Astrocyte Ca2+ signalling: an unexpected complexity

Volterra, Andrea; Liaudet, Nicolas; Savtchouk, Iaroslav

doi:10.1038/nrn3725

Cited by 382 publications

(388 citation statements)

References 112 publications

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“…This does not necessarily imply that elegant experimental manipulations with astroglial Ca 2+ within a certain dynamic range by triggering certain cellular cascades should reproduce such effects (Agulhon et al, 2010; Fiacco et al, 2007; Petravicz et al, 2008) (see (Rusakov et al, 2014; Volterra et al, 2014) for discussion). In addition to the much debated astrocyte‐neuron exchange, Ca 2+ rises in astrocytes could also boost the expression level of glutamate transporters (Devaraju et al, 2013), re‐position mitochondria closer to glutamate transporters (Jackson et al, 2014; Ugbode et al, 2014), and regulate neuro‐metabolic coupling with neurons (Bernardinelli et al, 2004; Porras et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…5A). It appears that STED imaging could also reveal the heterogeneous distribution of purinergic P2Y1 receptors along astrocyte processes (Volterra et al, 2014) (Fig. 5B).…”

Section: Monitoring Of Astroglia On the Nanoscale: Emerging Techniquesmentioning

confidence: 94%

“…However, PAPs do seem to contain individual ribosomes and glycogen granules, together with actin and actin binding proteins (Bernardinelli et al, 2014a; Derouiche and Frotscher, 2001; Fiala et al, 2003; Molotkov et al, 2013; Safavi‐Abbasi et al, 2001; Seidel et al, 1995). Moreover, recent results illustrate that despite general believe, fine astrocyte processes do contain some types of calcium stores and sinks as seen with EM (Lovatt et al, 2007; Sahlender et al, 2014; Volterra et al, 2014). …”

Section: Molecular Makeup Of Perisynaptic Astroglial Processes: Majormentioning

confidence: 94%

“…Across astrocyte types, mGluR signaling through Gq proteins triggers calcium release from intracellular stores such as the endoplasmic reticulum (Volterra et al, 2014). This cascade in turn might influence actin dynamics and hence filopodia growth, as suggested in studies using calcium uncaging in astrocyte cultures and genetic attenuation of IP 3 signaling in vivo (Molotkov et al, 2013; Tanaka et al, 2013).…”

Section: Triggering Structural Plasticity Of Astroglia By Excitatory mentioning

confidence: 99%

“…( B ) STED image of P2Y1 receptors (red) along a multi‐branched astrocytic process (glutamine synthase, grey) in the adult mouse hippocampus. Adapted from (Volterra et al, 2014). ( C ) STORM imaging of pre‐ (Bassoon; red) and postsynaptic (Homer1; green) scaffolding proteins in the mouse main olfactory bulb glomeruli imaged using conventional fluorescence imaging (C1) and STORM (C2); adapted from (Dani et al, 2010).…”

Section: Monitoring Of Astroglia On the Nanoscale: Emerging Techniquesmentioning

confidence: 99%

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Morphological plasticity of astroglia: Understanding synaptic microenvironment

Heller

Rusakov

2015

Glia

132

137

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Memory formation in the brain is thought to rely on the remodeling of synaptic connections which eventually results in neural network rewiring. This remodeling is likely to involve ultrathin astroglial protrusions which often occur in the immediate vicinity of excitatory synapses. The phenomenology, cellular mechanisms, and causal relationships of such astroglial restructuring remain, however, poorly understood. This is in large part because monitoring and probing of the underpinning molecular machinery on the scale of nanoscopic astroglial compartments remains a challenge. Here we briefly summarize the current knowledge regarding the cellular organisation of astroglia in the synaptic microenvironment and discuss molecular mechanisms potentially involved in use‐dependent astroglial morphogenesis. We also discuss recent observations concerning morphological astroglial plasticity, the respective monitoring methods, and some of the newly emerging techniques that might help with conceptual advances in the area. GLIA 2015;63:2133–2151

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Section: Discussionmentioning

confidence: 99%

“…5A). It appears that STED imaging could also reveal the heterogeneous distribution of purinergic P2Y1 receptors along astrocyte processes (Volterra et al, 2014) (Fig. 5B).…”

