for oocyte maturation, can be the cause of infertility in women. Moreover, mutated mitochondrial DNA (mtDNA) that is located in the oocyte cytoplasm might be transmitted to offspring. With the advance of new micromanipulation techniques like the oocyte nucleus replacement or cytoplasmic transfer, some of these abnormalities could be theoretically eliminated. In this review, we briefly discuss some of these approaches and their potential use in assisted human reproduction.

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Asynchronous cytoplast and karyoplast transplantation reveals that the cytoplasm determines the developmental fate of the nucleus in mouse oocytes

Cited by 15 publications

References 29 publications

Supplementation of c-type natriuretic peptide during the whole in vitro growth period benefits the development of murine secondary follicles

Supplementation of c-type natriuretic peptide during the whole in vitro growth period benefits the development of murine secondary follicles

Strain-specific spontaneous activation during mouse oocyte maturation

Mammalian Oocyte Therapies

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