Associations with tight junction genes PARD3 and MAGI2 in Dutch patients point to a common barrier defect for coeliac disease and ulcerative colitisAn unusual case of ascites

Wapenaar, Martin C.; Monsuur, Alienke J.; Bodegraven, Ad A. van; Weersma, Rinse K.; Bevova, Marianna; Linskens, Ronald K.; Howdle, P D; Holmes, G. K. T.; Mulder, Chris J.; Dijkstra, Gerard; Heel, David A. van; Wijmenga, Cisca

doi:10.1136/gut.2007.133132

Cited by 148 publications

(144 citation statements)

References 41 publications

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“…The higher levels of IgG coating in vivo or after autologous-serum incubation in CD patients indicate a breakdown of mucosal tolerance for the intestinal bacteria, perhaps caused by a leaky bowel (34). Calprotectin levels were significantly higher in active-CD patients than in patients in remission, and both were significantly higher than those of healthy volunteers.…”

Section: Discussionmentioning

confidence: 81%

Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls

Harmsen¹,

Pouwels²,

Funke³

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTIn Crohn's disease (CD), chronic gut inflammation leads to loss of mucosal barrier integrity. Subsequent leakage of IgG to the gut could produce an increase of IgG coating of intestinal bacteria. We investigated if there is more IgG coating in patients than in volunteers and whether this is dependent on the host IgG response or on the gut bacteria. Fecal and serum samples were obtained from 23 CD patients and 11 healthy volunteers. Both thein vivoIgG-coated fecal bacteria andin vitroIgG coating after serum addition were measured by flow cytometry and related to disease activity. The bacterial composition in feces was determined using fluorescencein situhybridization. The IgG-binding capacities ofEscherichia colistrains isolated from feces of patients and volunteers were assessed. The results showed that thein vivoIgG-coated fraction of fecal bacteria of patients was slightly larger than that of volunteers but significantly larger after incubation with either autologous or heterologous serum. This was dependent on the bacteria and independent of disease activity or the serum used. The presence of moreEnterobacteriaceaeand fewer faecalibacteria in patient feces was confirmed.E. coliisolates from patients bound more IgG than isolates from volunteers (P< 0.05) after the addition of autologous serum. Together, these results indicate that CD patients have more IgG-binding gut bacteria than healthy volunteers. We showed that the level of IgG coating depends on the bacteria and not on the serum used. Furthermore, CD patients have a strong specific immune response to their ownE. colibacteria.

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Section: Discussionmentioning

confidence: 81%

Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls

Harmsen¹,

Pouwels²,

Funke³

et al. 2012

Clin. Vaccine Immunol.

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show abstract

“…80 Indeed, gut mucosal barrier dysfunction was repeatedly demonstrated and confirmed by genetic studies in patients with celiac disease and T1D. [81][82][83] Several intestinal viral triggers including adenovirus, hepatitis C virus, and rotavirus and bacterial infections capable of initiating or augmenting gut mucosal responses to gluten were suggested to play a role in the pathogenic mechanism of this disease. 84 Abnormal components found among the microbial inhabitants adhering to the diseased jejunal mucosa have been described and recently analyzed using new microbiological methods by Ou et al 85 Profound changes in the fecal and duodenal microbiota composition of patients with active disease who are on a gluten-free diet have also been demonstrated.…”

Section: Celiac Diseasementioning

confidence: 99%

The role of gut microbiota (commensal bacteria) and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer: contribution of germ-free and gnotobiotic animal models of human diseases

et al. 2011

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“…Abnormalities in func tional proteins have been observed in CD. By using genetic assoc iation analysis with a SNPs approac h, the tight junc tion permeability barrier genes, KRD 3 (2SNPs) and MAGI 2 (2SNPs), were shown to be assoc iated with CD in British and Dutc h persons [23] . In addition to the involvement of HLA classⅠ restric ted CD8+ T-c ells, the innate immune system may also be involved in CD.…”

Section: Geneticsmentioning

confidence: 99%

Recent advances in celiac disease

Freeman

Chopra²,

Clandinin³

et al. 2011

WJG

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Celiac disease now affects about one person in a hundred in Europe and North America. In this review, we consider a number of important and exciting recent developments, such as clinical associations, HLA-DQ2 and HLA-DQ8 predispositions, the concept of potential celiac disease, the use of new imaging/endoscopy techniques, and the development of refractory disease. This review will be of use to all internists, pediatricians and gastroenterologists.

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Associations with tight junction genes PARD3 and MAGI2 in Dutch patients point to a common barrier defect for coeliac disease and ulcerative colitisAn unusual case of ascites

Abstract: These results suggest that coeliac disease and ulcerative colitis may share a common aetiology through tight junction-mediated barrier defects, although the observations need further replication.

Cited by 148 publications

References 41 publications

Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls

Crohn's Disease Patients Have More IgG-Binding Fecal Bacteria than Controls

The role of gut microbiota (commensal bacteria) and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer: contribution of germ-free and gnotobiotic animal models of human diseases

Recent advances in celiac disease

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