Astaxanthin counteracts the effects of heat shock on the maturation of bovine oocytes

Abstract

The cellular mechanisms induced by elevated temperature on oocytes are not fully understood. However, there is evidence that some of the deleterious effects of heat shock are mediated by a heat-induced increase in reactive oxygen species (ROS). In this context, carotenoid antioxidants might have a thermoprotective effect. Therefore, the objective of this study was to determine the role of astaxanthin (AST) on oocyte ROS production and on the redox profile and developmental competency of cumulus-oocyte complexes (COCs) after 14h heat shock (41°C) during in vitro maturation (IVM). Exposure of oocytes to heat shock during IVM increased ROS and reduced the ability of the oocyte to cleave and develop to the blastocyst stage. However, 12.5 and 25nM astaxanthin rescued these negative effects of heat shock; astaxanthin counteracted the heat shock-induced increase in ROS and restored oocyte developmental competency. There was no effect of astaxanthin on maturation medium lipid peroxidation or on glutathione peroxidase and catalase activity in oocytes and cumulus cells. However, astaxanthin stimulated superoxide dismutase (SOD) activity in heat-shocked cumulus cells. In conclusion, direct heat shock reduced oocyte competence, which was restored by astaxanthin, possibly through regulation of ROS and SOD activity in oocytes and COCs.

Effects of melatonin on production of reactive oxygen species and developmental competence of bovine oocytes exposed to heat shock and oxidative stress duringin vitromaturation

“…It has been repeatedly demonstrated, both in the present experiments and by others, that heat shock increases ROS production by the bovine oocyte (Nabenishi et al, 2012;Ispada et al, 2018) and reduces the percentage of oocytes that cleave after coincubation with spermatozoa (Roth & Hansen, 2004a;de Castro e Paula & Hansen, 2007). In some cases, the deleterious actions of heat shock on the oocyte also compromise the ability of the subsequent embryo to develop to the blastocyst stage (Roth et al, 2004a,b;Nabenishi et al, 2012;Rodriques et al, 2016) although this consequence of heat shock has not always observed (de Castro e Paula & Hansen, 2007;Cebrian-Serrano et al, 2013).…”

“…Heat shock may disrupt oocyte function, at least in part, by increasing the generation of reactive oxygen species (ROS). Production of ROS is increased by heat shock (Nabenishi et al , 2012; Ispada et al , 2018) and antioxidants such as retinol (Lawrence et al , 2004), cysteine (Nabenishi et al , 2012) and astaxanthin (Ispada et al , 2018) can reduce the negative consequences of heat shock on the oocyte. Moreover, the function of the maturing oocyte can be disrupted by other oxidative stresses, as shown for the nitric oxide donor sodium nitroprusside in the bovine (Soto et al , 2003; Cheuquemán et al , 2015) and hydrogen peroxide in the mouse (Tamura et al , 2008) and pig (Yazaki et al , 2013).…”

Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro

“…One of the basic factors is essential culture conditions of the oocyte during in vitro maturation (IVM) for normal maturation (Cetica et al, 2001). Maturating oocytes (Roth and Hansen 2004;Sen, 2014) in in vitro at high temperature (Lánská et al, 2006) (41 °C) were initiated cellular and molecular changes in the oocyte that causing to the decreased potential of development (Ispada et al, 2018). During maturation 12-14 h heat shock interrupted oocyte microtubule and microfilament texture (Roth and Hansen 2005) and spindle positioning (Ju et al 2005), diminished nuclear maturation (Roth and Hansen 2005;Rodrigues et al 2016), affection distribution of cortical granule (Maya-Soriano et al 2013) and reducing mitochondrial function in oocyte (Nabenishi et al 2012).…”

“…It has been studied for a long time to understand effect heat stress on mature and immature cattles oocytes and also it caused to infertility (Ispada et al, 2018). Cattle oocytes are susceptible to environmental condition factors such as heat stress, temperature and humidity…”

result of in vitro fertilazation of Mongolian sheep oocytes

“…Бидний туршилтдаа ашигласан BO-IVM, BO-IVF, BO-IVC орчинд үр тогтооход үр тогтолтын хувь бусад орчноосоо харьцангуй өндөр байгаа нь цаашид энэхүү орчинг хэрэглэх нь илүү үр дүнтэй байх боломжтойг илэрхийлж байна. Өндгөн эсийг өсгөвөрлөх температур нь (41°C), астаксантинг 12.5 болон 25 nM хэмжээтэй уусмалд хийж өндгөн эсийг өсгөвөрлөхөд дулааны цочролоос үүсэх сөрөг нөлөө бага бластоцист шатанд хүрч сайн үр дүнтэй байсан бол 50nM астаксантин агуулсан үед эерэг нөлөө үзүүлдэггүй байна [15].…”