Astroglial cell alteration caused by neurotoxins: Immunohistochemical observations with antibodies to glial fibrillary acidic protein, laminin, and tyrosine hydroxylase

Differential response of three astrocyte-specific proteins to fimbrial transection of the rat brain: Immunohistochemical observations with antibodies against glial fibrillary acidic protein, glutamine synthetase and S-100 protein.

“…Cur previous study indicates that degeneration of cell bodies in the rat striatum induced by kainic acid is followed by intensive reaction of astrocytes and that fiber degeneration without loss of cell bodies results in only a slight increase of reactive astrocytes (27). In the present study we could not discriminate between these two aspects of degeneration, because the septohippocampal pathway contains dual projections.…”

“…Serial sections which contain the septum area or the hippocampal formation were stained in turn with either one of three primary antibodies or cresyl violet. Immunostaining was carried out using avidin-biotin-peroxidase system (Vectorstain ABC kit; Vector, USA) as reported previously (27). Antibodies against GFAP and 5-100 were purchased from DAKO (Denmark) and were diluted 2,000 and 5,000 times, respectively.…”

“…This phenomenon has been reported in many studies using various procedures such as stab wounding (20), ischemic injury (21), acute surgical deafferentation of the hippocampus (11), neurotoxin injection to the striatum (27) or necrotic lesion by laser irradiation (32). The role of astrocytes in the restoration and regeneration of the CNS is not yet fully understood, while a few studies have demonstrated that astrocytes may guide immature migrating neurons and induce their neuritic growth (15,23,35).…”

Differential activation of microglia and astrocytes in aniso‐ and isomorphic gliotic tissue

“…The lack of astrocytes at the site of neuronal death induced by neurotoxin has been observed before Ogawa et al, 1989). Such total lack of GFGP-positive cells at the lesion site is quite striking.…”

Influência da adrenalectomia bilateral nos eventos neurodegenerativos no modelo do parkinsonismo experimental pela 6-OHDA nigral. Enfoque aos mecanismos parácrinos gliais envolvidos na neuroproteção e cicatrização

“…Já num quadro de lesão pela 6-OHDA, através do método imunohistoquímico verificou-se que a laminina é imunorreativa somente na área da lesão, mas não está associada ao processo de regeneração das fibras dopaminérgicas (Ogawa et al, 1989). É possível que alterações da laminina na região do corpo estriado esteja relacionada com processo de neovascularização de reparo durante o processo de cicatrização.…”