Asymmetric Effects of Acute Hemiovariectomy on Steroid Hormone Secretion by the In Situ Ovary

Barco, Ana Isabel; Flores, Angélica; Chavira, Roberto; Damián-Matsumura, Pablo; Domı́nguez, Roberto; Cruz, Maria Antonieta

doi:10.1385/endo:21:3:209

Cited by 22 publications

(30 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensitivity of the left and right ovaries is also different. This is in agreement with the findings of other authors who have shown an asymmetry in the behaviour of the left ovary in relation to the right one according to the characteristics of the neural stimulus applied (Dominguez et al 1989, Barco et al 2003, Morán et al 2005. Besides, the ganglion presence in the preparation and good conservation of its structure is kept until the end of the incubation period, which shows the excellent viability of the incubation system.…”

Section: Discussionsupporting

confidence: 92%

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Orozco¹,

Sosa²,

Fillipa³

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

The axons that constitute the ovarian nervous plexus originate mostly in the principal neurons of the superior mesenteric ganglion (SMG) that is part of the sympathetic ganglionic chain and exhibits cholinergic receptors. In order to observe the effect of acetylcholine, the main neurotransmitter in the ganglionic transmission, the purpose of the present work was: first, to standardize an integrated ex vivo superior mesenteric ganglion-ovarian nervous plexus-ovary (SMG-ONP-O) system in oestrus day rats; secondly, to determine if the ganglionic cholinergic stimulus modifies the release of nitric oxide and steroids in the ovary compartment in the absence of humoral factors; and thirdly, to investigate if there are differences in the responses between the left and right ovaries caused by the neural stimulus. The ex vivo experimental left and right systems were developed and standardized. The systems were incubated in Krebs-Ringer phosphate buffer in a Dubnoff metabolic shaker. The progesterone release was determined to standardize the incubation times, obtaining different responses between the left and right systems, which shows that both systems have their own autonomic tone. Non-specific stimulation with KCl in the ganglion compartment provoked different responses in terms of release of progesterone and oestradiol. Progesterone decreased in the left and right systems.However, oestradiol diminished at short times and increased at 60 and 120 min in the left ovary, whereas it increases at 30 and 60 min in the right ovary. These different responses show the sensitivity and viability of both systems. When acetylcholine was used in the ganglion compartment, the release of nitric oxide, progesterone, androstenedione and oestradiol was evaluated. The liberation of nitrite increased at 15, 30 and 60 min in the left system and decreased in the right system at 120 min. Progesterone showed a decrease in its release at 15, 30 and 120 min and androstenedione at 15 min in the left ovary compartment. In the right ovary, only progesterone decreased in relation to the control at 120 min while androstenedione did not show significant changes. Oestradiol showed an increase in the left ovary compartment at all the studied times, while in the right ovary it did not show any changes. These results indicate that the neural stimulus from the superior mesenteric ganglion through the ovarian nervous plexus is one of the factors modulating the secretory activity of the ovarian steroids and nitric oxide. The system is viable and also shows a different sensitivity of the left ovary in relation to the right one at least in this cycle stage, characterized by marked irrigation and profound structural changes in the ovary.

show abstract

Section: Discussionsupporting

confidence: 92%

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Orozco¹,

Sosa²,

Fillipa³

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…In the rat the left ovary releases more ova than the right-side one . The left ovary has been reported to have a greater capacity to secrete progesterone (Barco et al, 2003;Flores et al, 2005) and testosterone (Flores et al, 2006) in response to hemicastration. On the basis of the asymmetry in the number of ova shed and in the response of the left and right ovary to acute hemiovariectomy, asymmetry in the innervation of the left and right ovary has been postulated.…”

Section: Discussionmentioning

confidence: 99%

“…Asymmetry of the rodent ovary includes difference in the venous drainage and functional asymmetry of the gonad in response to acute hemicastration (Barco et al, 2003;Flores et al, 2005) or to transection of the nerves supplying the gland (Chavez and Dominguez, 1994;Chavez et al, 1987Chavez et al, , 1989. Concerning asymmetry at the level of cerebral structures involved in the control of ovarian functions, observations indicate both biochemical and functional asymmetry of hypothalamic and temporolimbic structures (see for review Gerendai and Halász, 1997).…”

