Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid

Zeng, Songjun; Wu, Kunlin; Silva, Jaime A. Teixeira da; Zhang, Jianxia; Chen, Zhilin; Xia, Nian‐He; Duan, Jun

doi:10.1016/j.scienta.2012.02.026

Cited by 113 publications

(125 citation statements)

References 33 publications

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“…Presently, efforts have been made to devise a protocol to raise seedlings by achieving optimum seed germination, multiplying the cultures and subsequently acclimatizing the plantlets under in vivo environment. In vitro culture techniques provide a practical system for clonal, rapid mass multiplication of rare, endangered, horticulturally, and therapeutically important genotypes [8][9][10][11][12][13]. Asymbiotic seed germination is an important technique for rescuing and propagating a large number of orchid species and hybrids.…”

Section: Digital Signaturementioning

confidence: 99%

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Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Kaur¹,

Bhutani²

2016

Acta Soc Bot Pol

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The present study was intended to facilitate ex situ conservation of Paphiopedilum venustum, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g L )] growth supplements. Peptone at 1.0 g L −1 was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.

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Section: Digital Signaturementioning

confidence: 99%

“…Asymbiotic seed germination is an important technique for rescuing and propagating a large number of orchid species and hybrids. Few reports of asymbiotic seed germination of Paphiopedilum species are available [10,[14][15][16][17].…”

Section: Digital Signaturementioning

confidence: 99%

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Kaur¹,

Bhutani²

2016

Acta Soc Bot Pol

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“…However, is necessary to evaluate the response of each species to different culture media available for this technique of cultivation, as this response is not homogeneous (Chu, Tavares, Kanashiro, Giampaoli & Yokota, 2010;Zeng, Wu, Silva, Zhang, Chen, Xia & Duan, 2012). These culture media should enriched with supplements that promote the survival, growth and development of the explants.…”

Section: Introductionmentioning

confidence: 99%

In vitro germination and growth of Vriesea incurvata Gaudich. (Bromeliaceae)

2018

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Vriesea incurvata in the natural environment shows some difficulties associated to the low seed germination capacity. Aiming to provide basis for the seedlings production, the results of the seeds germination percentage and the initial growth of V. incurvata seedlings in vitro conditions in different culture media, are reported. Completely randomized design was comprised of eight treatments and eight replications. The treatments were as culture media: MS (Murashige & Skoog); MS½; KC (Knudson); KC½; MS + activated carbon (AC); MS½ + AC; KC + AC; and KC½ + AC. The germination percentage was calculated from the division between the number of seeds with primary root extrusion by the total number of sowed seeds. The initial growth was evaluated considering the values of total fresh biomass, percentage of normal and dead seedlings, number and roots length, stem length, number of leaves and percentage of chlorotic, necrotic and dead leaves, respectively. All the cultures promoted high germination percentages (> 82.8%). In MS and MS½ medium, it was evidenced the highest percentage of normal seedlings, the highest values of fresh biomass production, stem growth and number of leaves. KC and KC½ medium also promoted highest percentages of normal seedlings and low percentages of necrotic and dead leaves. The addition of activated carbon in the culture media was unfavorable to promote the growth of seedlings. This suggests that MS and MS½ are the most suitable culture media for the production of Vriesea incurvata in vitro.

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“…The high germination rate was probably due to the seeds was harvested during the right time. Zeng et al (2012), stated that fruit harvesting during the right moment will cause a high germination rate because in this period the embryo fully formed and the testa might not be lignified, allowing it to be permeable to water and nutrients. This is probably because of efficient protein mobilization during rehydration and an undeveloped embryonic envelope.…”

mentioning

confidence: 99%

“…This is probably because of efficient protein mobilization during rehydration and an undeveloped embryonic envelope. It is also because of the cuticular layer was not fully formed and suspensor cells were vacuolated, thereby enabling functional nutrition uptake (Zeng et al 2012;Zhang et al 2013). …”

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Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

Rahayu

2016

Nusantara Biosci

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Abstract. Rahayu EMD. 2016. Handling and propagation of Dendrobium 'Iriana Jokowi' in Bogor Botanic Gardens, Indonesia. Nusantara Bioscience 8: 258-263. Dendrobium 'Iriana Jokowi' is a new hybrid in Indonesia which has not being mass propagated yet. Mass propagation is necessary in order to ensure seedling availability. The orchid has been grown in the Orchids Greenhouse of Bogor Botanic Gardens since July 30 th 2015. Handling and care for Dendrobium 'Iriana Jokowi', includes watering, provision of fertilizer, fungicides, and insecticides. Propagation has already been done is using seed culture. The seeds of Dendrobium 'Iriana Jokowi' were sown in four type of culture media, which are modified Vacin & Went (VW), modified Hyponex (HS), modified Knudson's C (KC), and Knudson's C (KCA). The seeds were able to germinate in all four tested media. The highest germination rate in the first 3 months after sowing (3 MAS) were found in KCA media, which are 93.21%. Germinated seed were then subcultured to four type of media in order to induce the growth of leaves and roots. The media used were T1A, T1V, HS, and KCA. After 5 months of planting (5 MAP), result showed that all four media were able to provide significant effect to the number of leaves and roots. The highest number of average leaves grown was 4.8 and found in T1A media. On the other hand, the highest number of average of roots grown was found in T1V media (4,2 roots).

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Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid

Cited by 113 publications

References 33 publications

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

In vitro germination and growth of Vriesea incurvata Gaudich. (Bromeliaceae)

Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

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