Astragalus polysaccharide inhibits isoprenaline-induced cardiac hypertrophy via suppressing Ca2+-mediated calcineurin/NFATc3 and CaMKII signaling cascades

Dai, Huanyun; Jia, Guizhi; Liu, Xin; Liu, Zhining; Wang, Hongxin

doi:10.1016/j.etap.2014.05.008

Cited by 39 publications

(30 citation statements)

References 41 publications

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“…The criteria for selection of drug concentrations of Iso (10 M) and APS (100 g/ml) were based on our previous report which showed that treatment of NRVMs with 10 M Iso for 48 h caused significant hypertrophy, which were effectively inhibited by APS (50-200 g/ml) in a dose dependent manner. Therefore, we selected the concentrations of 10 M for Iso and 100 g/ml for APS respectively in the present study (Dai et al, 2014). APS was dissolved in dimethylsulfoxide (DMSO) and the final DMSO concentration did not exceed 0.1% (v/v).…”

Section: Cell Experimentsmentioning

confidence: 99%

“…The experiments with NRVMs were performed after 24-48 h culture when synchronously contracting cells were observed (Dai et al, 2014). Briefly, the media was changed to serumfree Dulbecco's modified Eagle's medium (DMEM, Gibco Chemical Co, Shanghai, China) supplemented with 1% penicillin-streptomycin (Invitrogen, Shanghai, China) 24 h before APS treatments.…”

Section: Cell Experimentsmentioning

confidence: 99%

“…Neonatal rat ventricular myocytes (NRVMs) were isolated and cultured as described previously (Dai et al, 2014). The experiments with NRVMs were performed after 24-48 h culture when synchronously contracting cells were observed (Dai et al, 2014).…”

Section: Cell Experimentsmentioning

confidence: 99%

“…Study revealed that APS alleviated diabetic cardiomyopathy with normalization of decreased cardiac function (Chen et al, 2010), indicating that APS might possess potential cardioprotective effects. We recently reported that APS attenuates hypertrophy of neonatal rat ventricular myocytes (NRVMs) induced by Iso (Dai et al, 2014). Whether and how APS alleviates cardiac hypertrophy in animals treated by Iso remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Astragalus polysaccharide attenuates isoproterenol-induced cardiac hypertrophy by regulating TNF-α/PGC-1α signaling mediated energy biosynthesis

Luan¹,

Tang²,

Yang³

et al. 2015

Environmental Toxicology and Pharmacology

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Section: Cell Experimentsmentioning

confidence: 99%

Section: Cell Experimentsmentioning

confidence: 99%

Section: Cell Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Astragalus polysaccharide attenuates isoproterenol-induced cardiac hypertrophy by regulating TNF-α/PGC-1α signaling mediated energy biosynthesis

Luan¹,

Tang²,

Yang³

et al. 2015

Environmental Toxicology and Pharmacology

Self Cite

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“…However, the molecular events, which underlie the aberrant expression of NFATC3 in cardiomyocyte hypertrophy remain to be fully elucidated, although its pro-hypertrophic function has been well-established in previous studies (18,19). In addition, the present study provided further clarification of the molecular signaling pathway responsible for the high expression levels of NFATC3 during the hypertrophic process.…”

Section: Discussionmentioning

confidence: 58%

MicroRNA-1 suppresses cardiac hypertrophy by targeting nuclear factor of activated T cells cytoplasmic 3

Yin

Zhao

Zhang

et al. 2012

Molecular Medicine Reports

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Abstract. Cardiomyocyte hypertrophy is a threat to human health due to the probability of sudden heart failure-induced mortality. Previous studies have demonstrated that nuclear factor of activated T cells cytoplasmic 3 (NFATC3) is important in the process of cardiomyocyte hypertrophy. However, the molecular mechanism underlying the alteration in the expression levels of NFATC3 during cardiomyocyte hypertrophy has remained to be fully elucidated. In order to shed light on the molecular mechanism, the present study employed several approaches, including the measurement of the cell surface area, analysis of the protein/DNA ratio, western blot analysis and a Luciferase reporter assay using isolated rat cardiomyocytes as model. The results showed that expression of microRNA-1 (miR-1) was reduced in patients diagnosed with cardiac hypertrophy and rat cardiomyocytes treated with pro-hypertrophic stimuli. The increase in the expression of miR-1 was able to inhibit the hypertrophic remodeling of cardiomyocytes. The suppression of miR-1 was sufficient to induce cardiomyocyte hypertrophy, and further experiments confirmed that NFATC3 was a target of miR-1 in cardiomyocytes. Forced expression of NFATC3 inhibited the protective activity of miR-1 against hypertrophic stimuli in the cardiomyocytes. These findings provided clarification of the regulatory signaling pathway underlying cardiac hypertrophy, and provided evidence that targeting the miR-1/NFATC3 pathway may be a promising strategy for the prevention and treatment of heart hypertrophy.

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Phytochemistry and cardiovascular protective effects of Huang‐Qi (Astragali Radix)

Shaker

Kuang

et al. 2021

Medicinal Research Reviews

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Huang‐Qi (Astragali Radix) is an herbal tonic widely used in China and many other countries. It is derived from the roots of Astragalus membranaceus and A. membranaceus var. mongholicus and shows potent cardiovascular protective effects. In this article, we comprehensively reviewed 189 small molecules isolated from the two Astragalus species and discussed the interspecies chemical differences. Moreover, we summarized the pharmacological activities and mechanisms of action of Huang‐Qi and its major bioactive compounds for the treatment of cardiovascular diseases. This review covers 171 references published between February 1983 and March 2020.

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Astragalus polysaccharide inhibits isoprenaline-induced cardiac hypertrophy via suppressing Ca2+-mediated calcineurin/NFATc3 and CaMKII signaling cascades

Cited by 39 publications

References 41 publications

Astragalus polysaccharide attenuates isoproterenol-induced cardiac hypertrophy by regulating TNF-α/PGC-1α signaling mediated energy biosynthesis

Astragalus polysaccharide attenuates isoproterenol-induced cardiac hypertrophy by regulating TNF-α/PGC-1α signaling mediated energy biosynthesis

MicroRNA-1 suppresses cardiac hypertrophy by targeting nuclear factor of activated T cells cytoplasmic 3

Phytochemistry and cardiovascular protective effects of Huang‐Qi (Astragali Radix)

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