Astilbin from Smilax glabra Roxb. Attenuates Inflammatory Responses in Complete Freund’s Adjuvant‐Induced Arthritis Rats

Abstract

Astilbin, a flavonoid compound, was isolated from the rhizome of Smilax glabra Roxb. (with red cross-section) grown in Guizhou Province, China. We accessed its effect and potential mechanism on attenuation of the inflammatory response in CFA-induced AA rats. Our results showed that daily oral administration of astilbin at 5.3 mg/kg reduced joint damage in the hind paw of AA rats. Accordingly, astilbin exhibited remarkable inhibitory effects on TNF-α, IL-1β, and IL-6 mRNA expression. Significant decrease of serum cytokine levels of TNF-α, IL-1β, and IL-6 was also observed in astilbin-treated AA rats compared to the vehicle-treated AA rats. The reduced expression of these cytokines was associated with protein activity suppression of three key molecular targets in the pathogenesis of RA, including IKKβ, NF-κB p65 subunit, and TLR adaptor MyD88. Furthermore, the therapeutic effects of astilbin on the inhibition of cytokines production as well as the reduction of inflammatory response in AA rats are close to a commonly used antirheumatic drug, leflunomide. Collectively, our data suggest that the action mechanism of astilbin, as an anti-inflammatory agent for RA treatment, is associated with modulating the production of proinflammatory cytokines and inhibiting the expression of key elements in NF-κB signaling pathway mediated by TLR.

Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview

“…Their results showed noteworthy inhibitory properties of astilbin on TNF-α, IL-1β as well as IL-6 mRNA expression; serum cytokine levels of TNF-α, IL-1β, and IL-6 were also decreased in treated AA rats. They also proved that oral treatment of astilbin daily at 5.3 mg/kg can reduce joint damage in hind paw of the animal model; this therapeutic properties of astilbin flavonoid on the inhibition of cytokines production and reduction of inflammatory response in in vivo AA rats model were effective as equal as leflunomide, the frequently used antirheumatic drug [ 74 ]. Moreover, the double-blind, randomized, placebo-controlled clinical trial on inflammation of ferulic acid, an abundant phenolic compound from various plant including edible medicinal plant and cereal grains, were evaluated in hyperlipidemic subjects by the research team of Bumrungpert.…”

“…These processes are an indispensable step to promote the progression in drug discovery and development using phytochemical compounds. Asian region is well-known as one of the greatest hotspot of plant biodiversity including medicinal species, especially Japan, China, Thailand and related areas in tropical and sub-tropical regions [ 6 , 54 , 67 , 74 , 104 , 105 ]. However, a comprehensive profiling of many medicinal plants and functional evaluation of their chemical compound has not been completely conducted.…”

Corrigendum to “Astilbin from Smilax glabra Roxb. Attenuates Inflammatory Responses in Complete Freund’s Adjuvant‐Induced Arthritis Rats”

“…In the article titled “Astilbin from Smilax glabra Roxb. Attenuates Inflammatory Responses in Complete Freund's Adjuvant-Induced Arthritis Rats” [1], the affiliation of Dr. Jinqiu Zhu should be “Department of Epidemiology and Environmental Health, The State University of New York, Buffalo, NY, USA” only. The updated authors' list and affiliations are shown above.…”

Molecular Insight into the Binding of Astilbin with Human Serum Albumin and Its Effect on Antioxidant Characteristics of Astilbin

“…Studies indicate that flavonols could reduce the risk of chronic illnesses, and some have antioxidant, anti-inflammatory, anti-diabetic or neuroprotective properties [ 3 ]. Astilbin ( Supplementary Materials, Figure S1 ) is a dihydroflavonol rhamnoside found in numerous natural plants, such as Hypericum perforatum [ 4 ] and Smilax glabra Roxb [ 5 ]. Meanwhile, astilbin is prevalent in blades and veins of healthy grapevine leaves [ 6 ].…”