Asymmetric division and cell-fate determination in developing pollen

Twell, David; Park, Soon Ki; Lalanne, Éric

doi:10.1016/s1360-1385(98)01277-1

Cited by 157 publications

(93 citation statements)

References 35 publications

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“…This arrangement is accomplished soon after meiosis, when an asymmetric mitotic division produces a large cell that engulfs its diminutive sister, the generative cell (Twell et al, 1998;Yang and Sundaresan, 2000). Subsequently, the generative cell undergoes a second mitosis to form the second sperm cell required for double fertilization; ''tricellular'' pollen completes this division before it is released from the anther, whereas ''bicellular'' pollen undergoes this division only later, within the elongating pollen tube.…”

Section: Overview Of Structuresmentioning

confidence: 99%

Pollen and Stigma Structure and Function: The Role of Diversity in Pollination

Edlund

Swanson

Preuss

2004

THE PLANT CELL ONLINE

485

431

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Section: Overview Of Structuresmentioning

confidence: 99%

Pollen and Stigma Structure and Function: The Role of Diversity in Pollination

Edlund

Swanson

Preuss

2004

THE PLANT CELL ONLINE

485

431

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Section: Introductionmentioning

confidence: 99%

“…In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction.…”

mentioning

confidence: 99%

A reference map of the Arabidopsis thaliana mature pollen proteome

Noir

Bräutigam

Colby

et al. 2005

Biochemical and Biophysical Research Communications

135

145

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The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome. Ó 2005 Elsevier Inc. All rights reserved.Keywords: Arabidopsis thaliana; Male gametophyte; Mass spectrometry; Mature pollen; Proteome; Two-dimensional gel electrophoresis In higher plants, development of the male gametophyte is a well-programmed and elaborate process. Male sporogenesis begins with the division of a diploid sporophytic cell giving rise to the anther wall of the stamen and the sporogenic cells. The latter cells then undergo several mitoses to differentiate into pollen mother cells. Subsequently, each diploid pollen mother cell forms a tetrad of haploid microspores via a round of DNA replication followed by two consecutive meiotic divisions. Each uninucleate microspore then undergoes an asymmetric mitotic division forming a large vegetative cell and a smaller generative cell (i.e., the bicellular pollen stage). In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction. In addition, it represents a very useful model for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth [5][6][7].The availability of the full genome sequence of Arabidopsis [8] has made genome-wide, microarray-based analyses of the male gametophyte transcriptome of this model plant possible [9][10][11][12]. 13,977 male gametophyte-expressed mRNAs were recently identified using the ATH1 Genome Array, which cover...

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“…Male gametophytic division at pollen mitosis I (PMI) is highly asymmetric and involves the production of a unique hemispherical cell wall that encloses the generative nucleus. This gives rise to two cells with different structures and fates, the vegetative cell (VC) and the generative cell (GC) (Tanaka 1997;Twell et al 1998). Cytokinesis at PMI displays several unique features compared to somatic cytokinesis.…”

Section: Introductionmentioning

confidence: 99%

Gemini pollen 2, a male and female gametophytic cytokinesis defective mutation

Park

Rahman

et al. 2004

Sex Plant Reprod

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Gametophytic cytokinesis is essential for the development and function of the male and female gametophytes. We have previously described the isolation and characterisation of the gemini pollen 1 (gem1) that acts gametophytically to disturb asymmetric division and cytokinesis at pollen mitosis I in Arabidopsis. Here we describe the genetic and cytological analysis of an independent gametophytic mutant, gem2, with similar characteristics to gem1, but which maps to a different genetic locus. gem2 shows reduced genetic transmission through both male and female gametes and leads to the production of divided or twin-celled pollen. Developmental analysis revealed that gem2 does not affect karyokinesis at pollen mitosis I, but leads to repositioning of the cell plate and partial or complete failure of cytokinesis, resulting in symmetrical divisions or binucleate pollen grains respectively. Symmetrical divisions lead to altered pollen cell fate with both sister cells displaying vegetative cell fate. Moreover, we demonstrate that the predominant female defect in gem2 is a lack of cellularization of the embryo sac during megagametogenesis. GEM2 therefore defines an independent genetic locus that is involved in the correct specification of both male and female gametophytic cytokinesis.

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Asymmetric division and cell-fate determination in developing pollen

Cited by 157 publications

References 35 publications

Pollen and Stigma Structure and Function: The Role of Diversity in Pollination

Pollen and Stigma Structure and Function: The Role of Diversity in Pollination

A reference map of the Arabidopsis thaliana mature pollen proteome

Gemini pollen 2, a male and female gametophytic cytokinesis defective mutation

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