Asymmetric Distribution of Anionic Phospholipids in Supported Lipid Bilayers

Stanglmaier, S.; Hertrich, Samira; Fritz, K.; Moulin, Jean‐François; Haese, Martin; Rädler, Joachim O.; Nickel, Bert

doi:10.1021/la3019887

Cited by 37 publications

(45 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies showed unique substrate-induced phenomena of SLBs, which do not occur in the free-standing membranes; for example, decoupled phase transition [34,35,36,37] and asymmetric molecular distribution between the upper and lower leaflet of a SLB [38,39,40]. Understanding the interaction between solid substrates and lipid bilayers are important to realize artificial cell membrane systems on functional solid devices avoiding the denaturing of lipids and proteins and, furthermore, to control the bilayer membranes with functional substrates, because cell membranes are laterally heterogeneous and vertically asymmetric.…”

Section: Introductionmentioning

confidence: 99%

Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers

2012

View full text Add to dashboard Cite

Supported lipid bilayers are artificial lipid bilayer membranes existing at the interface between solid substrates and aqueous solution. Surface structures and properties of the solid substrates affect the formation process, fluidity, two-dimensional structure and chemical activity of supported lipid bilayers, through the 1–2 nm thick water layer between the substrate and bilayer membrane. Even on SiO2/Si and mica surfaces, which are flat and biologically inert, and most widely used as the substrates for the supported lipid bilayers, cause differences in the structure and properties of the supported membranes. In this review, I summarize several examples of the effects of substrate structures and properties on an atomic and nanometer scales on the solid-supported lipid bilayers, including our recent reports.

show abstract

Section: Introductionmentioning

confidence: 99%

Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers

2012

View full text Add to dashboard Cite

show abstract

“…48 Lipid asymmetry between the two leaflets in mixed bilayer systems was recently reported for bilayers deposited by osmotic shock from 3:1 POPC/POPS vesicles where the initial vesicle adsorption took place in buffer solutions containing 1.1 M NaCl. 49 For the bilayers in Figure 4, simultaneous fitting of the SLD values in the inner and outer Table 1 (fitting parameters in Table S1 of the Supporting Information). The reflectivity was measured for three degrees of solvent deuteration: D 2 O (red circles, top curve), CMSi (green triangles, bottom curve), and H 2 O (blue squares, middle curve).…”

mentioning

confidence: 99%

Adsorption of α-Synuclein to Supported Lipid Bilayers: Positioning and Role of Electrostatics

Hellstrand

Grey

Ainalem

et al. 2013

ACS Chem. Neurosci.

102

View full text Add to dashboard Cite

An amyloid form of the protein α-synuclein is the major component of the intraneuronal inclusions called Lewy bodies, which are the neuropathological hallmark of Parkinson's disease (PD). α-Synuclein is known to associate with anionic lipid membranes, and interactions between aggregating α-synuclein and cellular membranes are thought to be important for PD pathology. We have studied the molecular determinants for adsorption of monomeric α-synuclein to planar model lipid membranes composed of zwitterionic phosphatidylcholine alone or in a mixture with anionic phosphatidylserine (relevant for plasma membranes) or anionic cardiolipin (relevant for mitochondrial membranes). We studied the adsorption of the protein to supported bilayers, the position of the protein within and outside the bilayer, and structural changes in the model membranes using two complementary techniquesquartz crystal microbalance with dissipation monitoring, and neutron reflectometry. We found that the interaction and adsorbed conformation depend on membrane charge, protein charge, and electrostatic screening. The results imply that α-synuclein adsorbs in the headgroup region of anionic lipid bilayers with extensions into the bulk but does not penetrate deeply into or across the hydrophobic acyl chain region. The adsorption to anionic bilayers leads to a small perturbation of the acyl chain packing that is independent of anionic headgroup identity. We also explored the effect of changing the area per headgroup in the lipid bilayer by comparing model systems with different degrees of acyl chain saturation. An increase in area per lipid headgroup leads to an increase in the level of α-synuclein adsorption with a reduced water content in the acyl chain layer. In conclusion, the association of α-synuclein to membranes and its adsorbed conformation are of electrostatic origin, combined with van der Waals interactions, but with a very weak correlation to the molecular structure of the anionic lipid headgroup. The perturbation of the acyl chain packing upon monomeric protein adsorption favors association with unsaturated phospholipids preferentially found in the neuronal membrane.

show abstract

“…The local environments of bilayer leaflets frequently differ: examples include the different leaflet solvent environments in vivo, or preferential substrate interactions with one species [5,[21][22][23]. The simplest effects can be modelled with an excess free energy per lipid −ζ for findAll rights reserved.…”

Section: Effect Of An External Fieldmentioning

confidence: 99%

Effects of passive phospholipid flip-flop and asymmetric external fields on bilayer phase equilibria

Williamson

Olmsted

2018

Preprint

View full text Add to dashboard Cite

Compositional asymmetry between the leaflets of bilayer membranes is known to couple strongly to their phase behaviour, in addition to having important effects on, e.g., mechanical properties and protein activity. We address how phase behaviour is affected by passive phospholipid flip-flop, such that the compositional asymmetry is not fixed. We predict transitions from "pre flip-flop" behaviour to a restricted set of phase equilibria that can persist in the presence of passive flipflop. Surprisingly, such states are not necessarily symmetric. We further account for external symmetry-breaking, such as a preferential substrate interaction, and show how this can stabilise strongly asymmetric equilibrium states. Our theory explains several experimental observations of flip-flop mediated changes in phase behaviour, and shows how domain formation and compositional asymmetry can be controlled in concert, by manipulating passive flip-flop rates and applying external fields.

show abstract

Asymmetric Distribution of Anionic Phospholipids in Supported Lipid Bilayers

Cited by 37 publications

References 24 publications

Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers

Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers

Adsorption of α-Synuclein to Supported Lipid Bilayers: Positioning and Role of Electrostatics

Effects of passive phospholipid flip-flop and asymmetric external fields on bilayer phase equilibria

Contact Info

Product

Resources

About