Asymmetric Collagen/chitosan Membrane Containing Minocycline-loaded Chitosan Nanoparticles for Guided Bone Regeneration

Abstract

Infections caused by pathogens colonization at wound sites in the process of bone healing are considered as one of the major reasons for the failure of guided bone regeneration (GBR). The objective of this study was to prepare a novel asymmetric collagen/chitosan GBR membrane containing minocycline-loaded chitosan nanoparticles. The morphologies of the membranes and nanoparticles were observed by SEM and TEM, respectively. The characterization and biocompatibility of the membranes was evaluated. The effect of the membrane on bone regeneration was assessed using the critical-size at cranial defect model. TEM images showed the spherical morphology of the nanoparticles. The results of SEM indicated that the asymmetric membrane contained a dense collagen layer and a loose chitosan layer. An in vitro experiment showed that the membrane can inhibit bacterial growth and promote osteoblasts and fibroblasts growth. The membrane showed the ability to promote angiogenesis and enhance bone regeneration in vivo. An asymmetric collagen/chitosan GBR membrane can be fabricated by loading minocycline encapsulated chitosan nanoparticles, and shows satisfactory biocompatibility and barrier function, which enhances bone regeneration. Therefore, this antibacterial GBR membrane is a promising therapeutic approach to prevent infection and guide bone regeneration.

Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide

“…An ideal GBR membrane should be biodegradable and biocompatible and should exhibit space-maintaining and bone regeneration abilities as well as clinical manageability. 35 At present, several composites of degradable membranes such as polytetrafluoroethylene, collagen membrane, HA/poly l-lactide, and poly l-lactide/alginate have been developed. 5,36 However, these membranes lack the characteristics of GBR membrane, including the bone regeneration ability.…”

Antimicrobial and Immunomodulatory Surface‐Functionalized Electrospun Membranes for Bone Regeneration

“…GBR membranes must fulfil several criteria such as biocompatibility, space maintaining capacities, and the preservation of occlusive properties for not only the tissue regeneration time period but also the whole duration of the wound maturation and remodeling process . In addition to these physical criteria, bioactivity can be considered a desirable feature of GBR membranes in order to initiate and enhance bone formation . Medical devices fabricated by solution electrospinning are potential candidates for application in GBR and our group has developed and characterized a medical grade polycaprolactone (mPCL) membrane possessing many of these aforementioned properties such as flexibility, space maintenance, micro to nanofibers diameter, small pore size that imparts occlusive properties, and a highly interconnected porous network favorable for long term tissue infiltration and integration .…”

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