Astragalus polysaccharides protect cardiac stem and progenitor cells by the inhibition of oxidative stress-mediated apoptosis in diabetic hearts

Abstract

IntroductionDiabetic cardiomyopathy is characterized by an imbalance between myocyte death and regeneration mediated by the progressive loss of cardiac stem and progenitor cells (CSPCs) by apoptosis and necrosis due to the activation of oxidative stress with diabetes. In this study, we evaluated the beneficial effect of astragalus polysaccharides (APS) therapy on the protection of CSPCs through its antioxidative capacity in diabetic hearts.Materials and methodsStreptozotocin (STZ)-induced diabetic mice and heterozygous (SOD2+/−) knockout mice were employed and administered with APS. Ventricular CSPCs were isolated for oxidative evaluation. The abundance, apoptosis and proliferation, reactive oxygen species (ROS) formation, oxidative damage, and SOD2 protein levels and activities were evaluated in ventricular CSPCs.ResultsWe confirmed that APS increased the CSPC abundance, reduced the apoptosis of CSPCs, and enhanced the proliferation of CSPCs in both STZ-induced diabetic mice and nondiabetic SOD2+/− mice. In addition, therapy of APS enhanced SOD2 protein levels and enzyme activities, and inhibited ROS formation and oxidative damage of CSPCs from both STZ-induced diabetic mice and nondiabetic SOD2+/− mice.ConclusionOur findings demonstrated the positive effect of APS on the rescue of CSPC preservation in diabetes, dependent on the inhibition of oxidative stress-mediated apoptosis.

Astragalus Polysaccharide Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Oxidative Damage and Mitochondrial Dysfunction

“…Several studies have reported that ROSspecific inhibitor effectively alleviated cisplatin-induced kidney toxicity by reducing ROS accumulation [35]. APS has been reported to be a natural antioxidant in various pathological states [14,18,36,37]. As anticipated, in our study, APS also could mitigate cisplatin-induced oxidative damage by reducing ROS generation and recovering the activities of T-SOD and GSH-Px.…”

“…Astragalus polysaccharide (APS) is composed of glucans and heteropolysaccharide. Previous studies have indicated that APS might possess anti-inflammatory, antioxidant, and mitochondria protection properties, which could ameliorate inflammatory response, oxidative injury, and mitochondrial dysfunction in many different pathological conditions [14][15][16][17][18]. Furthermore, studies have suggested that APS may attenuate mitochondrial injury caused by ROS, possessing antiaging activity [19].…”

RETRACTED ARTICLE: Astragalus polysaccharide alleviates H 2 O 2 -triggered oxidative injury in human umbilical vein endothelial cells via promoting KLF2

“…At present, most researches about APS focus on the protection of cardiac myocytes, and the anti-oxidant effect of APS has been reported. Chen et al testified that APS could protect cardiac stem and progenitor cells through restraining oxidative stress-regulated apoptosis in diabetic hearts [30]. Han et al also reported that APS could protect HUVECs against injury triggered by H 2 O 2 through accelerating the cell antioxidant capacity and NO bioavailability [31].…”

MMP9 mediates acute hyperglycemia-induced human cardiac stem cell death by upregulating apoptosis and pyroptosis in vitro

“…Hyperglycemia upregulates oxidative stress by increasing the production of reactive oxygen species (ROS) 21,22 . To investigate if MMP9 mediates hyperglycemia-induced ROS production in hCSCs, we treated hCSCs and MMP9 −/− hCSCs with NG and HG for 24 h, and measured cellular levels of ROS by CellROX staining.…”