Asymmetric patterns in the cranial skeleton of zebrafish (Danio rerio) exposed to sodium pentachlorophenate at different embryonic developmental stages

López‐Romero, Faviel A.; Zúñiga, Gerardo; Martínez‐Jerónimo, Fernando

doi:10.1016/j.ecoenv.2012.06.008

Cited by 16 publications

(12 citation statements)

References 46 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further negative results come from two datasets of wings from Chironomus midges [116], whereas other studies found mixed results for wings and larval mouthparts [103,130,131]. Some studies, in a variety of species, have found mixed results, with some structures or subgroups yielding significant results, but others not [132][133][134][135][136][137][138]. Because these studies with negative results are relatively few and some are based on relatively small samples, the question arises whether negative results really indicate the absence of directional asymmetry or whether they result from limitations of statistical power.…”

Section: Directional Asymmetrymentioning

confidence: 59%

“…Additional experiments demonstrated that alkaloids in the cactus tissue can cause increased fluctuating asymmetry and abnormal wing phenotypes [119]. Not all toxins affect fluctuating asymmetry of shape: an experiment showed that even applications of sodium pentachlorophenate that were sufficiently severe to suppress cranial growth of zebrafish did not produce increased fluctuating asymmetry of shape [137]. Administering TCDD had no effect on fluctuating asymmetry of shape in mouse mandibles, but affected average shape [43], whereas the same toxin both increased fluctuating asymmetry and changed the average shape of molar teeth [373].…”

Section: Developmental Instability Of Shape In Relation To Stress Andmentioning

confidence: 99%

“…Also, the asymmetry scores are not in units of Procrustes distance, and therefore are not easily related to other measures of shape variation in the data. The asymmetry measure using Mahalanobis distance is used in an increasing number of studies, comparing levels of fluctuating asymmetry between groups or correlating it with factors that might influence developmental instability, both for structures with matching symmetry [77,124] and with object symmetry [90,98,108,137,253].…”

Section: Individual Measures Of Fluctuating Asymmetry Of Shapementioning

confidence: 99%

See 2 more Smart Citations

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

2015

View full text Add to dashboard Cite

Approximately two decades after the first pioneering analyses, the study of shape asymmetry with the methods of geometric morphometrics has matured and is a burgeoning field. New technology for data collection and new methods and software for analysis are widely available and have led to numerous applications in plants and animals, including humans. This review summarizes the concepts and morphometric methods for studying asymmetry of shape and size. After a summary of mathematical and biological concepts of symmetry and asymmetry, a section follows that explains the methods of geometric morphometrics and how they can be used to analyze asymmetry of biological structures. Geometric morphometric analyses not only tell how much asymmetry there is, but also provide information about the patterns of covariation in the structure under study. Such patterns of covariation in fluctuating asymmetry can provide valuable insight about the developmental basis of morphological integration, and have become important tools for evolutionary developmental biology. The genetic basis of fluctuating asymmetry has been studied from empirical and theoretical viewpoints, but serious challenges remain in this area. There are many promising areas for further research that are only little explored at present.

show abstract

Section: Directional Asymmetrymentioning

confidence: 59%

Section: Developmental Instability Of Shape In Relation To Stress Andmentioning

confidence: 99%

Section: Individual Measures Of Fluctuating Asymmetry Of Shapementioning

confidence: 99%

See 1 more Smart Citation

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

2015

View full text Add to dashboard Cite

show abstract

“…This assay has since become the established zebrafish embryo test and is widely used in chemical screening as well as being recommended by the Organisation for Economic Co-operation and Development (OECD) (McGrath and Li 2008). Since insecticides are known to pose a potential threat to aquatic animals, various studies have utilized zebrafish embryos to evaluate toxicity related to these insecticides (Chandrasekar et al 2011;Lopez-Romero et al 2012;Mizell and Romig 1997;Osterauer and Kohler 2008;Pandey and Guo 2014;Shi et al 2011;Xu et al 2014;Yang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Larvicidal activity of endophytic fungal extract of Cochliobolus spicifer (Pleosporales: Pleosporaceae) on Aedes caspius and Culex pipiens (Diptera: Culicidae)

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Ventral images of the branchial arches were captured using a camera-mounted microscope, similar to the methods used by Lopez-Romero and co-workers [12]. Subsets of specimens from each of the three experimental rounds were included in the data collection for a total sample size of 35 (70% O 2 ,n=19; 50% O 2 ,n=9; normal controls, n=7).…”

Section: Specimen Preparation and Data Collectionmentioning

confidence: 99%

Hypoxic conditions alter developing branchial arch-derived structures in zebrafish

Parsons¹,

Weinberg²,

Tsang³

et al. 2014

d3000

View full text Add to dashboard Cite

Background: Previous epidemiological findings have implicated hypoxia as a risk factor for craniofacial defects including cleft lip, microtia and branchial arch anomalies. This study tests the hypothesis that hypoxic exposure results in craniofacial shape variation in a zebrafish model. Methods: Three sets of zebrafish embryos were raised in uniform conditions with the exception of dissolved oxygen level. At 24 hours past fertilization (hpf) embryos were placed in hypoxic conditions (70% or 50% dissolved oxygen tank water) and compared to unexposed control embryos. After 24 hours of exposure to hypoxia, the embryos were incubated under normoxia. Larvae were collected at 5 days post fertilization (dpf) and stained for cartilage. Images were taken of each specimen and subsequently landmarked to capture viscerocranial morphology. A geometric morphometric analysis was performed to compare shape variation across groups. Results: The mean branchial arch shape of each exposure group was significantly different from controls (p<0.001). Principal components analysis revealed a clear separation of the three groups, with controls at one end of the shape spectrum, the 50% hypoxia group at the other end, and the 70% hypoxia group spanning the variation in between. Conclusions: This experiment shows that hypoxia exposure at 24hpf is capable of affecting craniofacial shape in a dose-dependent manner. These results may have implications not only for high altitude fetal health, but other environments, behaviors and genes that affect fetal oxygen delivery.

show abstract

Asymmetric patterns in the cranial skeleton of zebrafish (Danio rerio) exposed to sodium pentachlorophenate at different embryonic developmental stages

Cited by 16 publications

References 46 publications

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

Larvicidal activity of endophytic fungal extract of Cochliobolus spicifer (Pleosporales: Pleosporaceae) on Aedes caspius and Culex pipiens (Diptera: Culicidae)

Hypoxic conditions alter developing branchial arch-derived structures in zebrafish

Contact Info

Product

Resources

About