Associative learning in larval and adult Drosophila is impaired by the dopamine-synthesis inhibitor 3-Iodo-L-tyrosine

Thoener, Juliane; König, Christian; Weiglein, Aliće; Toshima, Naoko; Mancini, Nino; Amin, Fatima; Schleyer, Michael

doi:10.1101/2020.10.26.354688

Cited by 5 publications

(19 citation statements)

References 79 publications

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“…This is likely to be the case, given related results using broader drivers, at least for reward memory (Rohwedder et al, 2016;Thoener et al, 2021) and punishment memory (Selcho et al, 2009), and it is consistent with findings in adult Drosophila (Aso et al, 2019;Handler et al, 2019) and in mammals (Navratilova et al, 2015; but see König et al, 2018;Niens et al, 2017).…”

Section: Discussionsupporting

confidence: 83%

“…• In both life stages, reinforcing effects were strongly determined by the time point of the occurrence/ termination of reinforcement, whereas its duration was only of impact for memories related to reinforcement termination (Fig. 1B-E; larvae DAN activation: Weiglein et al, 2021;adults DAN activation: König et al, 2018;electric shock: Diegelmann et al, 2013;Jacob and Waddell, 2020).…”

Section: Discussionmentioning

confidence: 97%

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Optogenetically induced reward and ‘frustration’ memory in larval Drosophila

Thoener

Weiglein

Gerber

et al. 2022

Preprint

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Humans and animals alike form oppositely valenced memories for stimuli that predict the occurrence versus the termination of a reward: appetitive 'reward' memory for stimuli associated with the occurrence of a reward and aversive 'frustration' memory for stimuli that are associated with its termination. We characterize these memories in larval Drosophila using a combination of Pavlovian conditioning, optogenetic activation of the dopaminergic central-brain DAN-i1864 neuron, and high-resolution video-tracking. This reveals their dependency on the number of training trials and the duration of DAN-i1864 activation, their temporal stability, and the parameters of locomotion that are modulated during memory expression. Together with previous results on 'punishment' versus 'relief' learning by DAN-f1 neuron activation, this reveals a 2x2 matrix of timing-dependent memory valence for the occurrence/ termination of reward/ punishment. These findings should aid the understanding and modelling of how brains decipher the predictive, causal structure of events around a target reinforcing occurrence.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 97%

Optogenetically induced reward and ‘frustration’ memory in larval Drosophila

Thoener

Weiglein

Gerber

et al. 2022

Preprint

Self Cite

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“…Procedures followed Saumweber et al (2018) andWeiglein et al (2021). Variations in the following procedures will be mentioned in the Results section.…”

Section: Odour-dan Associative Learningmentioning

confidence: 99%

“…Whether the mechanisms of frustration memory established by termination of DAN-i1 864 activation differ from those of the likewise aversive memory established by unpaired presentations of odour and DAN-i1 864 activation (Schleyer et al, 2018(Schleyer et al, , 2020 remains to be tested. The DAN-f1 neuron, in contrast, supports aversive punishment memory upon forward training and appetitive 'relief' memory upon backward training (Weiglein et al, 2021; using SS02180-Gal4 as the driver, henceforth DAN-f1 2180 ) (adults: Aso and Rubin, 2016;Handler et al, 2019;König et al, 2018). Whereas for DAN-f1 2180 Weiglein et al ( 2021) have characterized punishment and relief memories in some detail, no further analysis has been performed for the reward and frustration memories established by DAN-i1 864 activation.…”

Section: Introductionmentioning

confidence: 99%

Optogenetically induced reward and ‘frustration’ memory in larval Drosophila melanogaster

Thoener

Weiglein

Gerber

et al. 2022

Journal of Experimental Biology

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Humans and animals alike form oppositely valenced memories for stimuli that predict the occurrence versus the termination of a reward: appetitive ‘reward’ memory for stimuli associated with the occurrence of a reward and aversive ‘frustration’ memory for stimuli that are associated with its termination. We characterize these memories in larval Drosophila using a combination of Pavlovian conditioning, optogenetic activation of the dopaminergic central-brain DAN-i1864 neuron, and high-resolution video-tracking. This reveals their dependency on the number of training trials and the duration of DAN-i1864 activation, their temporal stability, and the parameters of locomotion that are modulated during memory expression. Together with previous results on ‘punishment’ versus ‘relief’ learning by DAN-f1 neuron activation, this reveals a 2x2 matrix of timing-dependent memory valence for the occurrence/ termination of reward/ punishment. These findings should aid the understanding and modelling of how brains decipher the predictive, causal structure of events around a target reinforcing occurrence.

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“…7-12). Other future applications may include the screening of pharmacological effectors [79] and libraries for RNAi knockdown, and comparisons across inbred strains of Drosophila or across species.…”

Section: Utility For Analysing Individual Behaviourmentioning

confidence: 99%

High-resolution analysis of individualDrosophila melanogasterlarvae within groups uncovers inter- and intra-individual variability in locomotion and its neurogenetic modulation

Thane

Paisios

Stöter

et al. 2022

Preprint

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Neuronally orchestrated muscular movement and locomotion are defining faculties of multicellular animals. Due to its numerically simple brain and neuromuscular system and its genetic accessibility, the larva of the fruit fly Drosophila melanogaster is an established model to study these processes at tractable levels of complexity. However, although the faculty of locomotion clearly pertains to the individual animal, present studies of locomotion in larval Drosophila mostly use group assays and measurements aggregated across individual animals. The alternative is to measure animals one at a time, an extravagance for larger-scale analyses. In principle or in practice, this in particular rules out grasping the inter- and intra-individual variability in locomotion and its genetic and neuronal determinants. Here we present the IMBA (Individual Maggot Behaviour Analyser) for tracking and analysing the behaviour of individual larvae within groups. Using a combination of computational modelling and statistical approaches, the IMBA reliably resolves individual identity across collisions. It does not require specific hardware and can therefore be used in non-expert labs. We take advantage of the IMBA first to systematically describe the inter- and intra-individual variability in free, unconstrained locomotion in wild-type animals. We then report the discovery of a novel, complex locomotion phenotype of a mutant lacking an adhesion-type GPCR. The IMBA further allows us to determine, at the level of individual animals, the modulation of locomotion across repeated activations of dopamine neurons. Strikingly, IMBA can also be used to analyse 'silly walks', that is patterns of locomotion it was not originally designed to investigate. This is shown for the transient backward locomotion induced by brief optogenetic activation of the brain-descending 'mooncrawler' neurons, and the variability in this behaviour. Thus, the IMBA is an easy-to-use toolbox allowing an unprecedentedly rich view of the behaviour and behavioural variability of individual Drosophila larvae, with utility in multiple biomedical research contexts.

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Associative learning in larval and adult Drosophila is impaired by the dopamine-synthesis inhibitor 3-Iodo-L-tyrosine

Cited by 5 publications

References 79 publications

Optogenetically induced reward and ‘frustration’ memory in larval Drosophila

Optogenetically induced reward and ‘frustration’ memory in larval Drosophila

Optogenetically induced reward and ‘frustration’ memory in larval Drosophila melanogaster

High-resolution analysis of individualDrosophila melanogasterlarvae within groups uncovers inter- and intra-individual variability in locomotion and its neurogenetic modulation

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