Section: Introductionmentioning

confidence: 99%

Predominance of supraspinal innervation of the left ovary

Tóth

Wiesel

Boldogköi

et al. 2007

Microscopy Res & Technique

View full text Add to dashboard Cite

In our previous studies using the viral transneuronal tracing technique we demonstrated the spinal and supraspinal components of the ovarian innervation. Since increasing number of data indicate the presence of morphological and functional laterality in the control of gonadal functions, we aimed to investigate whether cerebral structures trans-synaptically involved in the innervation of the ovary exhibit asymmetry or not. In one of the studies the left or the right ovary was injected with the red fluorescent protein expressing pseudorabies virus and the number of infected "red" autofluorescent neurons from the right and the left ovary was compared. In another study in order to have distinct labeling of cell groups connected with the right- and left-sided ovary in the same animal, a dual viral labeling was applied. The left- and right-sided ovary were inoculated with genetically engineered pseudorabies virus expressing a red fluorescent protein or a green fluorescent protein gene. Viral infection of brain nuclei including the dorsal vagal nucleus, caudal raphe nuclei, A5 noradrenergic cell group, hypothalamic paraventricular nucleus, from the left ovary in each case was enhanced when compared with labeling from the right gonad. Data suggest a predominance in the supraspinal innervation of the left ovary.

show abstract

“…Previously, we showed that compared to cyclic sham-operated animals, acute extirpation of the left or right ovary results in changes in estradiol, progesterone and testosterone serum levels, and that these changes depend on the hormone studied and the day of the cycle when surgery was performed; indicating an asymmetric ability for hormone release by the ovaries [36-38]. Gerendai et al [39] did not observe such effects when performing ULO to adult rats nine days after surgery.…”

Section: Discussionmentioning

confidence: 99%

Ipsilateral vagotomy to unilaterally ovariectomized pre-pubertal rats modifies compensatory ovarian responses

Morales¹,

Ricardo²,

Bolanos³

et al. 2007

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

The present study evaluates the participation of the vagus nerve in pre-pubertal rats with unilateral ovariectomy on puberty onset, and on progesterone, testosterone and estradiol serum levels, and the compensatory responses of the ovary. Unilateral vagotomy did not modify the onset of puberty in unilaterally ovariectomized rats. Ovulation rates of animals with the left vagus nerve sectioned and the left ovary in-situ was lower than in rats with only unilateral ovariectomy. Sectioning the left vagus to 32-day old rats with the left ovary in-situ resulted in lower compensatory ovarian hypertrophy than in rats with right unilateral ovariectomy. Twenty-eight or 32-day old animals with sectioning of the right vagus nerve and the right ovary in situ showed higher compensatory ovulation. Twenty-eight -day old rats with the right ovary in situ had higher progesterone and testosterone levels than animals of the same age with the left ovary in-situ. Compared to animals with the right ovary in situ, animals treated at 32-days of age, sectioning the ipsi-lateral vagus nerve resulted in higher progesterone levels. Higher progesterone levels were observed in 28- and 32 days old rats with the left ovary in situ and left vagus nerve sectioned. Thirty-two day old animals with the right ovary in situ and right vagus nerve sectioned had higher progesterone levels than rats of the same age with the left ovary in situ and left vagus nerve sectioned. Left vagotomy to 28-day old rats with the left ovary in situ resulted in higher testosterone levels, a reverse response to that observed in animals with sectioning of the right vagus and the right ovary in situ. Thirty-two day old rats with the left ovary in situ and left vagus nerve sectioned showed lower testosterone levels than animals without vagotomy and with the left ovary in situ.Twenty-eight -day old animals with the left vagus sectioned and left ovary in situ had lower estradiol serum levels than rats without unilateral vagotomy, a response similar to that observed in 32-day old rats with the right ovary in situ and right vagus nerve sectioned. Present results suggest an asymmetric regulation of steroid hormones secretion by the vagus nerve innervations in animals with unilateral ovariectomy, and those differences in testosterone serum levels observed are associated to the ovary remaining in-situ, vagal innervation and age when the animals were treated.

show abstract

Asymmetric Effects of Acute Hemiovariectomy on Steroid Hormone Secretion by the In Situ Ovary

Cited by 22 publications

References 23 publications

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Predominance of supraspinal innervation of the left ovary

Ipsilateral vagotomy to unilaterally ovariectomized pre-pubertal rats modifies compensatory ovarian responses

Contact Info

Product

Resources

About