Section: Monitoring Of Astroglia On the Nanoscale: Emerging Techniquesmentioning

confidence: 94%

Section: Molecular Makeup Of Perisynaptic Astroglial Processes: Majormentioning

confidence: 94%

Section: Triggering Structural Plasticity Of Astroglia By Excitatory mentioning

confidence: 99%

Section: Monitoring Of Astroglia On the Nanoscale: Emerging Techniquesmentioning

confidence: 99%

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Morphological plasticity of astroglia: Understanding synaptic microenvironment

Heller

Rusakov

2015

Glia

132

137

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Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression

et al. 2020

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The ATP1A2 coding α2 subunit of Na,K‐ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2tmCKwk/+ crossed with transgenic mice expressing G‐CaMP7 in cortical neurons and astrocytes (Atp1a2+/−), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− were higher than those in wild‐type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− may contribute to FHM2 pathophysiology.

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Electrical Stimulation by an Organic Transistor Architecture Induces Calcium Signaling in Nonexcitable Brain Cells

Borrachero-Conejo

Saracino

Natali

et al. 2018

Adv Healthcare Materials

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materialsdisplay intrinsic multi functionality, combining ionoelectronic transport properties, photonic properties, and optical transparency within the visible range with improved biocompatibility with neural cells compared to inorganic sub strates. [1][2][3][4] Notably, the survival of primary neuronal cells on organic bioelectronic polymers [1,3,4] and small molecules [5,6] is higher when compared to silicon/glass substrates, while the functionality of brain cells is preserved on organic biofunctional interfaces. [1,5] Importantly, organic bioelec tronic [3,7] and biooptoelectronic [8,9] devices capable to record, stimulate, and modulate functionality of neuronal cells in vitro [7] and in vivo [3] have been reported, showing the potential and the relevance for clinical neurology as well as for neuroscience fun damental studies.Recently, the role of glial cells, called astrocytes, is emerging among the chal lenges and targets that need to be con sidered for the development of devices devoted to neuroscience investigations and applications. Astrocytes are, indeed, the cells that are majorly involved in the regulation of the concentration of ions and neu rotransmitters in the synaptic cleft, participating to communica tion signals between neurons and playing a pivotal role in the brain physio logy. [10,11] The function of astrocytes mainly depends on the activity of transmembrane proteins forming transporters, Organic bioelectronics have a huge potential to generate interfaces and devices for the study of brain functions and for the therapy of brain pathologies. In this context, increasing efforts are needed to develop technologies for monitoring and stimulation of nonexcitable brain cells, called astrocytes. Astroglial calcium signaling plays, indeed, a pivotal role in the physiology and pathophysiology of the brain. Here, the use of transparent organic cell stimulating and sensing transistor (O-CST) architecture, fabricated with N,N′-ditridecylperylene-3,4,9,10-tetracarboxylic diimide (P13), to elicit and monitor intracellular calcium concentration ([Ca 2+ ] i ) in primary rat neocortical astrocytes is demonstrated. The transparency of O-CST allows performing calcium imaging experiments, showing that extracellular electrical stimulation of astrocytes induces a drastic increase in [Ca 2+ ] i . Pharmacological studies indicate that transient receptor potential (TRP) superfamily are critical mediators of the [Ca 2+ ] i increase. Experimental and computational analyses show that [Ca 2+ ] i response is enabled by the O-CST device architecture. Noteworthy, the extracellular field application induces a slight but significant increase in the cell volume. Collectively, it is shown that the O-CST is capable of selectively evoking astrocytes [Ca 2+ ] i , paving the way to the development of organic bioelectronic devices as glial interfaces to excite and control physiology of non-neuronal brain cells.

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Astrocyte Ca2+ signalling: an unexpected complexity

Cited by 382 publications

References 112 publications

Morphological plasticity of astroglia: Understanding synaptic microenvironment

Morphological plasticity of astroglia: Understanding synaptic microenvironment

Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression

Electrical Stimulation by an Organic Transistor Architecture Induces Calcium Signaling in Nonexcitable Brain Cells

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