Astrocyte morphology, heterogeneity, and density in the developing African giant rat (Cricetomys gambianus)

Olude, M. A.; Mustapha, O. A.; Aderounmu, Oluwatunde A.; Olopade, James Olukayode; Ihunwo, Amadi O.

doi:10.3389/fnana.2015.00067

Cited by 35 publications

(29 citation statements)

References 45 publications

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“…from juvenile to adult, the glial and neuronal expression reduces. The latter observation agrees with the findings of Olude, Mustapha, Aderounmu et al (2015) where glial and neuronal populations were noted to decrease with age in the AGR brain. There was immuno-localization of GFAPpositive cells in the web loose connective tissue of the testis around blood vessels.…”

Section: Discussionsupporting

confidence: 92%

“…There was immuno-localization of GFAPpositive cells in the web loose connective tissue of the testis around blood vessels. These GFAP-positive cells may be perivascular astrocytes similar to those described in the AGR brain (OLUDE, MUSTAPHA, ADEROUNMU et al, 2015). Astrocytes have been known to perform many functions in the central nervous system, among which are the astrocyte end-feet encircling the endothelial cells that form the bloodbrain barrier (SOFRONIEW and VINTERS, 2010).…”

Section: Discussionmentioning

confidence: 75%

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Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus)

et al. 2017

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Introduction: This study was carried out to investigate the expression of connective tissue (Collagens I and III), glia and neuronal markers in the testis of the African giant rat using histology and immunohistochemistry techniques. Materials and Methods: Eight (8) apparently healthy wild male African giant rats were used for this experiment, divided into 2 groups (juvenile and adult) of 4 animals each. The testes were harvested following intracardial perfusion of the rats and histology was performed using Haematoxylin-Eosin stain and Mallory-Heideinhain rapid one-step staining for connective tissue. Immunohistochemical identification was achieved using the following antibodies: anti-collagen type I, anti-collagen type III, anti-glial fibrillary acidic protein and anti-p75 nerve growth factor for the expression of collagen type I, collagen type III, astrocyte-like cell and neuronal cells respectively. Photomicrography was achieved using Axioskop  microscope and quantitative data were analyzed using student t-test. Results: The cyto-architecture of the testis was typical in the African giant rat. The connective tissue expressed in the juvenile and adult group, signaling of glial-like cells were seen in the perivascular region across the experimental groups. Immuno-localization of neuronal cells were seen in the interstitial spaces across all the groups, but with more expressions in the juvenile. Conclusion: This work has provided a clear description of the expression of connective tissue, neuronal and glial cells in the testis of the African giant rat and their possible relationships across juvenile and adult groups.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 75%

Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus)

et al. 2017

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“…This technique reveals fine structures of neurons, glia and their processes, allowing a better understanding of cellular morphology along with their orientation and location in cortical slices (Melendez-Ferro et al, 2009). Tissue was fixed in 10% formalin for 2 days followed by fixation in 3% K 2 Cr 2 O 7 for 1 day and 2% AgNO 3 for another 2 days (Olude et al, 2015). The telencephalon was separated from the rest of the brain and sliced into 100 μm-thick axial slices using a Leica VT1200S vibratome (Leica, Richmond Hill, ON, Canada).…”

Section: Golgi Stainingmentioning

confidence: 99%

Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition

Hossein-Javaheri¹,

Wilkie²,

Lado

et al. 2016

Journal of Experimental Biology

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With oxygen deprivation, the mammalian brain undergoes hyperactivity and neuronal death while this does not occur in the anoxiatolerant goldfish (Carassius auratus). Anoxic survival of the goldfish may rely on neuromodulatory mechanisms to suppress neuronal hyper-excitability. As γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain, we decided to investigate its potential role in suppressing the electrical activity of goldfish telencephalic neurons. Utilizing whole-cell patch-clamp recording, we recorded the electrical activities of both excitatory ( pyramidal) and inhibitory (stellate) neurons. With anoxia, membrane potential (V m ) depolarized in both cell types from −72.2 mV to −57.7 mV and from −64.5 mV to −46.8 mV in pyramidal and stellate neurons, respectively. While pyramidal cells remained mostly quiescent, action potential frequency (AP f ) of the stellate neurons increased 68-fold. Furthermore, the GABA A receptor reversal potential (E GABA ) was determined using the gramicidin perforated-patch-clamp method and found to be depolarizing in pyramidal (−53.8 mV) and stellate neurons (−42.1 mV). Although GABA was depolarizing, pyramidal neurons remained quiescent as E GABA was below the action potential threshold (−36 mV pyramidal and −38 mV stellate neurons). Inhibition of GABA A receptors with gabazine reversed the anoxiamediated response. While GABA B receptor inhibition alone did not affect the anoxic response, co-antagonism of GABA A and GABA B receptors (gabazine and CGP-55848) led to the generation of seizure-like activities in both neuron types. We conclude that with anoxia, V m depolarizes towards E GABA which increases AP f in stellate neurons and decreases AP f in pyramidal neurons, and that GABA plays an important role in the anoxia tolerance of goldfish brain.

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“…Anatomical studies by Nzalak et al (2008), Ibe et al (2014) and Olude et al (2015) have confered a well developed olfactory system in the African giant pouched rat. This high acuity of odour perception has been attributed to the use of the rodent in the diagnosis of salmonelosis and detection of tobacco products (Mahoney et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Structure of the Main Olfactory Bulb and Immunolocalisation of Brain-Derived Neurotrophic Factor in the Olfactory Layers of the African Grasscutter (Thryonomys swinderianus - Temminck, 1827)

Ibe¹,

Ikpegbu²,

Nlebedum³

2018

AJVS

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Key words:African grasscutter, Brain derived neurotrophic factor, Main olfactory bulb, Mitral cells, olfactory glomerulusThe present study has provided some novel findings on the structure of the olfactory bulb in the African grasscutter, by basic neuro-anatomical technique and immunhistochemical technique, using antibody specific to brain-derived neurotrophic factor. Brain samples extracted from neonates on postnatal day 3, juveniles on postnatal day 72 and adults on postnatal day 450 were utilised for the study. There was a consistent increase in the relative weight of the olfactory bulb with advancement in age, but a significant (P < 0.05) decrease in the relative length from 20.81 ± 0.37 on postnatal day 3 to 17.75 ± 0.45 on postnatal day 72. The concentric lamina organization of the olfactory bulb was evident on postnatal day 3, but the glomerular layer was completely absent. The layer was evident on postnatal days 72 and 450. The mean diameters of each glomerulus on postnatal days 72 and 450 were 86.82 ± 0.25 µm and 117.14 ± 0.32 µm, respectively. The difference was significant (P < 0.05). The zones of the external plexiform layer were undifferentiated on postnatal day 3. There was a decrease in the number of mitral cell layer, but increase in size of each mitral cell, with advancement in age. Brain-derived neurotrophic factor was moderately immunolocalised in the olfactory nerve layer on postnatal day 3, but negative on postnatal days 72 and 450. Cell bodies of the periglomerular short-axon neurones mildly expressed brain-derived neurotrophic factor on postnatal days 72 and 450. The granule cells did not express brain-derived neurotrophic factor on all postnatal periods. The functional significance of the results was discussed.

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Astrocyte morphology, heterogeneity, and density in the developing African giant rat (Cricetomys gambianus)

Cited by 35 publications

References 45 publications

Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus)

Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus)

Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition

Structure of the Main Olfactory Bulb and Immunolocalisation of Brain-Derived Neurotrophic Factor in the Olfactory Layers of the African Grasscutter (Thryonomys swinderianus - Temminck, 1827)

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