Asymmetric Tug-of-War leads to Cooperative Transport of a Cargo by Multiple Kinesins

Tjioe, Marco; Shukla, Saurabh; Vaidya, Rohit; Troitskaia, Alice; Bookwalter, Carol S.; Trybus, Kathleen M.; Chemla, Yann R.; Selvin, Paul R.

doi:10.1101/544080

Cited by 2 publications

(2 citation statements)

References 51 publications

(60 reference statements)

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“…In the case of multi-motor cargos, we anticipate that kinesin motors are just as likely to get stuck at an obstacle as a single motor. However, the other motors driving the cargo may exert a force on the stuck motor, causing its rapid detachment and the continued forward motion of the cargo (Tjioe et al, 2019). In this process, new motors in the leading direction are recruited to the lattice while the motors in the trailing direction detach from the MT.…”

Section: Discussionmentioning

confidence: 99%

Kinesin and dynein use distinct mechanisms to bypass obstacles

Ferro

Can

Turner

et al. 2019

eLife

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Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro. Contrary to previous reports, we found that single mammalian dynein is highly capable of bypassing obstacles. Single human kinesin-1 motors fail to avoid obstacles, consistent with their inability to take sideways steps on to neighboring MT protofilaments. Kinesins overcome this limitation when working in teams, bypassing obstacles as effectively as multiple dyneins. Cargos driven by multiple kinesins or dyneins are also capable of rotating around the MT to bypass large obstacles. These results suggest that multiplicity of motors is required not only for transporting cargos over long distances and generating higher forces, but also for maneuvering cargos on obstacle-coated MT surfaces.

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Section: Discussionmentioning

confidence: 99%

Kinesin and dynein use distinct mechanisms to bypass obstacles

Ferro

Can

Turner

et al. 2019

eLife

View full text Add to dashboard Cite

show abstract

“…However, the other motors driving the cargo exert a force on the stuck motor, causing its rapid detachment and the continued forward motion of the cargo. 32 Multi-motor teamwork also proved beneficial for both types of motors when challenged by a large obstacle. Cargo beads driven by multiple kinesin or dynein motors were able to maneuver around a PDMS obstruction that blocked access to half of the MT surface.…”

Section: Discussionmentioning

confidence: 99%

Kinesin-1 and dynein use distinct mechanisms to bypass obstacles

Ferro

Can

Turner

et al. 2019

Preprint

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Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargos. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro. Contrary to previous reports, we found that mammalian dynein is highly capable of bypassing obstacles. Unlike dynein, single kinesin motors stall in the presence of obstacles, consistent with their inability to take sideways steps to neighboring protofilaments. Kinesins overcome this limitation when working in teams, bypassing obstacles as effectively as multiple dyneins. Cargos driven by multiple kinesin or dyneins are also capable of rotating around the MT to bypass large obstacles. These results suggest that multiplicity of motors is required not only for transporting cargos over long distances and generating higher forces, but also for maneuvering of the cargos on obstacle-coated MT surfaces.

show abstract

Asymmetric Tug-of-War leads to Cooperative Transport of a Cargo by Multiple Kinesins

Cited by 2 publications

References 51 publications

Kinesin and dynein use distinct mechanisms to bypass obstacles

Kinesin and dynein use distinct mechanisms to bypass obstacles

Kinesin-1 and dynein use distinct mechanisms to bypass obstacles

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