Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells

Zhao, Yi‐Fang; Zhu, Changliang; Li, Xiaofei; Zhang, Zhipei; Yuan, Yuan; Ni, Yunfeng; Liu, Tonggang; Deng, Sanming; Zhao, Jinbo

doi:10.3892/or.2011.1358

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…Its molecular mechanism of anti-proliferative effects was related to the regulation of the expression of anti- and pro-apoptosis proteins (Bcl-2, and Bax) and DNA fragmentation [ 26 ]. Additionally, asterosaponin 1 suppressed the proliferation of human lung cancer A549 cells via ER-apoptosis [ 8 ]. Later, the same group of authors found that novaeguinoside II (asterosaponin from the starfish Culcita novaeguineae ) was able to induce apoptosis in U87MG cells by increasing cytochrome C and caspase-3 protein expression levels [ 7 ].…”

Section: Resultsmentioning

confidence: 99%

“…For example, novaeguinoside II and asterosaponin 1 from the starfish Culcita novaeguineae have cytostatic and pro-apoptotic activities against human glioblastoma U87MG cells [ 7 ]. Furthermore, asterosaponin 1 was shown to induce apoptosis through the regulation of endoplasmic reticulum (ER-apoptosis) and, consequently, decreased the proliferation rate of human lung cancer A549 cells [ 8 ]. The anti-proliferative effect of polyhydroxysteroid glycosides from the starfish Anthenea chinensis has been associated with the prevention of tubulin polymerization-promotion in human glioblastoma cells [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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The Inhibitory Activity of Luzonicosides from the Starfish Echinaster luzonicus against Human Melanoma Cells

et al. 2017

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Malignant melanoma is the most dangerous form of skin cancer, with a rapidly increasing incidence rate. Despite recent advances in melanoma research following the approval of several novel targeted and immuno-therapies, the majority of oncological patients will ultimately perish from the disease. Thus, new effective drugs are still required. Starfish steroid glycosides possess different biological activities, including antitumor activity. The current study focused on the determination of the in vitro inhibitory activity and the mechanism of action of cyclic steroid glycosides isolated from the starfish Echinaster luzonicus—luzonicoside A (LuzA) and luzonicoside D (LuzD)—in human melanoma RPMI-7951 and SK-Mel-28 cell lines. LuzA inhibited proliferation, the formation of colonies, and the migration of SK-Mel-28 cells significantly more than LuzD. Anti-cancer activity has been ascribed to cell cycle regulation and apoptosis induction. The molecular mechanism of action appears to be related to the regulation of the activity of cleaved caspase-3 and poly(ADP-ribose) polymerase (PARP), along with Survivin, Bcl-2, p21 and cyclin D1 level. Overall, our findings support a potential anti-cancer efficacy of luzonicosides A and D on human melanoma cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Inhibitory Activity of Luzonicosides from the Starfish Echinaster luzonicus against Human Melanoma Cells

et al. 2017

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“…When external stimuli or damage lead to ER Ca 2+ depletion, GRP78 releases IRE1/PERK and subsequently activates ER stress 43 , 44 . ER stress subsequently leads to the activation of CHOP or inhibition of BCL-2 to promote cell apoptosis 45 . In our study, under OGD/R, ER stress-related proteins were activated, and the expression of BCL-2 was inhibited; however, NGR1 suppressed the activation of ER stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

Notoginsenoside R1 Alleviates Oxygen–Glucose Deprivation/Reoxygenation Injury by Suppressing Endoplasmic Reticulum Calcium Release via PLC

Wang

et al. 2017

Sci Rep

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As documented in our previous study, notoginsenoside R1 (NGR1) can inhibit neuron apoptosis and the expression of endoplasmic reticulum (ER) stress-associated pro-apoptotic proteins in hypoxic–ischemic encephalopathy. Recent evidence indicates that the Phospholipase C (PLC)/inositol 1,4,5-trisphosphate receptor (IP3R) is important for the regulation of Ca2+ release in the ER. Ca2+ imbalance can stimulate ER stress, CAMKII, and cell apoptosis. The purpose of this study was to further investigate the neuroprotective effect of NGR1 and elucidate how NGR1 regulates ER stress and cell apoptosis in the oxygen–glucose deprivation/reoxygenation (OGD/R) model. Cells were exposed to NGR1 or the PLC activator m-3M3FBS. Then, IP3R- and IP3-induced Ca2+ release (IICR) and activation of the ER stress and CaMKII signal pathway were measured. The results showed that NGR1 inhibited IICR and strengthened the binding of GRP78 with PERK and IRE1. NGR1 also alleviated the activation of the CaMKII pathway. Pretreatment with m-3M3FBS attenuated the neuroprotective effect of NGR1; IICR was activated, activation of the ER stress and CaMKII pathway was increased, and more cells were injured. These results indicate that NGR1 may suppress activation of the PLC/IP3R pathway, subsequently inhibiting ER Ca2+ release, ER stress, and CaMKII and resulting in suppressed cell apoptosis.

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“…For example, effective cytostatics, asterosaponin 1 and novaeguinoside II from the starfish Culcita novaeguineae induced apoptosis of human glioblastoma U87MG cells through several signaling transduction pathways [ 6 , 7 ]. Moreover, asterosaponin 1 inhibited the proliferation of A549 human lung cancer cells through induction of endoplasmic reticulum stress-associated apoptosis [ 8 ]. Polyhydroxysteroid glycosides isolated from the starfish Anthenea chinensis exhibited significant activity against promotion of tubulin polymerization in vitro inhibiting the proliferation of glioblastoma cells [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Four New Sulfated Polar Steroids from the Far Eastern Starfish Leptasterias ochotensis: Structures and Activities

Malyarenko¹,

Malyarenko²,

Ivanchina³

et al. 2015

Marine Drugs

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Three new sulfated steroid monoglycosides, leptaochotensosides A–C (1–3), and a new sulfated polyhydroxylated steroid (4) were isolated from the alcoholic extract of the Far Eastern starfish Leptasterias ochotensis. The structures of compounds 1–4 were established by extensive nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESIMS) analyses and chemical transformations. Although the isolated compounds did not show any apparent cytotoxicity against melanoma RPMI-7951 and breast cancer T-47D cell lines, leptaochotensoside A (1) demonstrated inhibition of T-47D cell colony formation in a soft agar clonogenic assay at nontoxic doses. In addition, this compound decreased the epidermal growth factor (EGF)-induced colony formation of mouse epidermal JB6 Cl41 cells. The cancer preventive action of 1 is realized through regulation of mitogen-activated protein kinase (MAPK) signaling pathway.

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Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells

Cited by 10 publications

References 33 publications

The Inhibitory Activity of Luzonicosides from the Starfish Echinaster luzonicus against Human Melanoma Cells

The Inhibitory Activity of Luzonicosides from the Starfish Echinaster luzonicus against Human Melanoma Cells

Notoginsenoside R1 Alleviates Oxygen–Glucose Deprivation/Reoxygenation Injury by Suppressing Endoplasmic Reticulum Calcium Release via PLC

Four New Sulfated Polar Steroids from the Far Eastern Starfish Leptasterias ochotensis: Structures and Activities

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