Astroglial Elements in the Suprachiasmatic Nucleus of the Golden Hamster and the Rat. Comparative Morphological Study Using Immunocytochemistry.

Inoue, Kenzo; Tamada, Yoshitaka; Munekawa, Katsuhiko; Hayashi, Seiji; Iijima, Norio; Ishihara, Akihiko; Ikeda, Teru; Tanaka, Masaki

doi:10.1267/ahc.33.95

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…The SDH activities of~200 fibers from each muscle were determined on digitized images of the stained sections. Tissue sections were digitized as grayscale images, and the value of the SDH staining intensity was expressed as an optical density (OD) value on the above-mentioned image processing system [15,17,19]. Each pixel was quantified as one of 256 gray levels.…”

Section: Succinate Dehydrogenase (Sdh) Activitymentioning

confidence: 99%

Growth-Related Changes in Skeletal Muscle Fiber Type and Insulin Resistance in Diabetic Otsuka Long-Evans Tokushima Fatty Rats.

Yasuda

Ishihara

Adachi

et al. 2001

Acta Histochem. Cytochem

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We investigated whether a relationship exists between the fiber type distribution in skeletal muscles and the insulin resistance of different ages of diabetic rats. The fiber type distributions of the slow soleus and fast plantaris muscles in 5-, 9-, and 21-week-old male Otsuka LongEvans Tokushima Fatty (OLETF) rats as an animal model of spontaneous type 2 diabetes mellitus were examined and compared with those in age-matched nondiabetic Long-Evans Tokushima Otsuka (LETO) rats. In the soleus muscle, a higher percentage of type I fibers was observed in the 9-and 21-week-old OLETF rats compared with the age-matched LETO rats, and there were no type IIA fibers remaining in the soleus muscle of the OLETF rats. In the plantaris muscle, a lower percentage of type IIA fibers and a higher percentage of type IIB fibers were observed in the 9-and 21-week-old OLETF rats compared with the agematched LETO rats. In addition, a lower percentage of type I fibers in the plantaris muscle was observed in the 9-weekold OLETF rats compared with the agematched LETO rats, and there were no type I fibers in the plantaris muscle of the 21-week-old OLETF rats. All 20-weekold OLETF rats showed serum insulin concentrations of more than 20 ng/ml. The present study suggests that insulin resistance and impaired glucose tolerance from altered muscle fiber type are linked to the cause of diabetes.

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Section: Succinate Dehydrogenase (Sdh) Activitymentioning

confidence: 99%

Growth-Related Changes in Skeletal Muscle Fiber Type and Insulin Resistance in Diabetic Otsuka Long-Evans Tokushima Fatty Rats.

Yasuda

Ishihara

Adachi

et al. 2001

Acta Histochem. Cytochem

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“…To quantify the MOR immunoreactivity in both groups, we employed a computer-assisted analyzing system. The MOR immunoreactivity was measured as the optical density per area (O.D./mm 2 ) using the NIH image software in the area where MOR neurons were distributed [7]. The O.D.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Foot Hyperalgesia after Thoracic Burn Injury. Histochemical, Behavioral and Pharmacological Studies.

Ueda

Hirose

Takei

et al. 2001

Acta Histochem. Cytochem

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Using behavioral, immunohistochemical and pharmacological studies, we report here that large thoracic burn injuries remotely induce hindpaw hyperalgesia during the healing stage. During 2-3 weeks after thoracic burn injury when the skin was regenerating from the wound, we observed by formalin test that the number of flinching behaviors significantly increased and simultaneously we observed by von Frey test that rats developed mechano-hyperalgesia in the foot. In the dorsal horn of the lumbar spinal cord in burn injured rats, c-Fos expression was significantly augmented after plantar formalin injection. The expression of m-opioid receptor in burn injured rats was significantly decreased compared with that in sham operated rats. The expression of substance P and CGRP in the lumbar dorsal horn was not different between burn and sham operated animals. We also observed that intrathecal administration of glutamate receptor antagonists (MK801 and CNQX) but not cyclooxigenase-2 antagonist (NS-398) reversed the threshold of von Frey test on the foot up to the control level at 2 weeks after injury. Collectively, we analyzed a new pain model showing foot hyperalgesia after thoracic burn injury and demonstrated that neurotransmission of glutamate was enhanced at the lumbar spinal cord level by immunocytochemistry and intrathecal administration of NMDA and non NMDA antagonists. Although the precise mechanism of how remote hyperalgesia at the healing stage developed in this model remains to be confirmed, substances such as trophic factors released from the regenerating skin may cause systemic hyperalgesia including in the foot.

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Cell Size and Succinate Dehydrogenase Activity of Different Types of Fibers in Different Regions of the Tibialis Anterior Muscle in Mice and Rats.

Hirofuji

Nakatani

Ishihara

et al. 2000

Acta Histochem. Cytochem

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Astroglial Elements in the Suprachiasmatic Nucleus of the Golden Hamster and the Rat. Comparative Morphological Study Using Immunocytochemistry.

Cited by 3 publications

References 29 publications

Growth-Related Changes in Skeletal Muscle Fiber Type and Insulin Resistance in Diabetic Otsuka Long-Evans Tokushima Fatty Rats.

Growth-Related Changes in Skeletal Muscle Fiber Type and Insulin Resistance in Diabetic Otsuka Long-Evans Tokushima Fatty Rats.

Foot Hyperalgesia after Thoracic Burn Injury. Histochemical, Behavioral and Pharmacological Studies.

Cell Size and Succinate Dehydrogenase Activity of Different Types of Fibers in Different Regions of the Tibialis Anterior Muscle in Mice and Rats.

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