Associative Genetic Diversity of RoTat 1.2 VSG in Different Trypanosoma evansi Isolates

Gaur, Ruchi Singh; Shanker, Daya; Sudan, Vikrant; Paliwal, Sanjhi; Singh, Shanker K.; Jadaun, Ashutosh

doi:10.1007/s11686-020-00273-4

Cited by 5 publications

(2 citation statements)

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“…VSG is a structural layer of glycoprotein that coats the entire cell surface of Trypanosoma sp. ( Sudan et al, 2017 ; Gaur et al, 2021 ). RoTat 1.2 is VSG’s predominant variant antigen type ( Gaur et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of new Trypanosoma evansi type non-A/B isolates from buffaloes and cattle in Indonesia

Subekti,

Suwanti,

Kurniawati

et al. 2024

Rev. Bras. Parasitol. Vet.

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Trypanosoma evansi is reportedly divided into two genotypes: types A and B. The type B is uncommon and reportedly limited to Africa: Kenya Sudan, and Ethiopia. In contrast, type A has been widely reported in Africa, South America, and Asia. However, Trypanosoma evansi type non-A/B has never been reported. Therefore, this study aims to determine the species and genotype of the Trypanozoon subgenus using a robust identification algorithm. Forty-three trypanosoma isolates from Indonesia were identified as Trypanosoma evansi using a molecular identification algorithm. Further identification showed that 39 isolates were type A and 4 isolates were possibly non-A/B types. The PML, AMN-SB1, and STENT3 isolates were likely non-A/B type Trypanosoma evansi isolated from buffalo, while the PDE isolates were isolated from cattle. Cladistic analysis revealed that Indonesian Trypanosoma evansi was divided into seven clusters based on the gRNA-kDNA minicircle gene. Clusters 6 and 7 are each divided into two sub-clusters. The areas with the highest genetic diversity are the provinces of Banten, Central Java (included Yogyakarta), and East Nusa Tenggara. The Central Java (including Yogyakarta) and East Nusa Tenggara provinces, each have four sub-clusters, while Banten has three.

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Section: Introductionmentioning

confidence: 99%

“…( Sudan et al, 2017 ; Gaur et al, 2021 ). RoTat 1.2 is VSG’s predominant variant antigen type ( Gaur et al, 2021 ). Several RoTat 1.2 primer pairs have different nucleotide sequences and have been used for different purposes, including identifying Trypanosoma evansi genotypes ( Birhanu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%